Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 204,556 A→G 100% intergenic (+156/‑28) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported204,5560AG100.0% 116.4 / NA 40intergenic (+156/‑28)alaV/rrlHtRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/21);  total (19/21)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGG  >  Exported/204495‑204619
                                                             |                                                               
ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAg                                                         >  1:1816280/1‑70 (MQ=255)
  gAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa                                                       <  1:1630261/70‑1 (MQ=255)
   aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaaa                                                      <  1:1422819/70‑1 (MQ=255)
    aaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACa                                                    >  1:36153/1‑71 (MQ=255)
          gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg                                              >  1:756321/1‑71 (MQ=255)
             caatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaaa                                                      >  1:714988/1‑60 (MQ=11)
              aatgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGtt                                          >  1:149846/4‑71 (MQ=255)
              aatgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGtt                                          <  1:1947742/68‑1 (MQ=255)
                tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                        <  1:137088/70‑1 (MQ=19)
                 gAAAGTTGTTCGTGAGTCTCTCTAATTTTCGCAACACGATGATGGATCGCAAGAAACAt                                                   >  1:428728/1‑59 (MQ=11)
                 gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                        >  1:2113483/1‑70 (MQ=25)
                   aaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg                                     >  1:1704800/1‑71 (MQ=21)
                   aaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg                                     >  1:2081765/1‑71 (MQ=21)
                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                            <  1:266709/46‑1 (MQ=12)
                     gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                            <  1:1641869/46‑1 (MQ=12)
                        gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt                                   >  1:338639/1‑68 (MQ=25)
                           cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                              <  1:1103411/70‑1 (MQ=25)
                            gTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCg                             <  1:1022853/70‑1 (MQ=25)
                               aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt                          >  1:1261383/1‑70 (MQ=25)
                                    ctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                        <  1:1303488/51‑1 (MQ=255)
                                     tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                     <  1:858782/69‑1 (MQ=25)
                                       aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca                 >  1:555281/1‑71 (MQ=21)
                                       aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca                 >  1:1892220/1‑71 (MQ=21)
                                       aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca                 >  1:950795/1‑71 (MQ=21)
                                        aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacac                <  1:1336573/71‑1 (MQ=21)
                                            ttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg            >  1:292749/1‑71 (MQ=21)
                                               gCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGa          <  1:1871550/70‑1 (MQ=25)
                                               gCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGAt         <  1:658510/71‑1 (MQ=21)
                                                cAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                     <  1:1080845/58‑1 (MQ=255)
                                                 aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                     <  1:773691/57‑1 (MQ=255)
                                                 aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg            <  1:501565/66‑1 (MQ=25)
                                                  acacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCg                             >  1:538927/1‑48 (MQ=255)
                                                  acacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCg                             >  1:976074/1‑48 (MQ=255)
                                                  acacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGcc      >  1:639370/1‑71 (MQ=21)
                                                  acacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGcc      <  1:425696/71‑1 (MQ=21)
                                                   cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAg                       <  1:934780/53‑1 (MQ=255)
                                                   cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc     <  1:377838/71‑1 (MQ=21)
                                                     cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTg   >  1:9101/1‑71 (MQ=21)
                                                      gatgatGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg  <  1:928289/71‑1 (MQ=21)
                                                      gatgatGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg  <  1:930933/71‑1 (MQ=21)
                                                             |                                                               
TTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGG  >  Exported/204495‑204619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: