| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 138,668 | G→A | 7.0% | S500L (TCA→TTA) | htrE ← | predicted outer membrane usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 138,668 | 0 | G | A | 7.0% | 336.9 / 10.9 | 100 | S500L (TCA→TTA) | htrE | predicted outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base G (50/43); new base A (4/3); total (54/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01 | |||||||||||
CCGTAATCTTTTTTCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTT > Exported/138602‑138733 | ccGTAATCTTTTTTCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATTCGTGAGTa < 1:3209585/71‑1 (MQ=255) aTCTTTTTTCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGa > 1:737002/1‑63 (MQ=255) cTTTTTTCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACg > 1:630053/1‑70 (MQ=255) ttttttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCa > 1:3620740/1‑71 (MQ=255) ttttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACg < 1:3159958/67‑1 (MQ=255) ttttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACg < 1:479278/67‑1 (MQ=255) ttttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATg > 1:1435694/1‑71 (MQ=255) tttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACg < 1:3165100/66‑1 (MQ=255) tttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATg > 1:4126721/1‑70 (MQ=255) tttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATg > 1:2420384/1‑70 (MQ=255) tttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATg > 1:1757488/1‑70 (MQ=255) ttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATgg < 1:3049796/70‑1 (MQ=255) ttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATgg < 1:1177864/70‑1 (MQ=255) aaaTTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCg > 1:3105421/1‑71 (MQ=255) aaaTTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCg > 1:3094467/1‑71 (MQ=255) aaaTTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCg > 1:1004124/1‑71 (MQ=255) aaaTTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCg > 1:3719719/1‑71 (MQ=255) tttCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGtt > 1:3597348/1‑71 (MQ=255) tttCAACGGTTGGTTAATACTGACCGTAACCTGATATTTCATGCGTGAGTAATAACGCATgg > 1:2151492/1‑62 (MQ=255) cAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCa > 1:4112014/1‑56 (MQ=255) cAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCa < 1:1403787/70‑1 (MQ=255) aaCGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCaa < 1:2131423/70‑1 (MQ=255) cGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCg > 1:2979259/1‑62 (MQ=255) cGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCg > 1:565893/1‑62 (MQ=255) ggttggttAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATc < 1:1607005/71‑1 (MQ=255) gttggttAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATgg < 1:2166734/54‑1 (MQ=255) tggttAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTg > 1:2954923/1‑71 (MQ=255) tggttAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTg > 1:1921383/1‑71 (MQ=255) ggttAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTg < 1:1868192/70‑1 (MQ=255) ggttAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTg < 1:110519/70‑1 (MQ=255) gttAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGtt < 1:526344/71‑1 (MQ=255) aaTACTGACCGTAACCTGATTTTTCATGCGTGAGTAAt > 1:3466460/1‑38 (MQ=255) aaTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATc > 1:279796/1‑63 (MQ=255) aaTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATc > 1:155244/1‑63 (MQ=255) aaTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATc > 1:383213/1‑63 (MQ=255) aaTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCgg > 1:2334553/1‑71 (MQ=255) aaTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCgg > 1:1623906/1‑71 (MQ=255) aaTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCgg > 1:2924489/1‑71 (MQ=255) aaTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCg < 1:713027/70‑1 (MQ=255) aTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGAt > 1:3366235/1‑61 (MQ=255) aTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGAt > 1:1416778/1‑61 (MQ=255) aTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCggg < 1:1579855/71‑1 (MQ=255) aTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCgg < 1:334817/70‑1 (MQ=255) aTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCgg < 1:2489645/70‑1 (MQ=255) aTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCgg < 1:1549132/70‑1 (MQ=255) aCTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATc < 1:4298124/60‑1 (MQ=255) cTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCaa > 1:228666/1‑55 (MQ=255) cTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCaa > 1:4057896/1‑55 (MQ=255) cTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATc > 1:548630/1‑59 (MQ=255) cTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATc > 1:984845/1‑59 (MQ=255) cTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATc > 1:2110137/1‑59 (MQ=255) cTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGAt < 1:2195673/70‑1 (MQ=255) cTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATg > 1:2544496/1‑71 (MQ=255) cTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATg > 1:478779/1‑71 (MQ=255) gACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGtt > 1:1036854/1‑71 (MQ=255) aCCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGttt < 1:3919660/71‑1 (MQ=255) aCCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGttt < 1:758373/71‑1 (MQ=255) aCCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGttt < 1:3120584/71‑1 (MQ=255) aCCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGttt < 1:1856634/71‑1 (MQ=255) aCCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGttt < 1:321674/71‑1 (MQ=255) aCCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGttt < 1:3365012/71‑1 (MQ=255) gTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTc < 1:146049/47‑1 (MQ=255) tAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACt > 1:3627984/1‑71 (MQ=255) tAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACt > 1:2555248/1‑71 (MQ=255) aCCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGAt > 1:4023860/1‑61 (MQ=255) aCCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGAt > 1:1919919/1‑61 (MQ=255) aCCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGAt > 1:1147256/1‑61 (MQ=255) aCCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTc > 1:79171/1‑66 (MQ=255) aCCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACtt > 1:747287/1‑70 (MQ=255) aCCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACtt > 1:146286/1‑70 (MQ=255) gATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTtcatc > 1:4102601/1‑70 (MQ=255) aTTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTtcatc < 1:4118998/69‑1 (MQ=255) tttttCATGCGTGAGTAATTACGCATGGATTTCGGTTCaa < 1:1033615/40‑1 (MQ=255) tttttCATGCGTGAGTAATTACGCATGGATTTCGGTTCaa < 1:1570073/40‑1 (MQ=255) tttttCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTtcatc < 1:244486/68‑1 (MQ=255) tttCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCtgg < 1:3618505/51‑1 (MQ=255) tttCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCggg < 1:4000234/51‑1 (MQ=255) tttCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAAtt < 1:1475779/70‑1 (MQ=255) atccATGCGTAATTACTCACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAAt > 1:616378/4‑69 (MQ=25) ttCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTa < 1:2923398/70‑1 (MQ=255) ttCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTa < 1:2648552/70‑1 (MQ=255) ttCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTa < 1:1417112/70‑1 (MQ=255) tCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCtt > 1:1078763/1‑42 (MQ=255) tCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCtt > 1:863713/1‑42 (MQ=255) tCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCtt > 1:2362988/1‑42 (MQ=255) tCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCtt > 1:3215845/1‑42 (MQ=255) tCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCtt > 1:1660399/1‑42 (MQ=255) ccATGCGTAATTACTCACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACtt < 1:4251833/59‑1 (MQ=25) ccATGCGTAATTACTCACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACtt < 1:2730351/59‑1 (MQ=25) ccATGCGTAATTACTCACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACtt < 1:406446/59‑1 (MQ=25) aTGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGt < 1:1069039/71‑1 (MQ=255) aTGCGTAATTACTCACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTtca > 1:3247666/1‑60 (MQ=37) aTGCGTAATTACTCACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTtca > 1:1580655/1‑60 (MQ=37) aTGCGTAATTACTCACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTtca > 1:2794561/1‑60 (MQ=37) tGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGt > 1:65728/1‑70 (MQ=255) tGCCTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGtt < 1:1331536/71‑1 (MQ=255) tGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTc < 1:105678/49‑1 (MQ=255) tGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTc < 1:1743654/49‑1 (MQ=255) tGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTc < 1:783074/49‑1 (MQ=255) tGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGt < 1:933718/66‑1 (MQ=255) | CCGTAATCTTTTTTCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTT > Exported/138602‑138733 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |