Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,618,811 C→T 5.9% V321V (GTG→GTA eutI ← predicted phosphotransacetylase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,618,8110CT 5.9% 223.8 / 3.4 68V321V (GTG→GTAeutIpredicted phosphotransacetylase subunit
Reads supporting (aligned to +/- strand):  ref base C (26/38);  new base T (2/2);  total (28/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

CAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCAT  >  Exported/1618743‑1618876
                                                                    |                                                                 
cAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGca                                                                  >  1:3135263/1‑70 (MQ=255)
cAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGca                                                                  >  1:3196690/1‑70 (MQ=255)
 aGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGcaca                                                                <  1:1056692/71‑1 (MQ=255)
  gAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGcaca                                                                <  1:1363030/70‑1 (MQ=255)
        gACCTTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGc                                                           >  1:2101821/1‑69 (MQ=255)
        gACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGTACAGCTGcca                                                         >  1:3258520/1‑71 (MQ=255)
                  tcaACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAg                                               >  1:1771768/1‑71 (MQ=255)
                    aaCCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGc                                              <  1:983721/70‑1 (MQ=255)
                     aCCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGc                                              <  1:334114/69‑1 (MQ=255)
                     aCCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGc                                              <  1:2365024/69‑1 (MQ=255)
                     aCCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGc                                              <  1:3283737/69‑1 (MQ=255)
                     aCCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGc                                              <  1:3872819/69‑1 (MQ=255)
                     aCCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGc                                              <  1:4156462/69‑1 (MQ=255)
                     aCCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGc                                              <  1:4476684/69‑1 (MQ=255)
                     aCCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGc                                              <  1:1388478/69‑1 (MQ=255)
                     aCCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGc                                              <  1:696564/69‑1 (MQ=255)
                      ccAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACTCGGCACAg                                                               <  1:294841/51‑1 (MQ=255)
                       cAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGa                                          <  1:2519412/71‑1 (MQ=255)
                         gtgtTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATa                                        <  1:320015/71‑1 (MQ=255)
                         gtgtTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATa                                        <  1:459733/71‑1 (MQ=255)
                            tttGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAAtt                                     >  1:2086480/1‑71 (MQ=255)
                            tttGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAAt                                      <  1:2522461/70‑1 (MQ=255)
                             ttGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAAt                                      <  1:3010183/69‑1 (MQ=255)
                              tGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAAtt                                     >  1:1612759/1‑69 (MQ=255)
                              tGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTc                                   >  1:1421601/1‑71 (MQ=255)
                               gTAAACTGCTTTCGCTGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTc                                   <  1:2032417/70‑1 (MQ=255)
                                 aaaCTGCTTTCGCGGTTCACTTCTGTCTGACGCGGTACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTg                                >  1:218682/1‑71 (MQ=255)
                                  aaCTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCa                                                <  1:820206/54‑1 (MQ=255)
                                  aaCTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCa                                                <  1:2935987/54‑1 (MQ=255)
                                  aaCTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTg                                <  1:962463/70‑1 (MQ=255)
                                       cTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACg                            <  1:4394589/69‑1 (MQ=255)
                                       cTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACg                            <  1:760319/69‑1 (MQ=255)
                                                ttCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTc                                   <  1:924485/53‑1 (MQ=255)
                                                ttCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTc                                   <  1:710717/53‑1 (MQ=255)
                                                ttCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTc                                   <  1:546511/53‑1 (MQ=255)
                                                 tCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCAc                  >  1:2191353/1‑69 (MQ=255)
                                                 tCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCAc                  >  1:2528023/1‑69 (MQ=255)
                                                  cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCt                                             >  1:2694470/1‑41 (MQ=255)
                                                  cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCt                                 >  1:1082066/1‑53 (MQ=255)
                                                  cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCt                                 >  1:1621284/1‑53 (MQ=255)
                                                  cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCt                                 >  1:3404215/1‑53 (MQ=255)
                                                  cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCAc                  >  1:621399/1‑68 (MQ=255)
                                                  cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCAc                  >  1:3066517/1‑68 (MQ=255)
                                                  cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCAc                  >  1:3677731/1‑68 (MQ=255)
                                                  cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCAc                  >  1:3932733/1‑68 (MQ=255)
                                                  cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCAc                  >  1:4595599/1‑68 (MQ=255)
                                                  cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCAc                  >  1:3244327/1‑68 (MQ=255)
                                                    cTTCTGTCTGACGCGGTACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGc                           <  1:3585519/57‑1 (MQ=255)
                                                    cTTCTGTCTGACGCGGTACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGc                           <  1:529604/57‑1 (MQ=255)
                                                      tctgtctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgaga              >  1:2528731/1‑68 (MQ=255)
                                                       ctgtctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGa                                          >  1:722033/1‑39 (MQ=255)
                                                       ctgtctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagag             <  1:4157549/68‑1 (MQ=255)
                                                       ctgtctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagag             <  1:3626182/68‑1 (MQ=255)
                                                       ctgtctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgaga              >  1:4096422/1‑67 (MQ=255)
                                                          tctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagagaga          <  1:1987226/68‑1 (MQ=255)
                                                          tctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagagaga          <  1:4298482/68‑1 (MQ=255)
                                                          tctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagagaga          <  1:1148668/68‑1 (MQ=255)
                                                          tctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCg       <  1:417756/71‑1 (MQ=255)
                                                           ctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAAt                                      >  1:2518522/1‑39 (MQ=255)
                                                              aCGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCAc                  >  1:1888124/1‑56 (MQ=255)
                                                               cgcgGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCAt  <  1:72991/71‑1 (MQ=255)
                                                                 cgGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGt      <  1:4670102/65‑1 (MQ=255)
                                                                 cgGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGt      <  1:4622097/65‑1 (MQ=255)
                                                                 cgGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGt      <  1:1841501/65‑1 (MQ=255)
                                                                 cgGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGt      <  1:4280319/65‑1 (MQ=255)
                                                                   gCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCAc                             <  1:2772969/40‑1 (MQ=255)
                                                                   gCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCAc                             <  1:3136189/40‑1 (MQ=255)
                                                                   gCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCAc                             <  1:2729075/40‑1 (MQ=255)
                                                                   gCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagagaga          >  1:1373743/1‑59 (MQ=255)
                                                                   gCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagagaga          >  1:2681739/1‑59 (MQ=255)
                                                                   gCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagagaga          >  1:2587891/1‑59 (MQ=255)
                                                                   gCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagagaga          >  1:4151984/1‑59 (MQ=255)
                                                                    |                                                                 
CAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCAT  >  Exported/1618743‑1618876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: