Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 204,556 A→G 100% intergenic (+156/‑28) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported204,5560AG100.0% 242.6 / NA 80intergenic (+156/‑28)alaV/rrlHtRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/39);  total (41/39)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGG  >  Exported/204492‑204619
                                                                |                                                               
aaaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                            <  1:4418775/70‑1 (MQ=255)
 aaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                            >  1:1299999/1‑69 (MQ=255)
 aaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                            >  1:1851610/1‑69 (MQ=255)
 aaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCaa                                                          <  1:1096595/71‑1 (MQ=255)
 aaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCaa                                                          <  1:1558486/71‑1 (MQ=255)
   ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAg                                                         >  1:324251/1‑70 (MQ=255)
   ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAg                                                         >  1:1495363/1‑70 (MQ=255)
    tGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAg                                                         >  1:2059647/1‑69 (MQ=255)
    tGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                                                        >  1:3810064/1‑70 (MQ=255)
    tGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                                                        >  1:1641936/1‑70 (MQ=255)
    tGAAACACTGAACAATGAAAGTTGTTCGTGAGACTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                                                        >  1:3031186/1‑70 (MQ=11)
      aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc                                                     <  1:587496/71‑1 (MQ=255)
      aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc                                                     <  1:1448680/71‑1 (MQ=255)
       aaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc                                                     <  1:2275825/70‑1 (MQ=255)
           cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAg                                                         <  1:2087203/62‑1 (MQ=255)
           cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt                                                <  1:175010/71‑1 (MQ=255)
                   tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc                                                     <  1:3807271/57‑1 (MQ=255)
                   tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc                                                     <  1:418170/57‑1 (MQ=255)
                   tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc                                                     <  1:3812307/57‑1 (MQ=255)
                   tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc                                                     <  1:1656166/57‑1 (MQ=255)
                   tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                        <  1:3969235/70‑1 (MQ=19)
                    gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCggg                                            >  1:3885164/1‑66 (MQ=25)
                     aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                        >  1:533536/1‑69 (MQ=25)
                       aGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAgg                                    <  1:1454643/71‑1 (MQ=21)
                         ttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt                                  <  1:2169328/71‑1 (MQ=21)
                         ttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt                                  <  1:2986788/71‑1 (MQ=21)
                         ttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt                                  <  1:1367125/71‑1 (MQ=21)
                           gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt                                  >  1:2956841/1‑69 (MQ=25)
                           gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt                                  >  1:3473327/1‑69 (MQ=25)
                            ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                                                  <  1:4147881/52‑1 (MQ=255)
                              cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt                                                >  1:2044731/1‑52 (MQ=255)
                              cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                              <  1:1752755/70‑1 (MQ=25)
                              cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                              <  1:1882381/70‑1 (MQ=25)
                               gTGAGTCTCTCAAATTATCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt                                   <  1:620639/64‑1 (MQ=255)
                                tGAGTCTCTCAAAATTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                        <  1:4292876/58‑1 (MQ=11)
                                  aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt                          >  1:1274319/1‑70 (MQ=25)
                                  aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt                          >  1:513203/1‑70 (MQ=25)
                                     ctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg                                             >  1:1645682/1‑48 (MQ=255)
                                     ctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGa                                      >  1:1663202/1‑55 (MQ=255)
                                     ctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGc                      >  1:2109422/1‑71 (MQ=21)
                                     ctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGc                      >  1:3037941/1‑71 (MQ=21)
                                      tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                     >  1:4015399/1‑71 (MQ=21)
                                      tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                     >  1:3494164/1‑71 (MQ=21)
                                      tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                     >  1:106027/1‑71 (MQ=21)
                                        tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa                   >  1:2966372/1‑71 (MQ=21)
                                        tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa                   >  1:2858315/1‑71 (MQ=21)
                                        tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa                   >  1:1345371/1‑71 (MQ=21)
                                          aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca                 <  1:4054284/71‑1 (MQ=21)
                                          aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca                 >  1:4421273/1‑71 (MQ=21)
                                          aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa                   <  1:2474142/69‑1 (MQ=25)
                                          aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa                   <  1:3030760/69‑1 (MQ=25)
                                           aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacac                <  1:3223379/71‑1 (MQ=21)
                                           aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacac                >  1:1723784/1‑71 (MQ=21)
                                           aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacac                >  1:344793/1‑71 (MQ=21)
                                           aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacac                <  1:838108/71‑1 (MQ=21)
                                           aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacac                <  1:458031/71‑1 (MQ=21)
                                            aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACg               <  1:1870807/71‑1 (MQ=21)
                                            aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACg               <  1:1961941/71‑1 (MQ=21)
                                            aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACg               <  1:2568213/71‑1 (MQ=21)
                                            aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACg               <  1:3586785/71‑1 (MQ=21)
                                             ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACgg              >  1:4354441/1‑71 (MQ=21)
                                              tttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACgg              >  1:2866127/1‑70 (MQ=25)
                                              tttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACgg              >  1:3258000/1‑70 (MQ=25)
                                              tttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACgg              >  1:516356/1‑70 (MQ=25)
                                              tttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGt             >  1:3719004/1‑71 (MQ=21)
                                              tttCGCAACACGAAGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGt             >  1:1095600/1‑71 (MQ=255)
                                               ttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg            >  1:107182/1‑71 (MQ=21)
                                                tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg            <  1:2870973/70‑1 (MQ=25)
                                                tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg            <  1:1402753/70‑1 (MQ=25)
                                                tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg            <  1:2320403/70‑1 (MQ=25)
                                                tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg            <  1:1954049/70‑1 (MQ=25)
                                                 cGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTgg           <  1:4027958/70‑1 (MQ=25)
                                                 cGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg            <  1:4447757/69‑1 (MQ=25)
                                                  gCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGAt         >  1:3438072/1‑71 (MQ=21)
                                                     acacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacac                <  1:3547698/61‑1 (MQ=25)
                                                      cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc     >  1:1164524/1‑71 (MQ=21)
                                                      cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc     >  1:2407388/1‑71 (MQ=21)
                                                       acGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGa          >  1:2100280/1‑65 (MQ=25)
                                                        cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTg   >  1:2063396/1‑71 (MQ=21)
                                                        cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTg   >  1:1164575/1‑71 (MQ=21)
                                                           tgatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg  >  1:1894032/1‑69 (MQ=25)
                                                           tgatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg  >  1:3188561/1‑69 (MQ=25)
                                                           tgatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg  >  1:741814/1‑69 (MQ=25)
                                                            gatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca                 >  1:3103692/1‑53 (MQ=255)
                                                            gatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca                 >  1:954711/1‑53 (MQ=255)
                                                             atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGa          >  1:2927636/1‑59 (MQ=255)
                                                             atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGa          >  1:1583713/1‑59 (MQ=255)
                                                             atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGa          >  1:1491240/1‑59 (MQ=255)
                                                             atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg  <  1:377775/67‑1 (MQ=25)
                                                             atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg  <  1:2242573/67‑1 (MQ=25)
                                                             atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg  <  1:1367269/67‑1 (MQ=25)
                                                             atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg  <  1:672060/67‑1 (MQ=25)
                                                             atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg  <  1:3219381/67‑1 (MQ=25)
                                                                |                                                               
AAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGG  >  Exported/204492‑204619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: