Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 204,511 C→T 100% intergenic (+111/‑73) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported204,5110CT100.0% 10.4 / NA 5intergenic (+111/‑73)alaV/rrlHtRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/2);  total (3/2)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCG  >  Exported/204495‑204575
                |                                                                
ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAg             >  1:1816280/1‑70 (MQ=255)
  gAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa           <  1:1630261/70‑1 (MQ=255)
   aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaaa          <  1:1422819/70‑1 (MQ=255)
    aaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACa        >  1:36153/1‑71 (MQ=255)
          gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg  >  1:756321/1‑71 (MQ=255)
             caatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaaa          >  1:714988/1‑60 (MQ=11)
                |                                                                
TTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCG  >  Exported/204495‑204575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: