New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP009048 = 400181194 (1.670)3 (0.060) 3/92 NT 3.3% coding (963/1500 nt) pitA phosphate transporter, low‑affinity
?AP009048 = 4001825 91 (1.790)coding (949/1500 nt) pitA phosphate transporter, low‑affinity
Rejected: Frequency below/above cutoff threshold.

ACAGCATAATGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AP009048/4001738‑4001811
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcaacCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGA  <  AP009048/4001825‑4001756
                                                                                                                                                
ACAGCATAATGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTC                                                                           <  1:483892/71‑1
 CAGCATAATGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTC                                                                           >  1:3938242/1‑70
  AGCATAATGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAA                                                                         >  1:2574021/1‑71
          GCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTC                                                                           <  1:2926852/61‑1
                      GCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCAGTGGTCAACCGCCTGAAAGGTATGTT                                                       <  1:1227105/69‑1
                                    GCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACCGCCTGAAAGGTA                                                           <  1:54523/51‑1
                                        CAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACCGCCTGAAAGGTATGTTGA                                                     >  1:1907071/1‑53
                                                                     TCAACCGCCTGAAAGGTATGTTGACCACCGATGTGG                                         >  1:1506397/1‑36
                                                                      CAACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGC      >  1:1905321/1‑70
                                                                      CAACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGC                    <  1:2421441/56‑1
                                                                      CAACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCT                         >  1:4458436/1‑51
                                                                       AACCGCCTGAAATGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCA    <  1:906022/71‑1
                                                                       AACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCA    <  1:1595150/71‑1
                                                                       AACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCA    <  1:548140/71‑1
                                                                       AACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCA    <  1:1071929/71‑1
                                                                       AACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCA    <  1:4164260/71‑1
                                                                       AACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGC      <  1:99824/69‑1
                                                                       AACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGC      >  1:2265690/1‑69
                                                                       AACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCA             <  1:1100599/62‑1
                                                                       AACCGCCTGAAAGGTATGTTGACCACCGATGTGGAA                                       >  1:4016658/1‑36
                                                                        ACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAG   <  1:310589/71‑1
                                                                        ACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAG   <  1:10139/71‑1
                                                                        ACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGC      <  1:423143/68‑1
                                                                        ACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGC      >  1:2883064/1‑68
                                                                        ACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTA                                  <  1:4401984/40‑1
                                                                         CCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGA  <  1:1041011/71‑1
                                                                         CCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGC      >  1:3867367/1‑67
                                                                         CCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGC                          >  1:938700/1‑47
                                                                         CCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTA                                  >  1:2911677/1‑39
                                                                                                                                                
ACAGCATAATGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AP009048/4001738‑4001811
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcaacCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGA  <  AP009048/4001825‑4001756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.