Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 1,618,811 | C→T | 5.9% | V321V (GTG→GTA) | eutI ← | predicted phosphotransacetylase subunit |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 1,618,811 | 0 | C | T | 5.9% | 223.8 / 3.4 | 68 | V321V (GTG→GTA) | eutI | predicted phosphotransacetylase subunit |
Reads supporting (aligned to +/- strand): ref base C (26/38); new base T (2/2); total (28/40) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 |
CAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCAT > Exported/1618743‑1618876 | cAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGca > 1:3135263/1‑70 (MQ=255) cAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGca > 1:3196690/1‑70 (MQ=255) aGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGcaca < 1:1056692/71‑1 (MQ=255) gAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGcaca < 1:1363030/70‑1 (MQ=255) gACCTTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGc > 1:2101821/1‑69 (MQ=255) gACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGTACAGCTGcca > 1:3258520/1‑71 (MQ=255) tcaACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAg > 1:1771768/1‑71 (MQ=255) aaCCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGc < 1:983721/70‑1 (MQ=255) aCCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGc < 1:334114/69‑1 (MQ=255) aCCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGc < 1:2365024/69‑1 (MQ=255) aCCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGc < 1:3283737/69‑1 (MQ=255) aCCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGc < 1:3872819/69‑1 (MQ=255) aCCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGc < 1:4156462/69‑1 (MQ=255) aCCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGc < 1:4476684/69‑1 (MQ=255) aCCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGc < 1:1388478/69‑1 (MQ=255) aCCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGc < 1:696564/69‑1 (MQ=255) ccAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACTCGGCACAg < 1:294841/51‑1 (MQ=255) cAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGa < 1:2519412/71‑1 (MQ=255) gtgtTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATa < 1:320015/71‑1 (MQ=255) gtgtTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATa < 1:459733/71‑1 (MQ=255) tttGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAAtt > 1:2086480/1‑71 (MQ=255) tttGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAAt < 1:2522461/70‑1 (MQ=255) ttGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAAt < 1:3010183/69‑1 (MQ=255) tGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAAtt > 1:1612759/1‑69 (MQ=255) tGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTc > 1:1421601/1‑71 (MQ=255) gTAAACTGCTTTCGCTGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTc < 1:2032417/70‑1 (MQ=255) aaaCTGCTTTCGCGGTTCACTTCTGTCTGACGCGGTACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTg > 1:218682/1‑71 (MQ=255) aaCTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCa < 1:820206/54‑1 (MQ=255) aaCTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCa < 1:2935987/54‑1 (MQ=255) aaCTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTg < 1:962463/70‑1 (MQ=255) cTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACg < 1:4394589/69‑1 (MQ=255) cTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACg < 1:760319/69‑1 (MQ=255) ttCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTc < 1:924485/53‑1 (MQ=255) ttCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTc < 1:710717/53‑1 (MQ=255) ttCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTc < 1:546511/53‑1 (MQ=255) tCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCAc > 1:2191353/1‑69 (MQ=255) tCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCAc > 1:2528023/1‑69 (MQ=255) cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCt > 1:2694470/1‑41 (MQ=255) cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCt > 1:1082066/1‑53 (MQ=255) cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCt > 1:1621284/1‑53 (MQ=255) cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCt > 1:3404215/1‑53 (MQ=255) cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCAc > 1:621399/1‑68 (MQ=255) cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCAc > 1:3066517/1‑68 (MQ=255) cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCAc > 1:3677731/1‑68 (MQ=255) cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCAc > 1:3932733/1‑68 (MQ=255) cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCAc > 1:4595599/1‑68 (MQ=255) cACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCAc > 1:3244327/1‑68 (MQ=255) cTTCTGTCTGACGCGGTACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGc < 1:3585519/57‑1 (MQ=255) cTTCTGTCTGACGCGGTACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGc < 1:529604/57‑1 (MQ=255) tctgtctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgaga > 1:2528731/1‑68 (MQ=255) ctgtctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGa > 1:722033/1‑39 (MQ=255) ctgtctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagag < 1:4157549/68‑1 (MQ=255) ctgtctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagag < 1:3626182/68‑1 (MQ=255) ctgtctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgaga > 1:4096422/1‑67 (MQ=255) tctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagagaga < 1:1987226/68‑1 (MQ=255) tctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagagaga < 1:4298482/68‑1 (MQ=255) tctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagagaga < 1:1148668/68‑1 (MQ=255) tctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCg < 1:417756/71‑1 (MQ=255) ctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAAt > 1:2518522/1‑39 (MQ=255) aCGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCAc > 1:1888124/1‑56 (MQ=255) cgcgGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCAt < 1:72991/71‑1 (MQ=255) cgGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGt < 1:4670102/65‑1 (MQ=255) cgGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGt < 1:4622097/65‑1 (MQ=255) cgGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGt < 1:1841501/65‑1 (MQ=255) cgGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGt < 1:4280319/65‑1 (MQ=255) gCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCAc < 1:2772969/40‑1 (MQ=255) gCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCAc < 1:3136189/40‑1 (MQ=255) gCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCAc < 1:2729075/40‑1 (MQ=255) gCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagagaga > 1:1373743/1‑59 (MQ=255) gCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagagaga > 1:2681739/1‑59 (MQ=255) gCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagagaga > 1:2587891/1‑59 (MQ=255) gCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagagaga > 1:4151984/1‑59 (MQ=255) | CAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCAT > Exported/1618743‑1618876 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |