Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AP009048 3,685,038 C→T 100% pseudogene (21/582 nt)
pseudogene (1084/1645 nt)
ilvG ←
ilvG ←
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0090483,685,0380CT100.0% 240.9 / NA 80pseudogene (21/582 nt)
pseudogene (1084/1645 nt)
ilvG
ilvG
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/43);  total (37/43)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGCTGCGCAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCCGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGAATG  >  AP009048/3684970‑3685103
                                                                    |                                                                 
tgctgcGCAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGa                                                                 >  1:86504/1‑71 (MQ=255)
tgctgcGCAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGa                                                                 >  1:1736318/1‑71 (MQ=255)
     cgcAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgtt                                                            >  1:1818744/1‑71 (MQ=255)
       cAGCCCACATCTGGTGCTGCCCCACATCTGTTGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttgt                                                          <  1:381452/71‑1 (MQ=39)
       cAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATGCGCAGGTTTACGATCTGACAgttgt                                                          <  1:2228547/71‑1 (MQ=255)
       cAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttgt                                                          <  1:2291963/71‑1 (MQ=255)
       cAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttgt                                                          >  1:2278615/1‑71 (MQ=255)
          cccACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGtttt                                                       <  1:1400192/71‑1 (MQ=255)
          cccACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGtttt                                                       <  1:737954/71‑1 (MQ=255)
           ccACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGtttt                                                       >  1:5154583/1‑70 (MQ=255)
             acaTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaa                                                     >  1:3823606/1‑70 (MQ=255)
                tCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACa                                                               >  1:2570891/1‑57 (MQ=255)
                    gTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgt                                                             >  1:197621/1‑55 (MQ=255)
                    gTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaacaa                                               <  1:71131/69‑1 (MQ=255)
                    gTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaac                                                 <  1:2985424/67‑1 (MQ=255)
                    gTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaac                                                 <  1:1486499/67‑1 (MQ=255)
                    gTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACg                                             <  1:66012/71‑1 (MQ=255)
                       ctgcCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGgc                                           <  1:4460442/70‑1 (MQ=255)
                         gcCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGcgcg                                        >  1:2920693/1‑71 (MQ=255)
                          ccccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGcgcg                                        <  1:4085194/70‑1 (MQ=255)
                          ccccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGcgcg                                        >  1:1416327/1‑70 (MQ=255)
                          ccccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGt                                       <  1:3876734/71‑1 (MQ=255)
                            ccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAg                                     <  1:3494213/71‑1 (MQ=255)
                            ccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAg                                     <  1:3635389/71‑1 (MQ=255)
                            ccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAg                                     <  1:2501127/71‑1 (MQ=255)
                            ccACATATGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGgagctcg                                      >  1:664409/1‑67 (MQ=255)
                              agaTCTGTTGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttgt                                                          <  1:1996752/46‑1 (MQ=37)
                              acaTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGtttt                                                       <  1:1290519/51‑1 (MQ=255)
                              acaTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGAt                                   <  1:2021315/71‑1 (MQ=255)
                               caTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGttt                                                        >  1:1475310/1‑49 (MQ=255)
                               caTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATa                                  <  1:4524933/71‑1 (MQ=255)
                                aTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGa                                                                 >  1:616168/1‑39 (MQ=255)
                                aTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGa                                                                 >  1:3447967/1‑39 (MQ=255)
                                aTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaa                                                     >  1:4118713/1‑51 (MQ=255)
                                 tCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaa                                                  >  1:213339/1‑53 (MQ=255)
                                 tCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaa                                                  >  1:3666909/1‑53 (MQ=255)
                                 tCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaac                                                    >  1:1347199/1‑51 (MQ=255)
                                  cTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaacaa                                               >  1:4584324/1‑55 (MQ=255)
                                  cTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCg                               >  1:546038/1‑71 (MQ=255)
                                   tgtgGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGTCGCGTa                                      <  1:1476039/63‑1 (MQ=255)
                                     tgGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttgt                                                          <  1:2608778/41‑1 (MQ=255)
                                     tgGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGc                                <  1:403566/67‑1 (MQ=255)
                                       gTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCAc                           <  1:4127846/70‑1 (MQ=255)
                                        tCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGtttt                                                       <  1:2412248/41‑1 (MQ=255)
                                        tCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCAc                           <  1:2960981/69‑1 (MQ=255)
                                         cACGACGCAATCCGCAGGTTTACGATCTGACAgttgtt                                                         <  1:4400301/38‑1 (MQ=255)
                                         cACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGAAAGCGTCACCgg                        >  1:5059454/1‑71 (MQ=255)
                                           cgacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGa                      >  1:2400937/1‑71 (MQ=255)
                                           cgacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGa                      >  1:2731640/1‑71 (MQ=255)
                                           cgacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGa                      <  1:3454379/71‑1 (MQ=255)
                                            gacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaac                                                 <  1:198479/43‑1 (MQ=255)
                                            gacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGAt                     >  1:627684/1‑71 (MQ=255)
                                            gacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGAt                     >  1:3617037/1‑71 (MQ=255)
                                              cgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGa                                    >  1:3837701/1‑54 (MQ=255)
                                               gCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGt                  >  1:4265177/1‑71 (MQ=255)
                                                 aaTCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCg                >  1:445529/1‑71 (MQ=255)
                                                  aTCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGt               <  1:2927421/71‑1 (MQ=255)
                                                   tCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGa                                    >  1:3812608/1‑49 (MQ=255)
                                                    ccGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGGGCGTAGATAGCGTCACCGGGATGGTCGTaa             >  1:4002557/1‑71 (MQ=255)
                                                    ccGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATgg                   <  1:5109519/65‑1 (MQ=255)
                                                     cGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAAc            >  1:532558/1‑71 (MQ=255)
                                                      gCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACg           <  1:4236911/71‑1 (MQ=255)
                                                       cAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGc          <  1:2682937/71‑1 (MQ=255)
                                                       cAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGc          >  1:4897482/1‑71 (MQ=255)
                                                        aGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGcc         <  1:1640839/71‑1 (MQ=255)
                                                         ggTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCa        <  1:1591182/71‑1 (MQ=255)
                                                         ggTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCa        <  1:2848363/71‑1 (MQ=255)
                                                          gTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGt                                       <  1:706093/39‑1 (MQ=255)
                                                          gTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTa                                      <  1:4229315/40‑1 (MQ=255)
                                                          gTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGAt                                   >  1:2798688/1‑43 (MQ=255)
                                                          gTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCa        <  1:3979934/70‑1 (MQ=255)
                                                          gTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAg       <  1:1047837/71‑1 (MQ=255)
                                                           tttACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAgg      <  1:1956164/71‑1 (MQ=255)
                                                            ttACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGc                                >  1:4293448/1‑44 (MQ=255)
                                                            ttACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAgg      >  1:2076939/1‑70 (MQ=255)
                                                             tACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAggc     <  1:2095399/70‑2 (MQ=255)
                                                               cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCAt   <  1:3564706/70‑1 (MQ=255)
                                                               cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATg  >  1:5188574/1‑71 (MQ=255)
                                                               cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATg  >  1:2930544/1‑71 (MQ=255)
                                                                 aTCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTaa             <  1:140433/58‑1 (MQ=255)
                                                                    |                                                                 
TGCTGCGCAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCCGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGAATG  >  AP009048/3684970‑3685103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: