Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | AP009048 | 3,685,038 | C→T | 100% | pseudogene (21/582 nt) pseudogene (1084/1645 nt) |
ilvG ← ilvG ← |
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AP009048 | 3,685,038 | 0 | C | T | 100.0% | 240.9 / NA | 80 | pseudogene (21/582 nt) pseudogene (1084/1645 nt) | ilvG ilvG | ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (37/43); total (37/43) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TGCTGCGCAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCCGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGAATG > AP009048/3684970‑3685103 | tgctgcGCAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGa > 1:86504/1‑71 (MQ=255) tgctgcGCAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGa > 1:1736318/1‑71 (MQ=255) cgcAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgtt > 1:1818744/1‑71 (MQ=255) cAGCCCACATCTGGTGCTGCCCCACATCTGTTGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttgt < 1:381452/71‑1 (MQ=39) cAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATGCGCAGGTTTACGATCTGACAgttgt < 1:2228547/71‑1 (MQ=255) cAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttgt < 1:2291963/71‑1 (MQ=255) cAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttgt > 1:2278615/1‑71 (MQ=255) cccACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGtttt < 1:1400192/71‑1 (MQ=255) cccACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGtttt < 1:737954/71‑1 (MQ=255) ccACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGtttt > 1:5154583/1‑70 (MQ=255) acaTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaa > 1:3823606/1‑70 (MQ=255) tCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACa > 1:2570891/1‑57 (MQ=255) gTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgt > 1:197621/1‑55 (MQ=255) gTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaacaa < 1:71131/69‑1 (MQ=255) gTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaac < 1:2985424/67‑1 (MQ=255) gTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaac < 1:1486499/67‑1 (MQ=255) gTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACg < 1:66012/71‑1 (MQ=255) ctgcCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGgc < 1:4460442/70‑1 (MQ=255) gcCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGcgcg > 1:2920693/1‑71 (MQ=255) ccccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGcgcg < 1:4085194/70‑1 (MQ=255) ccccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGcgcg > 1:1416327/1‑70 (MQ=255) ccccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGt < 1:3876734/71‑1 (MQ=255) ccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAg < 1:3494213/71‑1 (MQ=255) ccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAg < 1:3635389/71‑1 (MQ=255) ccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAg < 1:2501127/71‑1 (MQ=255) ccACATATGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGgagctcg > 1:664409/1‑67 (MQ=255) agaTCTGTTGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttgt < 1:1996752/46‑1 (MQ=37) acaTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGtttt < 1:1290519/51‑1 (MQ=255) acaTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGAt < 1:2021315/71‑1 (MQ=255) caTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGttt > 1:1475310/1‑49 (MQ=255) caTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATa < 1:4524933/71‑1 (MQ=255) aTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGa > 1:616168/1‑39 (MQ=255) aTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGa > 1:3447967/1‑39 (MQ=255) aTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaa > 1:4118713/1‑51 (MQ=255) tCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaa > 1:213339/1‑53 (MQ=255) tCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaa > 1:3666909/1‑53 (MQ=255) tCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaac > 1:1347199/1‑51 (MQ=255) cTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaacaa > 1:4584324/1‑55 (MQ=255) cTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCg > 1:546038/1‑71 (MQ=255) tgtgGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGTCGCGTa < 1:1476039/63‑1 (MQ=255) tgGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttgt < 1:2608778/41‑1 (MQ=255) tgGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGc < 1:403566/67‑1 (MQ=255) gTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCAc < 1:4127846/70‑1 (MQ=255) tCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGtttt < 1:2412248/41‑1 (MQ=255) tCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCAc < 1:2960981/69‑1 (MQ=255) cACGACGCAATCCGCAGGTTTACGATCTGACAgttgtt < 1:4400301/38‑1 (MQ=255) cACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGAAAGCGTCACCgg > 1:5059454/1‑71 (MQ=255) cgacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGa > 1:2400937/1‑71 (MQ=255) cgacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGa > 1:2731640/1‑71 (MQ=255) cgacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGa < 1:3454379/71‑1 (MQ=255) gacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaac < 1:198479/43‑1 (MQ=255) gacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGAt > 1:627684/1‑71 (MQ=255) gacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGAt > 1:3617037/1‑71 (MQ=255) cgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGa > 1:3837701/1‑54 (MQ=255) gCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGt > 1:4265177/1‑71 (MQ=255) aaTCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCg > 1:445529/1‑71 (MQ=255) aTCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGt < 1:2927421/71‑1 (MQ=255) tCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGa > 1:3812608/1‑49 (MQ=255) ccGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGGGCGTAGATAGCGTCACCGGGATGGTCGTaa > 1:4002557/1‑71 (MQ=255) ccGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATgg < 1:5109519/65‑1 (MQ=255) cGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAAc > 1:532558/1‑71 (MQ=255) gCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACg < 1:4236911/71‑1 (MQ=255) cAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGc < 1:2682937/71‑1 (MQ=255) cAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGc > 1:4897482/1‑71 (MQ=255) aGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGcc < 1:1640839/71‑1 (MQ=255) ggTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCa < 1:1591182/71‑1 (MQ=255) ggTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCa < 1:2848363/71‑1 (MQ=255) gTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGt < 1:706093/39‑1 (MQ=255) gTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTa < 1:4229315/40‑1 (MQ=255) gTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGAt > 1:2798688/1‑43 (MQ=255) gTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCa < 1:3979934/70‑1 (MQ=255) gTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAg < 1:1047837/71‑1 (MQ=255) tttACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAgg < 1:1956164/71‑1 (MQ=255) ttACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGc > 1:4293448/1‑44 (MQ=255) ttACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAgg > 1:2076939/1‑70 (MQ=255) tACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAggc < 1:2095399/70‑2 (MQ=255) cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCAt < 1:3564706/70‑1 (MQ=255) cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATg > 1:5188574/1‑71 (MQ=255) cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATg > 1:2930544/1‑71 (MQ=255) aTCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTaa < 1:140433/58‑1 (MQ=255) | TGCTGCGCAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCCGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGAATG > AP009048/3684970‑3685103 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |