| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,824,919 | G→T | 100% | L91L (CTG→CTT) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,824,919 | 0 | G | T | 98.6% | 207.5 / ‑4.1 | 70 | L91L (CTG→CTT) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (33/36); minor base A (0/1); total (33/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAAGAAGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGT > AP009048/3824853‑3824985 | cccTGCTTACAAAGTGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacc > 1:1593928/1‑71 (MQ=255)cccTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacc > 1:3472502/1‑71 (MQ=255)cccTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttga > 1:3717598/1‑69 (MQ=255) ccTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCAtgacct‑gacctg < 1:2464907/71‑1 (MQ=255) cTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctg > 1:1797215/1‑71 (MQ=255) tGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGc > 1:3930200/1‑71 (MQ=255) cTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg > 1:3303739/1‑71 (MQ=255) ttACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg > 1:2299084/1‑70 (MQ=255) aCAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGt < 1:766191/69‑1 (MQ=255) aCAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTa < 1:1022044/70‑1 (MQ=255) aaaGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACt > 1:730349/1‑70 (MQ=255) aaaGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTg > 1:5003074/1‑71 (MQ=255) aGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgac > 1:4270914/1‑58 (MQ=255) aGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgac > 1:1026169/1‑58 (MQ=255) aGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt > 1:3842773/1‑71 (MQ=255) aGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt > 1:3138110/1‑71 (MQ=255) aGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt > 1:2808094/1‑71 (MQ=255) aGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt > 1:2981782/1‑71 (MQ=255) ggATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCttt < 1:2266032/71‑1 (MQ=255) tCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAAc < 1:4258885/71‑1 (MQ=255) cccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTTCCGTACTGCTTTAAcc > 1:5101386/1‑71 (MQ=255) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctg > 1:1268162/1‑54 (MQ=25) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCg < 1:2970445/71‑1 (MQ=255) cGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAAcc > 1:616703/1‑69 (MQ=255) gATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGc > 1:1465747/1‑70 (MQ=255) gATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCa < 1:3379486/71‑1 (MQ=255) gATTGCGACCACTACCGCCGTGCCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGc > 1:4450107/1‑70 (MQ=255) tGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCa > 1:3155027/1‑68 (MQ=255) gcgaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa > 1:2370290/4‑69 (MQ=255) gaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaagagg < 1:3842048/70‑3 (MQ=255) accacTACCGCCGTGGCGCTGGCGGAGCATCCTGACCTTGACCTGCCGTACTGCTTTAACCGCAaagagg > 1:4199562/1‑68 (MQ=255) accacTACCGCCGTGGCGCTGGCGGAGCATCATgaccttga > 1:2749798/1‑41 (MQ=16) accacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc < 1:2627195/71‑1 (MQ=255) accacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc < 1:221514/71‑1 (MQ=255) ccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc > 1:1566546/1‑70 (MQ=255) ccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc > 1:1800212/1‑70 (MQ=255) ccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc > 1:2139405/1‑70 (MQ=255) cactACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:2069658/71‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCttt > 1:252124/3‑53 (MQ=37) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:2502336/69‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:4517601/69‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:501875/69‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa > 1:1627547/3‑69 (MQ=255) taCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:4923786/67‑1 (MQ=255) taCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:2607802/67‑1 (MQ=255) ccgccgTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAATAGGCAAAAGa > 1:1178309/1‑70 (MQ=255) ccgccgTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAg < 1:1888605/69‑1 (MQ=255) ccgccgTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGAc > 1:140365/1‑71 (MQ=255) ccgccgTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGAc > 1:1450498/1‑71 (MQ=255) cGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTa < 1:1004759/42‑1 (MQ=25) cGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGAcc < 1:916455/71‑1 (MQ=255) cGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGAc > 1:3291487/1‑70 (MQ=255) gcgcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTg < 1:4913456/67‑1 (MQ=255) gcgcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGa < 1:4902635/68‑1 (MQ=255) cgcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGaa < 1:4943401/69‑1 (MQ=255) gcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCttt < 1:3198096/39‑1 (MQ=38) cTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:3149869/55‑1 (MQ=255) cTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAgg > 1:5121200/1‑71 (MQ=255) tGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACgg > 1:1463398/1‑64 (MQ=255) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTa < 1:2216593/37‑1 (MQ=38) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggggg < 1:2219535/71‑1 (MQ=255) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcgg < 1:900494/71‑1 (MQ=255) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcgg < 1:3402987/71‑1 (MQ=255) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcgg < 1:1043984/71‑1 (MQ=255) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcgg > 1:4742852/1‑71 (MQ=255) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcg < 1:3770226/70‑1 (MQ=255) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggc < 1:1582570/69‑1 (MQ=255) gCGGAGCATCATGACCTAGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggc < 1:2548262/69‑1 (MQ=255) ggAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:2627005/50‑1 (MQ=255) gAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCaa > 1:2876614/1‑71 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGa > 1:4535494/1‑52 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAAt < 1:446042/71‑1 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAAt > 1:4546732/1‑71 (MQ=255) catcatGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCa > 1:4457915/1‑67 (MQ=255) catcatGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCt < 1:1691485/71‑1 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTg > 1:4838218/1‑56 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTg < 1:1041366/56‑1 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGa < 1:180695/57‑1 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtgg < 1:227643/71‑1 (MQ=255) catgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggt > 1:4395169/4‑71 (MQ=255) atgacctttacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggt > 1:2120980/3‑70 (MQ=255) atgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtcgtt > 1:4886440/3‑71 (MQ=255) tgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttg > 1:2256188/2‑71 (MQ=255) gaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCaaaa > 1:253125/1‑42 (MQ=38) gaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtg < 1:2881793/66‑1 (MQ=255) gaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGG‑GGCAATCtggttg < 1:5089260/69‑1 (MQ=255) accttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttggt < 1:889905/71‑1 (MQ=255) accttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttgg > 1:397848/1‑70 (MQ=255) accttgacatgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGAACACGGTGAAGGCGGCAATc > 1:1086625/1‑63 (MQ=255) ccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttgg > 1:3929839/1‑69 (MQ=255) | CCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAAGAAGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGT > AP009048/3824853‑3824985 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |