| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,968,626 | A→G | 6.7% | S2P (TCC→CCC) | tktA ← | transketolase 1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,968,626 | 0 | A | G | 6.7% | 401.1 / 15.7 | 134 | S2P (TCC→CCC) | tktA | transketolase 1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base A (63/62); new base G (4/5); total (67/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
Only 100 of 134 total aligned reads displayed.
GATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTT > Exported/1968559‑1968689 | gATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTAC‑‑GAGGACAttt > 1:2967230/1‑71 (MQ=37) ttGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt > 1:3531816/1‑71 (MQ=255) ttGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt > 1:2489706/1‑71 (MQ=255) ttGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt > 1:154496/1‑71 (MQ=255) ttGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt > 1:572487/1‑71 (MQ=255) ttGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt > 1:4262856/1‑71 (MQ=255) ttGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt > 1:711201/1‑71 (MQ=255) ttGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt > 1:4248372/1‑71 (MQ=255) ttGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt > 1:811146/1‑71 (MQ=255) ttGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtt > 1:3684458/1‑69 (MQ=255) ttGGCTTTCTGTACTGCGTACATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAttt > 1:3451513/1‑70 (MQ=255) ttGGCTTTCTGCACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt > 1:4691776/1‑71 (MQ=255) tGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt < 1:2733097/70‑1 (MQ=255) ggCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTg < 1:4385204/70‑1 (MQ=255) ggCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTg < 1:3751829/70‑1 (MQ=255) ggCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGa > 1:3646760/1‑71 (MQ=255) ggCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGa > 1:4287994/1‑71 (MQ=255) ggCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGa > 1:1585285/1‑71 (MQ=255) gCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGAc < 1:2755512/71‑1 (MQ=255) gCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGAc < 1:3080984/71‑1 (MQ=255) gCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGAc < 1:799837/71‑1 (MQ=255) ttCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTc > 1:1614553/1‑70 (MQ=255) tGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACt > 1:1941592/1‑66 (MQ=255) tGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt < 1:455628/57‑1 (MQ=255) tGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt < 1:4550363/57‑1 (MQ=255) gCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTc > 1:3208152/1‑61 (MQ=255) cGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTc > 1:4474991/1‑60 (MQ=255) cGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTc > 1:3726828/1‑60 (MQ=255) cGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTc > 1:4479969/1‑60 (MQ=255) cGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTc > 1:1995757/1‑60 (MQ=255) cGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGgat < 1:3973860/70‑1 (MQ=255) cGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGgat < 1:4128141/70‑1 (MQ=255) cGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGgat < 1:4294856/70‑1 (MQ=255) gTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGgatga > 1:1923928/1‑71 (MQ=255) tCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGgatgat > 1:1081733/1‑71 (MQ=255) tCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGgat > 1:4028300/1‑68 (MQ=255) tCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGgat > 1:4059086/1‑68 (MQ=255) aTGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGGCAtttt < 1:1706445/50‑1 (MQ=255) aTGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt < 1:1278222/50‑1 (MQ=255) aTGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt < 1:3854970/50‑1 (MQ=255) aTGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt < 1:674255/50‑1 (MQ=255) aTGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt < 1:4325689/50‑1 (MQ=255) aTGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt < 1:2174618/50‑1 (MQ=255) tGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAg < 1:2813735/57‑1 (MQ=255) cTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAggg > 1:2865227/1‑71 (MQ=255) cTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAggg > 1:281613/1‑71 (MQ=255) cgcACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGc < 1:265137/71‑1 (MQ=255) cACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGgatgat < 1:149173/58‑1 (MQ=255) cACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGgatgat < 1:2312085/58‑1 (MQ=255) cACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGgatgat < 1:4626978/58‑1 (MQ=255) aCGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCt > 1:250295/1‑71 (MQ=255) cGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt < 1:485042/38‑1 (MQ=255) cGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt < 1:3368134/38‑1 (MQ=255) cGAATAGCATTGGCAAGCTCTTTACGTGAGGACAtttt < 1:3362612/38‑1 (MQ=255) cGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGAtgatga > 1:1182319/1‑58 (MQ=255) cGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCtt < 1:3585619/71‑1 (MQ=255) cGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCtt < 1:3778256/71‑1 (MQ=255) cGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCtt < 1:3903582/71‑1 (MQ=255) aaTAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTaa > 1:474951/1‑71 (MQ=255) aaTAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTaa > 1:389307/1‑71 (MQ=255) aTAGCATTGGCAAGCTCTTTACGTGAGGACAtttt > 1:3835747/1‑35 (MQ=255) aTAGCATTGGCAAGCTCTTTACGTGAGGACAtttt > 1:1248371/1‑35 (MQ=255) aTAGCATTGGCAAGCTCTTTACGTGAGGACAtttt > 1:1530152/1‑35 (MQ=255) aTAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAAc < 1:4246106/71‑1 (MQ=255) aTAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAAc < 1:96792/71‑1 (MQ=255) aTAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAAc < 1:4756319/71‑1 (MQ=255) aTAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAAc > 1:169834/1‑71 (MQ=255) tAGCATTGGCAAGCTCTTTACGTGAGGGCATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAAc > 1:952934/1‑70 (MQ=255) tAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAg < 1:568708/42‑1 (MQ=255) tAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAg < 1:2949929/42‑1 (MQ=255) tAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAggg > 1:545119/1‑58 (MQ=255) aTTGGCAAGCTCTTTACGTGAGGACGTTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACtt > 1:1423155/1‑71 (MQ=255) aTTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACtt < 1:4035588/71‑1 (MQ=255) aTTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACtt < 1:1506708/71‑1 (MQ=255) aTTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACtt < 1:258341/71‑1 (MQ=255) ttGGCAAGCTCTTTACGTGAGGGCATTTTGACTCCAGATCGgat < 1:4570584/44‑1 (MQ=255) ttGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGatgatg < 1:3903238/48‑1 (MQ=255) ttGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGatgatg < 1:3839208/48‑1 (MQ=255) ttGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGatgatg < 1:4598768/48‑1 (MQ=255) ggCAAGCTCTTTACGTGAGGGCATTTTGACTCCAGATCGgatga > 1:1006063/1‑44 (MQ=255) ggCAAGCTCTTTACGTGAGGGCATTTTGACTCCAGATCGgatga > 1:3438764/1‑44 (MQ=255) ggCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTaa > 1:3538208/1‑62 (MQ=255) ggCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTgac > 1:1257138/1‑71 (MQ=255) gCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTgac < 1:1188549/70‑1 (MQ=255) cAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTgacga < 1:2686952/71‑1 (MQ=255) cAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTgacga < 1:4164535/71‑1 (MQ=255) cAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTgacga < 1:3585436/71‑1 (MQ=255) gCTCTTTATGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAg > 1:2186796/1‑71 (MQ=255) gCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAg < 1:3977476/71‑1 (MQ=255) tctTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAgcg > 1:1010180/1‑71 (MQ=255) ctTTACGTGAGGACATTTTGACTCCAGATCGGATGATGaa < 1:798458/40‑1 (MQ=255) ctTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACa < 1:4388587/67‑1 (MQ=255) tttACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGc > 1:1010065/1‑47 (MQ=255) tttACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGc > 1:4449358/1‑47 (MQ=255) tACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGtt > 1:1590745/1‑71 (MQ=255) tACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGtt > 1:1372268/1‑71 (MQ=255) tACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGtt > 1:3141758/1‑71 (MQ=255) tACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGtt > 1:1834759/1‑71 (MQ=255) tGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTgac < 1:3677743/56‑1 (MQ=255) tGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTgac < 1:664241/56‑1 (MQ=255) | GATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAATAGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTT > Exported/1968559‑1968689 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |