Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	62,466	A→G	5.1%	Y331Y (TAT→TAC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	62,466	0	A	G	5.1%	234.8 / 3.5	78	Y331Y (TAT→TAC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (49/25);  new base G (2/2);  total (51/27)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01

GTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTAT  >  Exported/62396‑62536                                                                       |                                                                       gTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTta                                                                        >  1:2937238/1‑71 (MQ=255) gTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTta                                                                        >  1:6728345/1‑71 (MQ=255)   tCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTtat                                                                       >  1:2076364/1‑70 (MQ=255)   tCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTtat                                                                       >  1:6565058/1‑70 (MQ=255)    cGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTtat                                                                       >  1:4125919/1‑69 (MQ=255)    cGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTtat                                                                       >  1:6075260/1‑69 (MQ=255)    cGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTta                                                                        >  1:6009856/1‑68 (MQ=255)     gAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAg                                                                    <  1:3183181/71‑1 (MQ=255)        tAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATaa                                                                     <  1:5928009/67‑1 (MQ=255)          aaaaTGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGt                                                                   >  1:1522583/1‑67 (MQ=255)          aaaaTGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGt                                                                   >  1:1309574/1‑67 (MQ=255)          aaaaTGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGt                                                                   >  1:3990966/1‑67 (MQ=255)          aaaaTGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGt                                                                   >  1:6889826/1‑67 (MQ=255)          aaaaTGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGt                                                                   >  1:4814498/1‑67 (MQ=255)          aaaaTGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGt                                                                   >  1:5227100/1‑67 (MQ=255)            aaTGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCa                                                              >  1:5404202/1‑70 (MQ=255)            aaTGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAg                                                             >  1:6787259/1‑71 (MQ=255)            aaTGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAg                                                             >  1:2338859/1‑71 (MQ=255)            aaTGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAg                                                             >  1:2615766/1‑71 (MQ=255)            aaTGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGCTTATAAg                                                                    >  1:1957779/1‑64 (MQ=255)              tGGCATGATTTCATTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAgcg                                                           <  1:5620236/71‑1 (MQ=255)              tGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAgcg                                                           <  1:2180690/71‑1 (MQ=255)              tGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAgcg                                                           <  1:694694/71‑1 (MQ=255)              tGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAgcg                                                           <  1:6758609/71‑1 (MQ=255)                 cATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATaa                                                                     >  1:6068360/1‑58 (MQ=255)                   tGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGt                                                      <  1:252925/71‑1 (MQ=255)                   tGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGt                                                      >  1:1031841/1‑71 (MQ=255)                   tGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGt                                                      <  1:4466893/71‑1 (MQ=255)                   tGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGt                                                      <  1:848712/71‑1 (MQ=255)                   tGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGt                                                      >  1:1933420/1‑71 (MQ=255)                    gATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGtt                                                     >  1:5307856/1‑71 (MQ=255)                          aGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCt                                                <  1:6394136/70‑1 (MQ=255)                              ttGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGc                                           >  1:2461105/1‑71 (MQ=255)                               tgtgGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTAt                                                  >  1:1799094/1‑63 (MQ=255)                               tgtgGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGc                                           >  1:808554/1‑70 (MQ=255)                               tgtgGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGc                                           <  1:4014366/70‑1 (MQ=255)                                 tgGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGa                                         <  1:2167280/70‑1 (MQ=255)                                  ggAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGa                                         >  1:2489917/1‑69 (MQ=255)                                   gAAGATGCGCGTGACCAGCTCGCAATCCTTGAGGTTGTACCAGGCCAGCGCCGGCTTGTCCTCGGCGaaca                                      >  1:241191/1‑69 (MQ=255)                                      gATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGaaa                                       <  1:5216757/67‑1 (MQ=255)                                      gATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAAcggc                                   >  1:6362388/1‑71 (MQ=255)                                      gATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAAcggc                                   >  1:1253820/1‑71 (MQ=255)                                        tCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTAt                                                  >  1:1082083/1‑54 (MQ=255)                                        tCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTAt                                                  >  1:5266919/1‑54 (MQ=255)                                        tCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAAcggcgg                                 >  1:5958180/1‑71 (MQ=255)                                        tCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAAcggcgg                                 >  1:1019963/1‑71 (MQ=255)                                        tCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAAcggcgg                                 >  1:6150460/1‑71 (MQ=255)                                        tCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAAcggcgg                                 >  1:3210811/1‑71 (MQ=255)                                         cTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCCGCGCAGGTTTATCTTCGGCGAAACGGCGGt                                >  1:4736683/1‑71 (MQ=255)                                         cTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGt                                >  1:607650/1‑71 (MQ=255)                                         cTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGt                                >  1:932236/1‑71 (MQ=255)                                         cTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCCGt                                >  1:6365210/1‑71 (MQ=255)                                            cGTGACCAGCTCGCAATCCTTGAGGTTGTACCAGGCCAGCGCCGGCTTGTCCTCGGCGAACATGCGGTcga                             <  1:1547846/71‑3 (MQ=255)                                                aCCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCg                                          >  1:4784393/1‑54 (MQ=255)                                                aCCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCg                                          >  1:4776020/1‑54 (MQ=255)                                                aCCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCg                                          >  1:3229676/1‑54 (MQ=255)                                                aCCAGCTCGCAATCCTTGAGGTTGTACCAGGCCAGCGCCGGCTTGTCCTCGGCGAACATGCGGTCGAtctc                         >  1:2422970/1‑71 (MQ=255)                                                 ccAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGa                                         <  1:2914662/54‑1 (MQ=255)                                                 ccAGCTCGCAATCCTTGAGGTTGTACCAGGCCAGCGCCGGCTTGTCCTCGGCGAACATGCGGTCGATCTCg                        <  1:2096503/71‑1 (MQ=255)                                                   aGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGc                                           >  1:2484461/1‑50 (MQ=255)                                                    gCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATc                                                 <  1:191269/43‑1 (MQ=255)                                                    gCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTc                               >  1:6141302/1‑61 (MQ=255)                                                    gCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTc                               >  1:6314886/1‑61 (MQ=255)                                                    gCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTc                               >  1:3597422/1‑61 (MQ=255)                                                    gCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTc                      >  1:5993921/1‑70 (MQ=255)                                                     cTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGTCGGTCAATTTCGTCCa                    <  1:2241917/71‑1 (MQ=255)                                                     cTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCTGTCAATTTCGTCCa                    <  1:2330194/71‑1 (MQ=255)                                                     cTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCa                    <  1:3561457/71‑1 (MQ=255)                                                       cGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGaaa                                       >  1:4517680/1‑50 (MQ=255)                                                       cGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCaa                             >  1:6584173/1‑60 (MQ=255)                                                       cGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCaa                             >  1:5567953/1‑60 (MQ=255)                                                        gCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCAt                   <  1:5604015/69‑1 (MQ=255)                                                         cAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCg                <  1:23250/71‑1 (MQ=255)                                                           aTCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAAcg                                     >  1:3756049/1‑48 (MQ=255)                                                           aTCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTcc                     >  1:601340/1‑64 (MQ=255)                                                           aTCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTcc                     >  1:5704933/1‑64 (MQ=255)                                                            tCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATc             >  1:5338803/1‑71 (MQ=255)                                                              tttCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTc                      <  1:6052000/60‑1 (MQ=255)                                                                tCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAAcg                                     >  1:2513290/1‑43 (MQ=255)                                                                tCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCAt                   >  1:6651069/1‑61 (MQ=255)                                                                tCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCAt                   >  1:4784659/1‑61 (MQ=255)                                                                tCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATcc            <  1:5823581/68‑1 (MQ=255)                                                                tCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCa          <  1:2528479/70‑1 (MQ=255)                                                                tCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCa          <  1:2566889/70‑1 (MQ=255)                                                                   ggTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACggg      <  1:198289/71‑1 (MQ=255)                                                                       ataAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTa   <  1:2125439/70‑1 (MQ=255)                                                                       ataAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTa   >  1:178466/1‑70 (MQ=255)                                                                       ataAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTa   >  1:3036434/1‑70 (MQ=255)                                                                       ataAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTAt  >  1:1854288/1‑71 (MQ=255)                                                                       ataAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTAt  >  1:2905641/1‑71 (MQ=255)                                                                       |                                                                       GTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTAT  >  Exported/62396‑62536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑