Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,068,689 A→G 6.5% I367I (ATT→ATC aer ← fused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,068,6890AG 6.5% 357.8 / 12.9 122I367I (ATT→ATCaerfused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component
Reads supporting (aligned to +/- strand):  ref base A (59/55);  new base G (5/3);  total (64/58)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.24e-01

Only 100 of 129 total aligned reads displayed.

TGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGA  >  Exported/2068620‑2068759
                                                                     |                                                                      
tGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa                                                                       >  1:3807709/1‑71 (MQ=255)
 gCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa                                                                       >  1:3653013/1‑70 (MQ=255)
 gCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa                                                                       >  1:6106353/1‑70 (MQ=255)
 gCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa                                                                       >  1:5694116/1‑70 (MQ=255)
  ccaccaCTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAat                                                                      <  1:6837769/70‑1 (MQ=255)
  ccaccaCTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAat                                                                      <  1:4933363/70‑1 (MQ=255)
  ccaccaCTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAat                                                                      <  1:2396378/70‑1 (MQ=255)
       acTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTg                                                                  >  1:3518513/1‑69 (MQ=255)
       acTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGt                                                                >  1:1798335/1‑71 (MQ=255)
           cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTg                                                             >  1:5580555/1‑70 (MQ=255)
           cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTg                                                             >  1:2414828/1‑70 (MQ=255)
           cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGa                                                            <  1:5750823/71‑1 (MQ=255)
           cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGa                                                            <  1:1968496/71‑1 (MQ=255)
                ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGc                                                       <  1:2833395/71‑1 (MQ=255)
                ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGc                                                       <  1:4620461/71‑1 (MQ=255)
                  gcggCCCTGCTCGCCGGCGCGCGCCGCTTCCACGGCCGCGTTCAGCGCCAGGATATTGGTCTGGAAGGc                                                       <  1:6666501/66‑1 (MQ=255)
                    tGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTg                                                                  >  1:1032553/1‑56 (MQ=255)
                    tGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAata                                                   >  1:4407725/1‑71 (MQ=255)
                    gtcccTGTTCACCGGCACGGGCCGCTTCCACTGCCGCGTTGAGCGCCAGGATATTCGTCTGgaa                                                          >  1:4891847/3‑64 (MQ=255)
                        cTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGt                                               <  1:6477598/71‑1 (MQ=255)
                          gTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGtt                                              >  1:3990473/1‑70 (MQ=255)
                           ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGtt                                              <  1:5563508/69‑1 (MQ=255)
                           ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGtt                                              <  1:2592400/69‑1 (MQ=255)
                           ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTa                                             <  1:2806037/70‑1 (MQ=255)
                            gcGCCGGCGCGCGCCGCTTCCACGGCCGCGTTCAGCGCCAGGATATTGGTCTGGAAGGc                                                       <  1:1307696/58‑1 (MQ=255)
                             cGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAAt                                           <  1:839837/70‑1 (MQ=255)
                             agCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTGTGAAACGCAATATCGTTAAt                                           >  1:4377635/2‑70 (MQ=255)
                              gCCGGCGCGGGCCGCTTCCACGGCCGCGTTCAGCGCCAGGATATTGGTCTGGAAGGc                                                       <  1:4278239/57‑1 (MQ=255)
                              gCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAAt                                           <  1:1021023/69‑1 (MQ=255)
                                 ggCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCg                                      <  1:193591/71‑1 (MQ=255)
                                   cACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat                                                                    <  1:3339644/39‑1 (MQ=255)
                                   cACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat                                                                    <  1:1486447/39‑1 (MQ=255)
                                     cgcgCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTg                                                                  <  1:6407057/39‑1 (MQ=255)
                                       cgccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTaa                                >  1:244163/1‑71 (MQ=255)
                                       cgccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTaa                                >  1:4019265/1‑71 (MQ=255)
                                         ccgcTTCCACCGCCGCATTGAGCGCCAGGATATTGGTCt                                                              >  1:173502/1‑39 (MQ=37)
                                         ccgcTTCCACCGCCGCATTGAGCGCCAGGATATTGGTCt                                                              >  1:6907428/1‑39 (MQ=37)
                                         ccgcTTCCACCGCCGCATTGAGCGCCAGGATATTGGTCt                                                              >  1:23447/1‑39 (MQ=37)
                                         ccgcTTCCACCGCCGCATTGAGCGCCAGGATATTGGTCt                                                              >  1:3456985/1‑39 (MQ=37)
                                         ccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATg                              <  1:5128934/71‑1 (MQ=255)
                                           gcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGt                            <  1:1408128/71‑1 (MQ=255)
                                           gcTTCCACAGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATg                              <  1:408206/69‑1 (MQ=255)
                                            gttCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGt                                  <  1:34910/63‑1 (MQ=255)
                                            cTTCCACCGCCTCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGt                                  <  1:5459991/64‑1 (MQ=255)
                                            cTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTa                                             <  1:6135569/53‑1 (MQ=255)
                                            cTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGt                                  <  1:5801164/64‑1 (MQ=255)
                                            cTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGt                                  <  1:3442479/64‑1 (MQ=255)
                                             ttCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAg                                        >  1:2693970/1‑57 (MQ=255)
                                               ccACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGcc                         <  1:769034/70‑1 (MQ=255)
                                               ccACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGcc                         >  1:2856835/1‑70 (MQ=255)
                                                cACCGCCGCATTCAGGGCCAGAATATTGGTCTGaa                                                           >  1:739857/1‑35 (MQ=255)
                                                cACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCaa                       >  1:4325989/1‑71 (MQ=255)
                                                cACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCaa                       >  1:6472506/1‑71 (MQ=255)
                                                cACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCaa                       >  1:2529885/1‑71 (MQ=255)
                                                  ccgccgCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAg                                   >  1:1421477/1‑57 (MQ=255)
                                                  ccgccgCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGt                                  >  1:2536457/1‑58 (MQ=255)
                                                  ccgccgCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGt                                  >  1:4570626/1‑58 (MQ=255)
                                                  ccgccgCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGt                                  >  1:1482263/1‑58 (MQ=255)
                                                  ccgccgCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGt                                  >  1:6197923/1‑58 (MQ=255)
                                                  ccgccgCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGt                                  >  1:6637804/1‑58 (MQ=255)
                                                  ccgccgCATTCAGGGCAAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGt                                  >  1:3811915/1‑58 (MQ=255)
                                                  ccgccgCATTCAGGGACAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGt                                  >  1:2356183/1‑58 (MQ=255)
                                                   cgccgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATgc                    <  1:6624147/71‑1 (MQ=255)
                                                    gccgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTTATCAGCGAAGTAATGGTGCCAATgcg                   >  1:4505235/1‑71 (MQ=255)
                                                    gccgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATgcg                   >  1:3347845/1‑71 (MQ=255)
                                                    gccgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATgcg                   >  1:4746630/1‑71 (MQ=255)
                                                    gccgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATgcg                   >  1:4104935/1‑71 (MQ=255)
                                                    accgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCg                                                >  1:2565390/2‑42 (MQ=255)
                                                     ccgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGtt                                              <  1:1995126/43‑1 (MQ=255)
                                                     ccgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATgc                    <  1:1219400/69‑1 (MQ=255)
                                                     ccgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATgc                    <  1:4331124/69‑1 (MQ=255)
                                                     ccgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATgc                    <  1:5794913/69‑1 (MQ=255)
                                                           tCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTgg               >  1:540484/1‑68 (MQ=255)
                                                           tCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTa            >  1:1164713/1‑71 (MQ=255)
                                                           tCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTa            >  1:5782599/1‑71 (MQ=255)
                                                            cAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGc                                       >  1:3755518/1‑43 (MQ=255)
                                                            cAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGc                                       >  1:3881056/1‑43 (MQ=255)
                                                            cAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGaa                                    >  1:2298219/1‑46 (MQ=255)
                                                            cAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTAc           >  1:4980068/1‑71 (MQ=255)
                                                            cAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTAc           >  1:4002738/1‑71 (MQ=255)
                                                             aGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTGATGGTGCCAATGCGCTGGGTACt          >  1:1814521/1‑71 (MQ=255)
                                                             aGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACt          >  1:5211301/1‑71 (MQ=255)
                                                             aGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACt          >  1:5068808/1‑71 (MQ=255)
                                                             aGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACt          >  1:5073282/1‑71 (MQ=255)
                                                               ggCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTaa                                >  1:2651274/1‑47 (MQ=255)
                                                               ggCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAAt                               >  1:3791154/1‑48 (MQ=255)
                                                                gCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTaa                                <  1:2429987/46‑1 (MQ=255)
                                                                gCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTaa                                <  1:1490762/46‑1 (MQ=255)
                                                                gCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTaa                                <  1:4142041/46‑1 (MQ=255)
                                                                gCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTaa                                <  1:3168396/46‑1 (MQ=255)
                                                                gCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTc       >  1:6150795/1‑71 (MQ=255)
                                                                 ccAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTc       >  1:4522872/1‑70 (MQ=255)
                                                                   aGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTggcggc    <  1:82066/71‑1 (MQ=255)
                                                                   aGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCTGc    <  1:1480556/71‑1 (MQ=255)
                                                                   aGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGc    <  1:4947308/71‑1 (MQ=255)
                                                                   aGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGc    <  1:2022599/71‑1 (MQ=255)
                                                                   aGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGc    <  1:2760669/71‑1 (MQ=255)
                                                                   aGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGc    <  1:6646540/71‑1 (MQ=255)
                                                                     aaTATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGa  <  1:4007856/71‑1 (MQ=255)
                                                                     aaTATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGa  <  1:3616972/71‑1 (MQ=255)
                                                                     |                                                                      
TGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGA  >  Exported/2068620‑2068759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: