Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,061,535 A→C 5.3% V14V (GTA→GTC rpsU → 30S ribosomal subunit protein S21

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,061,5350AC 5.3% 265.8 / 9.3 95V14V (GTA→GTCrpsU30S ribosomal subunit protein S21
Reads supporting (aligned to +/- strand):  ref base A (48/42);  new base C (3/2);  total (51/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

CGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTC  >  Exported/2061469‑2061604
                                                                  |                                                                     
cGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGctc                                                                   <  1:4317462/71‑1 (MQ=255)
cGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGctc                                                                   <  1:435675/71‑1 (MQ=255)
cGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGctc                                                                   <  1:4012498/71‑1 (MQ=255)
 gAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGctct                                                                  <  1:2179505/71‑1 (MQ=255)
 gAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGctct                                                                  >  1:193155/1‑71 (MQ=255)
 gAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGctct                                                                  <  1:1069081/71‑1 (MQ=255)
     tAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGt                                                              >  1:3391364/1‑71 (MQ=255)
      aaTCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTc                                                             >  1:274622/1‑71 (MQ=255)
         cAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCt                                                          >  1:3953842/1‑71 (MQ=255)
          aaaGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGctct                                                                  >  1:2954023/1‑62 (MQ=255)
          aaaGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGctct                                                                  >  1:1353859/1‑62 (MQ=255)
          aaaGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCtt                                                         >  1:923647/1‑71 (MQ=255)
          aaaGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCtt                                                         <  1:4667490/71‑1 (MQ=255)
          aaaGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCtt                                                         >  1:1573189/1‑71 (MQ=255)
          aaaGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCtt                                                         >  1:1342936/1‑71 (MQ=255)
           aaGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAGACGAGCCGTTCGACGTAGCTCTGCGTCGCTTc                                                        <  1:1521630/71‑1 (MQ=255)
             ggTGAGAGGCACATGCCGGTTATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCaa                                                      <  1:619816/71‑1 (MQ=255)
             ggTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCaa                                                      <  1:167638/71‑1 (MQ=255)
                gagaGGCTCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCa                                                       <  1:2361297/67‑1 (MQ=255)
                gagaGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCa                                                       <  1:1985972/67‑1 (MQ=255)
                gagaGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCa                                                       <  1:572986/67‑1 (MQ=255)
                gagaGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCg                                                   >  1:3287774/1‑71 (MQ=255)
                gagaGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCg                                                   >  1:3612611/1‑71 (MQ=255)
                gagaGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGACGTTCGACGTAGCTCTGCGTCGCTTCAAGCg                                                   >  1:625022/1‑71 (MQ=255)
                 agagGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGt                                                  >  1:3955954/1‑71 (MQ=255)
                 agagGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGt                                                  >  1:1975976/1‑71 (MQ=255)
                  gagGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGtt                                                 >  1:2975369/1‑71 (MQ=255)
                   agGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTc                                                >  1:1294462/1‑71 (MQ=255)
                    ggCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCg                                                               <  1:4207475/55‑1 (MQ=255)
                       acaTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGc                                            <  1:1018230/71‑1 (MQ=255)
                       acaTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGc                                            <  1:932962/71‑1 (MQ=255)
                         aTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGa                                          <  1:4697634/71‑1 (MQ=255)
                         aTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGa                                          <  1:2514086/71‑1 (MQ=255)
                         aTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGa                                          <  1:4650931/71‑1 (MQ=255)
                         aTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGa                                          <  1:1768505/71‑1 (MQ=255)
                          tGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGc                                                                >  1:2176984/1‑48 (MQ=255)
                          tGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGa                                          >  1:542875/1‑70 (MQ=255)
                           gCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaa                                         >  1:281357/1‑70 (MQ=255)
                            ccGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaaa                                       <  1:2203690/71‑1 (MQ=255)
                            ccGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa                                        >  1:1994760/1‑70 (MQ=255)
                            ccGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa                                        <  1:622552/70‑1 (MQ=255)
                            ccGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa                                        <  1:2549376/70‑1 (MQ=255)
                             cGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa                                        >  1:2831723/1‑69 (MQ=255)
                             cGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa                                        >  1:720970/1‑69 (MQ=255)
                                   ttAAAGTACGTGAAAATGAGCCGTTCGACGTAGCTCTGCGTc                                                             <  1:2218418/42‑1 (MQ=255)
                                   ttAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTc                                                             <  1:3634383/42‑1 (MQ=255)
                                   ttAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTc                                                             <  1:4754101/42‑1 (MQ=255)
                                   ttAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTc                                                             <  1:3722320/42‑1 (MQ=255)
                                   ttAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAgg                                 <  1:1864572/70‑1 (MQ=255)
                                   ttAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgt                                >  1:711071/1‑71 (MQ=255)
                                    tAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtg                               >  1:307214/1‑71 (MQ=255)
                                    tAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtg                               >  1:1850508/1‑71 (MQ=255)
                                     aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCaa                                                      >  1:435663/1‑47 (MQ=255)
                                     aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCaa                                                      >  1:3593242/1‑47 (MQ=255)
                                     aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt                              >  1:3040003/1‑71 (MQ=255)
                                     aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt                              >  1:364969/1‑71 (MQ=255)
                                     aaaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt                              >  1:4416356/1‑71 (MQ=255)
                                      aaGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt                              <  1:2317958/70‑1 (MQ=255)
                                           cGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGc                                    >  1:2404828/1‑59 (MQ=255)
                                           cGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGc                                    >  1:127102/1‑59 (MQ=255)
                                           cGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTgg                         <  1:1992416/70‑1 (MQ=255)
                                             tGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt                              <  1:1463528/63‑1 (MQ=255)
                                             tGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt                              <  1:2518852/63‑1 (MQ=255)
                                             tGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt                              <  1:880993/63‑1 (MQ=255)
                                              gAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCgg                      >  1:2811013/1‑70 (MQ=255)
                                               aaaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGgtgt                              <  1:2022568/61‑1 (MQ=255)
                                               aaaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCgg                      >  1:285253/1‑69 (MQ=255)
                                               aaaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaa                    >  1:1019933/1‑71 (MQ=255)
                                               aaaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaa                    >  1:1449614/1‑71 (MQ=255)
                                               aaaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGa                     >  1:432435/1‑70 (MQ=255)
                                                aaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCaa                                                      >  1:1226344/1‑36 (MQ=255)
                                                aaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCaa                                                      >  1:1408612/1‑36 (MQ=255)
                                                aaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCaa                                                      >  1:4305000/1‑36 (MQ=255)
                                                aaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCaa                                                      >  1:4679386/1‑36 (MQ=255)
                                                aaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaac                   <  1:1410787/71‑2 (MQ=255)
                                                aaaCGAGCCGTTCGAAGTCGCAATGCGTCGCTTCAAACGCACCATCGAAAAAACTGGTCTGCTGACGGaac                   <  1:3206717/71‑2 (MQ=255)
                                                 aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt                  <  1:4741940/71‑1 (MQ=255)
                                                 aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt                  <  1:987020/71‑1 (MQ=255)
                                                 aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt                  >  1:283093/1‑71 (MQ=255)
                                                 aaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt                  <  1:4660032/71‑1 (MQ=255)
                                                     aGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaaaa                                      >  1:217485/1‑47 (MQ=255)
                                                     aGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaaaa                                      >  1:4322648/1‑47 (MQ=255)
                                                         gTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc          <  1:1479754/71‑1 (MQ=255)
                                                         gTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc          <  1:1336043/71‑1 (MQ=255)
                                                          ttCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaa                                         <  1:4548268/39‑1 (MQ=255)
                                                            cGACGTAGCTCTGCGTCGCTTCAAGCTTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt        >  1:4333830/1‑70 (MQ=255)
                                                            cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTc                            >  1:375935/1‑50 (MQ=255)
                                                            cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa                    >  1:2890048/1‑58 (MQ=255)
                                                            cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa                    >  1:946537/1‑58 (MQ=255)
                                                            cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa                    >  1:544467/1‑58 (MQ=255)
                                                            cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGaa                    >  1:353370/1‑58 (MQ=255)
                                                            cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt        <  1:3036209/70‑1 (MQ=255)
                                                            cGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt        >  1:2437457/1‑70 (MQ=255)
                                                             gACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTg       <  1:4286524/70‑1 (MQ=255)
                                                                 tAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGtt                             <  1:2920429/44‑1 (MQ=255)
                                                                  aGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGt    >  1:3257521/1‑68 (MQ=255)
                                                                  aGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTc  >  1:1869581/1‑70 (MQ=255)
                                                                  |                                                                     
CGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTC  >  Exported/2061469‑2061604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: