| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 225,827 | T→C | 8.2% | intergenic (+14/‑78) | gpt → / → frsA | guanine‑hypoxanthine phosphoribosyltransferase/hydrolase, binds to enzyme IIA(Glc) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 225,827 | 0 | T | C | 8.2% | 133.8 / 4.4 | 49 | intergenic (+14/‑78) | gpt/frsA | guanine‑hypoxanthine phosphoribosyltransferase/hydrolase, binds to enzyme IIA(Glc) |
| Reads supporting (aligned to +/- strand): ref base T (20/25); new base C (2/2); total (22/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01 | |||||||||||
GAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTG > Exported/225760‑225892 | gAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTgg < 1:1765466/70‑1 (MQ=255)gAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTgg < 1:5028230/70‑1 (MQ=255) aaCAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGc < 1:1201328/70‑1 (MQ=255) aaCAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGc < 1:4798797/70‑1 (MQ=255) aaCAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCgg > 1:348639/1‑69 (MQ=255) aaCAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCgg > 1:1643718/1‑69 (MQ=255) ccGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGcc < 1:6727811/71‑1 (MQ=255) ccGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGcc > 1:3704418/1‑71 (MQ=255) ccGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGcc < 1:5539184/71‑1 (MQ=255) gTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGccag < 1:5762608/71‑3 (MQ=255) gTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGccag < 1:1477079/71‑3 (MQ=255) tGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCggg < 1:2042658/71‑1 (MQ=255) ggCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTc < 1:4111681/70‑1 (MQ=255) gCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCtt < 1:3218264/71‑1 (MQ=255) gtATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGc > 1:1390820/1‑57 (MQ=255) gtATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTaa > 1:6914322/1‑71 (MQ=255) tATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCgttgg > 1:5593940/1‑60 (MQ=255) tATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAAc > 1:4656433/1‑71 (MQ=255) ttCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCttttt < 1:1585936/66‑1 (MQ=255) gTCCCGCCAATCTCCGGTCGCTTATCTTTTCAACGCCTg > 1:4774387/1‑39 (MQ=255) gTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTg > 1:6398691/1‑39 (MQ=255) gTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCa > 1:3459226/1‑42 (MQ=255) ccGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCtt > 1:3731137/1‑57 (MQ=255) ccGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCg > 1:1543348/1‑71 (MQ=255) aTCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTAc < 1:5859689/71‑1 (MQ=255) gTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGtt < 1:869613/71‑1 (MQ=255) tCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTAAGGCGGGTTACAATAGttt > 1:2164411/1‑71 (MQ=255) cTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTa < 1:907809/44‑1 (MQ=255) aaTCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACtt > 1:3108631/1‑46 (MQ=255) aaTCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCag > 1:6336373/1‑70 (MQ=255) aaTCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCa > 1:674856/1‑69 (MQ=255) aTCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCag > 1:6830335/1‑69 (MQ=255) tCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCttttt < 1:4025559/39‑1 (MQ=255) tCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCttttt < 1:2338840/39‑1 (MQ=255) tCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCttttt < 1:6338431/39‑1 (MQ=255) tCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCttttt < 1:559651/39‑1 (MQ=255) cTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtaag > 1:6640391/1‑71 (MQ=255) cTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtaag > 1:4128186/1‑71 (MQ=255) cTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtaag > 1:5334213/1‑71 (MQ=255) cTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtaag > 1:4906610/1‑71 (MQ=255) ttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtaagta < 1:2383408/70‑1 (MQ=255) ttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtaagt < 1:2932939/69‑1 (MQ=255) ttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtaa < 1:537430/67‑1 (MQ=255) ttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtaa < 1:1232537/67‑1 (MQ=255) ttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTAt > 1:2085448/1‑71 (MQ=255) tCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTAt < 1:6206191/70‑1 (MQ=255) aaCGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTc < 1:5023337/70‑1 (MQ=255) aaCGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTc < 1:3268487/70‑1 (MQ=255) aCGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTg < 1:3958153/71‑1 (MQ=255) | GAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTG > Exported/225760‑225892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |