New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP009048 952526 =66 (1.170)4 (0.090) 4/82 NT 7.3% coding (1451/2283 nt) pflB pyruvate formate lyase I
?AP009048 952557 = 48 (1.060)coding (1420/2283 nt) pflB pyruvate formate lyase I

AGGTTGGTCCGAAGTCTGAACCGATCAAAGGCGATGTCCTGAACTATGATGAAGTGATGGAGCGCATGGATCACTTCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AP009048/952604‑952526
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atcacttcatCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAA  >  AP009048/952557‑952625
                                                                                                                                                    
AGGTTGGTCCGAAGTCTGAACCGATCAAAGGCGATGTCCTGAACTATGATGAAGTGATGGAGCGCATGGAT                                                                               >  1:1066778/1‑71
AGGTTGGTCCGAAGTCTGAACCGATCAAAGGCGATGTCCTGAACTATGATGAAGTGATGGAGCGCATGGAT                                                                               <  1:342715/71‑1
  GTTGGTCCGAAGTCTGAACCGATCAAAGGCGATGTCCTGAACTATGATGAAGTGATGGAGCGCATGGATCA                                                                             >  1:5127151/1‑71
    TGGTCCGAAGTCTGAACCGATCAAAGGCGATGTCCTGAACTATGATGAAGTGATGGAGCGCATGGATCACT                                                                           >  1:3707190/1‑71
      GTCCGAAGTCTGAACCGATCAAAGGCGATGTCCTGAACTATGATGAAGTGATGGAGCGCATGGATCACTTC                                                                         <  1:2945160/71‑1
      GTCCGAAGTCTGAACCGATCAAAGGCGATGTCCTGAACTATGATGAAGTGATGGAGCGCATGGATCACTTC                                                                         <  1:4560939/71‑1
       TCCGAAGTCTGAACCGATCAAAGGCGATGTCCTGAACTATGATGAAGTGATGGAGCGCATGGATCACTTCA                                                                        >  1:1458351/1‑71
       TCCGAAGTCTGAACCGATCAAAGGCGATGTCCTGAACTATGATGAAGTGATGGAGCGCATGGATCACT                                                                           <  1:684125/68‑1
         CGAAGTCTGAACCGATCAAAGGCGATGTCCTGAACTATGATGAAGTGATGGAGCGCATGGATCACTTCAT                                                                       <  1:1514335/70‑1
         CGAAGTCTGAACCGATCAAAGGCGATGTCCTGAACTATGATGAAGTGATGGAGCGCATGGATCA                                                                             >  1:3961256/1‑64
           AAGTCTGAACCGATCAAAGGCGATGTCCTGAACTATGATGAAGTGATGGAGCGCATGGATCACTTC                                                                         >  1:3141673/1‑66
              TCTGAACCGATCAAAGGCGATGTCCTGAACTATGATGAAGTGATGGAGCGCATGGATCACTT                                                                          <  1:3225377/62‑1
                   ACCGATCAAAGGCGATGTCCTGAACTATGATGAAGTGATGGAGCGCATGGATCACT                                                                           >  1:139655/1‑56
                    CCGATCAAAGGCGATGTCCTGAACTATGATGAAGTGATGGAGCGCATGGATCACTTCAT                                                                       >  1:4476823/1‑59
                                  TGTCCTGAACTATGATGAAGTGATGGAGCGCATGGATCA                                                                             >  1:1384552/1‑39
                                                  GAAGTGATGGAGCGCATGGATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGAC                             >  1:2059175/1‑71
                                                  GAAGTGATGGAGCGCATGGATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCG                                <  1:2260106/68‑1
                                                           GAGCGCATGGATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCA                    <  1:2336535/71‑1
                                                            AGCGCATGGATCACTTCATCATAGTTCAGGACATCGCCTTT                                                 >  1:3383845/1‑41
                                                                     ATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGT            >  1:548153/1‑69
                                                                     ATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTG                      <  1:2686649/59‑1
                                                                      TCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTT         <  1:3187173/71‑1
                                                                      TCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGG                               <  1:959301/49‑1
                                                                      TCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTC                                 >  1:3396069/1‑47
                                                                       CACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCT                       <  1:4970485/56‑1
                                                                        ACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTC       >  1:1488615/1‑71
                                                                         CTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCG                                <  1:1423563/45‑1
                                                                         CTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTC                                 <  1:4514370/44‑1
                                                                           TCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTC       >  1:705571/1‑68
                                                                             ATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAA  >  1:599586/1‑71
                                                                             ATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAA  >  1:1290991/1‑71
                                                                             ATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCA   <  1:3147219/70‑1
                                                                             ATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCA   <  1:2878793/70‑1
                                                                             ATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCA   <  1:178239/70‑1
                                                                                                                                                    
AGGTTGGTCCGAAGTCTGAACCGATCAAAGGCGATGTCCTGAACTATGATGAAGTGATGGAGCGCATGGATCACTTCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AP009048/952604‑952526
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atcacttcatCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAA  >  AP009048/952557‑952625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.