| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,709,579 | A→G | 8.9% | V490A (GTC→GCC) | yphE ← | fused predicted sugar transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,709,579 | 0 | A | G | 8.9% | 261.8 / 14.4 | 90 | V490A (GTC→GCC) | yphE | fused predicted sugar transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base A (45/37); new base G (4/4); total (49/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
CATCTTCAGGTTCCTCGGTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTCACACACCAGCGGC > Exported/1709509‑1709647 | cATCTTCAGGTTCCTCGGTAAAATTCATCAGTGCACAGACAGAATGGTGGACATCAGCTCATCCACATTGa < 1:4688521/71‑1 (MQ=255) aTCTTCAGGTTCCTCGGTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATACACATTGAc > 1:1181945/1‑71 (MQ=255) ttCAGGTTCCTCGGTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCg > 1:3878263/1‑70 (MQ=255) tCAGGTTCCTCGGTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGAcc > 1:3253704/1‑68 (MQ=255) tCAGGTTCCTCGGTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCg > 1:489443/1‑69 (MQ=255) tCAGGTTCCTCGGTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCg > 1:280175/1‑69 (MQ=255) tCAGGTTCCTCGGTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCg > 1:4373231/1‑69 (MQ=255) cAGGTTCCTCGGTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCgg < 1:276527/69‑1 (MQ=255) aGGTTCCTCGGTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGc < 1:4441650/71‑1 (MQ=255) ggTTCCTCGGTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGGCCGGAGCg < 1:471866/71‑1 (MQ=255) ggTTCCTCGGTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGGCCGGAGCg < 1:3314010/71‑1 (MQ=255) gTTCCTCGGTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGt < 1:492490/71‑1 (MQ=255) gTTCCTCGGTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGt < 1:985857/71‑1 (MQ=255) tCCTCGGTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTg > 1:1842633/1‑70 (MQ=255) cTCGGTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGa < 1:3703793/69‑1 (MQ=255) ggTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGaaa > 1:4184949/1‑68 (MQ=255) ggTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGaaa > 1:665601/1‑68 (MQ=255) ggTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGaaa > 1:3536428/1‑68 (MQ=255) ggTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGaaa > 1:1754004/1‑68 (MQ=255) ggTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAAc < 1:469199/69‑1 (MQ=255) gTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTcc < 1:4369548/71‑1 (MQ=255) gTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTcc < 1:2578901/71‑1 (MQ=255) tAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCt > 1:4495509/1‑71 (MQ=255) tAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCt < 1:1181443/71‑1 (MQ=255) aaaTTCATCAGTGCACAGACAGAATGGCGGCCATCAGCTCATCCACATTGACCGGAGCGTGaaa > 1:2964022/1‑64 (MQ=255) aaaTTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGaaa > 1:3334566/1‑64 (MQ=255) aaaTTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGa < 1:968014/62‑1 (MQ=255) aaaTTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGa < 1:1491446/62‑1 (MQ=255) aaaTTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGa < 1:3587051/62‑1 (MQ=255) aaaTTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGa < 1:1706444/62‑1 (MQ=255) aaaTTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTg < 1:2520502/70‑1 (MQ=255) aaaTTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTg < 1:2621212/70‑1 (MQ=255) aaaTTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTg < 1:4136085/70‑1 (MQ=255) aTTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGaaa > 1:1179456/1‑62 (MQ=255) ttCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCgag < 1:2079729/71‑1 (MQ=255) ttCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCgag < 1:911019/71‑1 (MQ=255) tcatcaGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCgag > 1:285591/1‑70 (MQ=255) tcatcaGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCgag > 1:1718812/1‑70 (MQ=255) tcatcaGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCgag > 1:494123/1‑70 (MQ=255) tcatcaGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCgag > 1:203945/1‑70 (MQ=255) catcaGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCgaga < 1:76593/70‑1 (MQ=255) tcaGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGGGCGGAGCGTGAAACTCCTGCGAGAAcg < 1:2759839/71‑1 (MQ=255) gTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAAcgtgc < 1:4488842/71‑1 (MQ=255) gTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAAcgtgc < 1:1159432/71‑1 (MQ=255) acagacagAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAAcgtgccgtg < 1:4038097/71‑1 (MQ=255) agacagaATGGCGGACATCAGCTCATCCACATTGACCGGCGCGTGAAACTCCTGCGAGAACGAGCCGTGCt < 1:3767176/71‑1 (MQ=255) agacagaATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCt < 1:4580145/71‑1 (MQ=255) acagaATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTg > 1:763175/1‑70 (MQ=255) acagaATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTg > 1:4353860/1‑70 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGtaat < 1:2163531/71‑1 (MQ=255) gaATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGtaat < 1:3728546/71‑1 (MQ=255) aaTGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGtaata < 1:1200530/71‑1 (MQ=255) gCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAg > 1:15743/1‑71 (MQ=255) cGGACATCAGCTCCTCCACATTGACCGGAGCGTGAAACTCCTGCGAGaa > 1:279605/1‑49 (MQ=255) aCATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATg < 1:2273767/71‑1 (MQ=255) aGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGtaataa > 1:1786761/1‑59 (MQ=255) aGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGtaataa > 1:929115/1‑59 (MQ=255) aGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGtaataa > 1:1340495/1‑59 (MQ=255) aGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGt > 1:3477963/1‑70 (MQ=255) aGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGt > 1:3767651/1‑70 (MQ=255) aGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGt > 1:4130803/1‑70 (MQ=255) tCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATg < 1:4394954/63‑1 (MQ=255) cATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTcaca > 1:4265572/1‑70 (MQ=255) aTCCACATTGGCCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTcacaca > 1:2872676/1‑71 (MQ=255) aTCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTcacac < 1:4585434/70‑1 (MQ=255) aTCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTca < 1:2945649/67‑1 (MQ=255) aTCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTca < 1:1374482/67‑1 (MQ=255) aTCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTca < 1:2103905/67‑1 (MQ=255) tCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTCacacac > 1:1576263/1‑71 (MQ=255) ccACATTGGCCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGtaat < 1:1287506/50‑1 (MQ=255) ccACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCg > 1:4698417/1‑61 (MQ=255) ccACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCg > 1:4254957/1‑61 (MQ=255) ccACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCg > 1:4550857/1‑61 (MQ=255) ccACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCg > 1:1908589/1‑61 (MQ=255) ccACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTcaca > 1:927830/1‑67 (MQ=255) ccACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTcaca > 1:3144921/1‑67 (MQ=255) ccACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTcaca > 1:1299841/1‑67 (MQ=255) ccACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTcaca > 1:2969126/1‑67 (MQ=255) ccACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTcaca > 1:2915372/1‑67 (MQ=255) ccACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTCacacac > 1:4396689/1‑70 (MQ=255) cacaTTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTCacacac > 1:1175907/1‑69 (MQ=255) cacaTTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTCACACAcc < 1:3643730/70‑1 (MQ=255) cacaTTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTCACACAcc < 1:543579/70‑1 (MQ=255) cacaTTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTCACACAcc < 1:1658453/70‑1 (MQ=255) acaTTGGCCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGtaataa > 1:3294637/1‑50 (MQ=255) acaTTGGCCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGtaataa > 1:2050405/1‑50 (MQ=255) acaTTGGCCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGtaataa > 1:179989/1‑50 (MQ=255) gACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTCACACACCAGCGGc > 1:3466727/1‑70 (MQ=255) gACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTCACACACCAGCGGc < 1:3227488/70‑1 (MQ=255) gACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTCACACACCAGCGGc > 1:2959190/1‑70 (MQ=255) gACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTCACACACCAGCGGc > 1:2171317/1‑70 (MQ=255) | CATCTTCAGGTTCCTCGGTAAAATTCATCAGTGCACAGACAGAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTAATAACAGGATGCGGTCACACACCAGCGGC > Exported/1709509‑1709647 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |