| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,825,051 | G→A | 100% | E135E (GAG→GAA) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,825,051 | 0 | G | A | 100.0% | 246.8 / NA | 83 | E135E (GAG→GAA) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (34/49); total (34/49) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAGTCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGG > AP009048/3824984‑3825118 | gtAGCGCGTTACAAGGACGCG‑AATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCg < 1:2468413/70‑1 (MQ=255) gcgcGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGAt < 1:408937/70‑1 (MQ=255) gcgcGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATg < 1:4152363/71‑1 (MQ=255) gcgcGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATg > 1:3308056/1‑71 (MQ=255) gcgcGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATg > 1:3302362/1‑71 (MQ=255) gcgcGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATg > 1:2320400/1‑71 (MQ=255) gcgTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATg > 1:644200/1‑69 (MQ=255) gcgTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATg > 1:323184/1‑69 (MQ=255) gcgTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATg > 1:1663620/1‑69 (MQ=255) gTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGAt > 1:1515076/1‑66 (MQ=255) gTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGAt > 1:2490784/1‑66 (MQ=255) tACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGAt < 1:159269/64‑1 (MQ=255) tACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGat > 1:2519876/1‑70 (MQ=255) tACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGat > 1:2458255/1‑70 (MQ=255) aCAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGatta > 1:2739721/1‑71 (MQ=255) cAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGattat < 1:3951075/71‑1 (MQ=255) cAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGatta < 1:1280690/70‑1 (MQ=255) aaGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGattatt < 1:3279978/71‑1 (MQ=255) aaGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGattatt > 1:216977/1‑71 (MQ=255) ggACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCa < 1:3228694/71‑1 (MQ=255) gACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAg < 1:3388867/71‑1 (MQ=255) aCGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAgg > 1:4432560/1‑71 (MQ=255) gcgTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGgag > 1:2851943/1‑59 (MQ=255) gcgTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGct < 1:148953/71‑2 (MQ=255) gcgTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGct < 1:3758535/71‑2 (MQ=255) aaTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGTTTCGCGAATCGATGGAGATTATTCAGGCTAATg > 1:2773843/1‑71 (MQ=255) aaTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAAt < 1:2935818/70‑1 (MQ=255) aTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATgg < 1:1547425/71‑1 (MQ=255) aTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATgg > 1:975689/1‑71 (MQ=255) cTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcg > 1:2789711/1‑70 (MQ=255) gTAGATGATGTGATCAGCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGcgcg < 1:2111416/69‑1 (MQ=255) gTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGcgcg < 1:5110054/69‑1 (MQ=255) gTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGcgcg < 1:3002781/69‑1 (MQ=255) gTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGAc > 1:4288239/1‑71 (MQ=255) tAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACg < 1:4907272/71‑1 (MQ=255) gatgatGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGc < 1:3714681/70‑1 (MQ=255) atgatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGct < 1:4073618/56‑2 (MQ=255) atgatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATg < 1:409352/60‑1 (MQ=255) atgatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCtt < 1:1294167/71‑1 (MQ=255) atgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTc < 1:3140092/48‑1 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTg > 1:4554703/1‑71 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTg < 1:3471435/71‑1 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTg > 1:4751754/1‑71 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCCCGAATCGATg > 1:1954837/1‑37 (MQ=37) gtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTgg > 1:3186607/1‑71 (MQ=255) tgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGc < 1:1002157/67‑1 (MQ=255) tgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTACTCAGGCTAATgg > 1:3625188/1‑55 (MQ=255) aTCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGct < 1:4781716/48‑2 (MQ=39) aTCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGct < 1:1661184/48‑2 (MQ=39) tCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgta < 1:5143105/71‑2 (MQ=255) tCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgta < 1:1666408/71‑2 (MQ=255) tCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgta < 1:5083493/71‑2 (MQ=255) aCCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg < 1:990384/67‑1 (MQ=255) aCCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTAtt < 1:598691/71‑1 (MQ=255) ccgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTAtt < 1:4398871/70‑1 (MQ=255) cgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGGTGGCGTATTGa > 1:890219/1‑71 (MQ=255) cgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgt > 1:5067526/1‑66 (MQ=255) cgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGa > 1:3307288/1‑71 (MQ=255) cgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGa > 1:1420943/1‑71 (MQ=255) gccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAt > 1:3659965/1‑71 (MQ=255) gccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAt < 1:567019/71‑1 (MQ=255) gcgGTACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATg < 1:2706552/44‑1 (MQ=37) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgt < 1:1176174/64‑1 (MQ=255) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgt < 1:1234933/64‑1 (MQ=255) cgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATgg > 1:3320328/1‑45 (MQ=38) gAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCg > 1:4421202/1‑71 (MQ=255) aaCGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGc < 1:2876051/71‑1 (MQ=255) aCGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCg < 1:454432/69‑1 (MQ=255) cGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt < 1:4540550/71‑2 (MQ=255) ggCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCttg < 1:4885902/71‑3 (MQ=255) ggCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt > 1:1591931/1‑69 (MQ=255) cGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGAt > 1:4939862/1‑71 (MQ=255) gATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg < 1:900332/53‑1 (MQ=39) gATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATc < 1:4188034/71‑1 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGc < 1:4451041/50‑1 (MQ=39) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:1307027/71‑1 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:4841797/71‑1 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:2974398/70‑1 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:3794901/70‑1 (MQ=255) cgcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTc < 1:557728/70‑1 (MQ=255) gcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTc > 1:2408887/1‑58 (MQ=255) cgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCa > 1:2111461/1‑69 (MQ=255) cgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAgg < 1:4351201/71‑1 (MQ=255) | GTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAGTCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGG > AP009048/3824984‑3825118 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |