| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,825,108 | C→T | 100% | L154L (CTC→CTT) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,825,108 | 0 | C | T | 98.7% | 224.0 / ‑4.3 | 76 | L154L (CTC→CTT) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (31/44); total (31/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGATTCGCGAGTCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGGAACGCGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCA > AP009048/3825041‑3825175 | cGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGAt > 1:4939862/1‑71 (MQ=255) gATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATc < 1:4188034/71‑1 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:1307027/71‑1 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:4841797/71‑1 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:3794901/70‑1 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:2974398/70‑1 (MQ=255) cgcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTc < 1:557728/70‑1 (MQ=255) cgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCa > 1:2111461/1‑69 (MQ=255) cgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAgg < 1:4351201/71‑1 (MQ=255) gaatCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGa < 1:2676720/68‑1 (MQ=255) aatCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGaa > 1:486598/3‑71 (MQ=255) atCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAc < 1:3211332/70‑1 (MQ=255) tCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCg < 1:1901699/62‑1 (MQ=255) tCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCa > 1:49131/1‑65 (MQ=255) tCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCa > 1:4035541/1‑65 (MQ=255) tCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcg < 1:4945953/71‑1 (MQ=255) gATGGAGATTATTCAGGCTAATGGCGGGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcggg < 1:4502927/71‑3 (MQ=255) gATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcggg > 1:1258178/1‑69 (MQ=255) gATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcgg < 1:2912654/70‑2 (MQ=255) gATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcgg < 1:4375421/70‑2 (MQ=255) aTGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAgg < 1:2598413/64‑1 (MQ=255) tGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGgggg < 1:4910219/71‑1 (MQ=255) ggAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgc > 1:1833793/1‑71 (MQ=255) ggAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgc > 1:2954676/1‑71 (MQ=255) gagaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATc > 1:2046396/1‑55 (MQ=255) agaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgcgc > 1:5158911/1‑71 (MQ=255) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:4599990/1‑71 (MQ=255) tattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAc < 1:1408462/59‑1 (MQ=255) attCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTc > 1:2909494/1‑52 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCa > 1:1824494/1‑52 (MQ=255) cAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAt > 1:4052875/1‑71 (MQ=255) aGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAtt < 1:592841/71‑1 (MQ=255) ggCTAATGGCCCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAttt < 1:2476450/71‑1 (MQ=255) gctaaTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAttt < 1:2021689/67‑1 (MQ=255) ctaaTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCg > 1:1146799/3‑71 (MQ=255) ctaaTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCg < 1:1901090/69‑1 (MQ=255) aaTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTc < 1:1762238/68‑1 (MQ=255) aaTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCgg > 1:969761/1‑70 (MQ=255) tGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCg < 1:3139225/39‑1 (MQ=37) tGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcg < 1:938154/48‑1 (MQ=39) ggCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCg < 1:4367227/38‑1 (MQ=38) cgcgACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAttt < 1:2355370/62‑1 (MQ=255) gcgACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGAtt > 1:2399176/1‑69 (MQ=255) gcgACGCTTGCTGGCGTATTGATTTCGCTTGATCCTCAGGAACGGGGGCGCGGCGAGATTTCGGCGAtt > 1:80286/1‑69 (MQ=255) cgACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAg < 1:3163753/71‑1 (MQ=255) gACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAgg < 1:4849352/71‑1 (MQ=255) gACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAg > 1:353631/1‑70 (MQ=255) cGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAgg > 1:4973471/1‑69 (MQ=255) cGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGa < 1:2896662/70‑1 (MQ=255) gCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGtt > 1:1020674/1‑69 (MQ=255) gCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTg < 1:5123487/70‑1 (MQ=255) cTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGa < 1:1316577/70‑1 (MQ=255) tGGCGTATTGATTTCGCTTGATCGTCAGGAACGgggg < 1:164915/37‑1 (MQ=25) gCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCTAGATTTCGGCGATTCAGGAAGTTGAGCGt < 1:4206122/71‑1 (MQ=255) gCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCg < 1:1903097/70‑1 (MQ=255) gtattGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGa > 1:1583564/4‑71 (MQ=255) gtattGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGa > 1:3820412/4‑71 (MQ=255) tattGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGt < 1:3064294/66‑1 (MQ=255) ttGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCgaga > 1:2607080/1‑40 (MQ=38) ttGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGa > 1:328850/1‑62 (MQ=255) ttGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGAtt < 1:4008152/70‑1 (MQ=255) gATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAg > 1:3957880/1‑61 (MQ=255) gATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTAc < 1:4261465/70‑1 (MQ=255) gATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTAc < 1:663771/70‑1 (MQ=255) gATTTCGCTCGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTAc < 1:3501724/70‑1 (MQ=255) aTTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACaa < 1:3892138/71‑1 (MQ=255) aTTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACaa < 1:2118813/71‑1 (MQ=255) tttCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGt < 1:994333/62‑1 (MQ=255) tCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACt < 1:1840356/70‑1 (MQ=255) tCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTg > 1:4396959/1‑71 (MQ=255) cGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACt < 1:3234063/69‑1 (MQ=255) gCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTc > 1:444682/1‑36 (MQ=37) gCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTc > 1:3871366/1‑36 (MQ=37) gCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGc > 1:4302177/1‑70 (MQ=255) gCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGc > 1:1466578/1‑70 (MQ=255) gCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCa < 1:1327931/71‑1 (MQ=255) | CGATTCGCGAGTCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGGAACGCGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCA > AP009048/3825041‑3825175 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |