Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,763,636 A→G 23.3% noncoding (208/1542 nt) rrsG ← 16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,763,6360AG23.3% 55.8 / 15.4 30noncoding (208/1542 nt)rrsG16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (9/14);  new base G (5/2);  total (14/16)
Fisher's exact test for biased strand distribution p-value = 2.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

GCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCAT  >  Exported/1763567‑1763705
                                                                     |                                                                     
gCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAg                                                                      >  1:315252/1‑71 (MQ=255)
   ttGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTc                                                                   >  1:178832/1‑71 (MQ=255)
          gCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCctct                                                            <  1:482710/71‑1 (MQ=255)
          gCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCctc                                                             <  1:1342247/70‑1 (MQ=40)
                aCCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTc                                                      >  1:200395/1‑71 (MQ=255)
                aCCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAg                                                                      >  1:8200/1‑55 (MQ=255)
                 ccccACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTc                                                      <  1:1506417/70‑1 (MQ=40)
                    caccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCTTg                                                   >  1:1754783/1‑70 (MQ=255)
                    caccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCTTGc                                                  >  1:1563970/1‑71 (MQ=255)
                       caacaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGt                                                                    <  1:763135/50‑1 (MQ=255)
                        aacaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACa                                              <  1:1439655/71‑1 (MQ=38)
                         acaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCTTGCGAcgt                                             <  1:412254/71‑3 (MQ=9)
                         acaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCTTGCGAcgt                                             >  1:1881482/1‑69 (MQ=9)
                          caaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACAt                                             >  1:1681103/1‑70 (MQ=37)
                             gCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATg                                         <  1:1988121/71‑1 (MQ=34)
                                 aTCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCgg                                      <  1:1701684/70‑1 (MQ=35)
                                      aTCTGGGCACATCCGATGGCAAGAGGGCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAg                                <  1:2040953/71‑1 (MQ=255)
                                       tCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGc                               >  1:768523/1‑71 (MQ=34)
                                         tGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTa                             >  1:675085/1‑71 (MQ=34)
                                           ggCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCt                              <  1:313693/68‑1 (MQ=35)
                                           ggCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTAcc                           <  1:290930/71‑1 (MQ=34)
                                                aTCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACAt                                             <  1:194234/48‑1 (MQ=35)
                                                aTCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCt                              <  1:504842/63‑1 (MQ=35)
                                                 tCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACa                                              <  1:1787604/46‑1 (MQ=32)
                                                  ccGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTa                                 >  1:671207/1‑58 (MQ=35)
                                                     aTGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCag                   >  1:414178/1‑69 (MQ=35)
                                                      tGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCTTTTCCa                    <  1:563245/67‑1 (MQ=25)
                                                            gagGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCag                   <  1:1629624/62‑1 (MQ=35)
                                                             agGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATcc          >  1:545373/1‑70 (MQ=35)
                                                                  cGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTa                             >  1:1631804/1‑46 (MQ=38)
                                                                     aGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCACCCTCCAt  >  1:1680850/1‑70 (MQ=25)
                                                                     |                                                                     
GCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCAT  >  Exported/1763567‑1763705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: