| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,684,513 | G→A | 100% | pseudogene (546/582 nt) pseudogene (1609/1645 nt) |
ilvG ← ilvG ← |
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,684,513 | 0 | G | A | 100.0% | 155.8 / NA | 53 | pseudogene (546/582 nt) pseudogene (1609/1645 nt) | ilvG ilvG | ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/27); total (26/27) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAGCGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCGCCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTATGGCC > AP009048/3684446‑3684577 | aaGCGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCa < 1:4399215/71‑1 (MQ=255) aGCGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCAcc < 1:855666/69‑1 (MQ=255) aGCGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCa < 1:2461505/70‑1 (MQ=255) aGCGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAg < 1:2946722/71‑1 (MQ=255) cGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAgg < 1:2714185/70‑1 (MQ=255) gAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAggcg < 1:1531470/71‑1 (MQ=255) ccGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCa > 1:230406/1‑71 (MQ=255) gATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCAc > 1:4162983/1‑70 (MQ=255) aTACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCAc > 1:437010/1‑69 (MQ=255) tACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAg < 1:324607/71‑1 (MQ=255) tACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAg < 1:805335/71‑1 (MQ=255) aCATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGc > 1:4979484/1‑71 (MQ=255) aCATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGc > 1:145136/1‑71 (MQ=255) cATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGc < 1:5068680/70‑1 (MQ=255) ttGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAggcg < 1:3222125/60‑1 (MQ=255) ttGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGc > 1:1874678/1‑71 (MQ=255) tGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCg > 1:3144579/1‑68 (MQ=255) aCCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGGATTACTGGCACCAGGCGGCACCAGCGGCCAg < 1:1339053/71‑1 (MQ=255) aCCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGc < 1:4118228/68‑1 (MQ=255) cTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGAc < 1:981010/71‑1 (MQ=255) tGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAggc < 1:3933022/53‑1 (MQ=255) gCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCa < 1:3149075/70‑1 (MQ=255) catcatGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCa < 1:4124608/52‑1 (MQ=255) atcatGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGt > 1:122927/1‑71 (MQ=255) atcatGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGt > 1:879436/1‑71 (MQ=255) atcatGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGt < 1:1439798/71‑1 (MQ=255) atGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGtt > 1:2957765/1‑69 (MQ=255) gATAATTTCTCCAACATTTCTGAATTACTGGCACCa > 1:3723723/1‑36 (MQ=255) gATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGc > 1:1875282/1‑51 (MQ=255) gATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGAc < 1:142378/56‑1 (MQ=255) aTAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtcgtc < 1:1524536/70‑1 (MQ=255) tAATTTCTCCAACATTTCTGAATTACTGGCACCAgg > 1:2826033/1‑36 (MQ=255) tAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCgg > 1:3335752/1‑48 (MQ=255) aTTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtcgt > 1:4949713/1‑66 (MQ=255) aTTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAtt < 1:2158255/71‑1 (MQ=255) tttCTCCAACATTTCTGAATTACTGGCACCAggcgg < 1:4462624/36‑1 (MQ=255) tttCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAtt > 1:3862117/1‑70 (MQ=255) tctcCAACATTTCTGAATTACTGGCACCAGGCGGCAc > 1:2581282/1‑37 (MQ=255) tctcCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCaa > 1:4083116/1‑57 (MQ=255) tctcCAACATTTCTGAATTACTAGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAttag < 1:2260272/70‑3 (MQ=255) cAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACAt > 1:2087192/1‑71 (MQ=255) aCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGt > 1:1357981/1‑45 (MQ=255) aCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGa < 1:2444305/71‑1 (MQ=255) aCATTTCTGAATTACTGGCACCAGGCGCCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATg > 1:860250/1‑70 (MQ=255) tGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAgg > 1:1724642/1‑70 (MQ=255) aaTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGa < 1:3699862/51‑1 (MQ=255) aaTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGt < 1:4506354/69‑1 (MQ=255) aaTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTAt > 1:4702004/1‑71 (MQ=255) aTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACAt < 1:212392/59‑1 (MQ=255) aCTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCTTCGATTGAGACATGAAGCAGGTATGGc < 1:4475884/70‑1 (MQ=255) aCTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTga > 1:1733413/1‑51 (MQ=255) aCTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTATGGcc > 1:5170260/1‑71 (MQ=255) ggCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAtt > 1:154957/1‑46 (MQ=255) | AAGCGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCGCCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTATGGCC > AP009048/3684446‑3684577 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |