Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,283,882	T→C	11.0%	T1887T (ACT→ACC)	yeeJ →	adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,283,882	0	T	C	11.0%	233.0 / 17.7	82	T1887T (ACT→ACC)	yeeJ	adhesin
Reads supporting (aligned to +/- strand):  ref base T (42/31);  new base C (5/4);  total (47/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

CTATTGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGTCATCAACTTTAGCGTAACG  >  Exported/1283812‑1283951                                                                       |                                                                      cTATTGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTAct                                                                       <  1:2033060/71‑1 (MQ=255) cTATTGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTAct                                                                       <  1:400981/71‑1 (MQ=255) cTATTGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTAct                                                                       <  1:2438314/71‑1 (MQ=255) cTATTGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTAct                                                                       <  1:1194832/71‑1 (MQ=255)    ttGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTActct                                                                     <  1:4631698/70‑1 (MQ=255)    ttGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTActct                                                                     >  1:2445595/1‑70 (MQ=255)    ttGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTActct                                                                     <  1:1412022/70‑1 (MQ=255)    ttGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTActct                                                                     >  1:3632843/1‑70 (MQ=255)    ttGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTg                                                                    <  1:1316541/71‑1 (MQ=255)    ttGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTg                                                                    <  1:1929417/71‑1 (MQ=255)    ttGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTg                                                                    <  1:3619634/71‑1 (MQ=255)      gatgaAAAACTGACACTCACGTCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGAc                                                                  >  1:549687/1‑71 (MQ=255)      gatgaAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGa                                                                   <  1:4393895/70‑1 (MQ=255)      gatgaAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGAc                                                                  >  1:4406000/1‑71 (MQ=255)      gatgaAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGAc                                                                  >  1:737368/1‑71 (MQ=255)          aaaaaCTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGAc                                                                  >  1:4276232/1‑67 (MQ=255)           aaaaCTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCaa                                                             >  1:143629/1‑71 (MQ=255)               cTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCt                                                         <  1:4310448/71‑1 (MQ=255)                tGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACgg                                                                >  1:4724839/1‑63 (MQ=255)                tGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACgg                                                                >  1:1339398/1‑63 (MQ=255)                tGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACgg                                                                >  1:3365739/1‑63 (MQ=255)                tGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACgg                                                                >  1:3859258/1‑63 (MQ=255)                  acacTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTATAGGCGCTACTCTGACGGCAACGCTAAc                                                      <  1:997965/71‑1 (MQ=255)                  acacTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAAc                                                      >  1:4082476/1‑71 (MQ=255)                     cTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACctc                                                   <  1:4495654/71‑1 (MQ=255)                     cTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACCCTGACGGCAACGCTAACctc                                                   >  1:4740884/1‑71 (MQ=255)                     cTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACCCTGACGGCAACGCTAACctc                                                   >  1:3595065/1‑71 (MQ=255)                     cTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACCCTGACGGCAACGCTAACctc                                                   >  1:3594518/1‑71 (MQ=255)                     cTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACCCTGACGGCAACGCTAACctc                                                   >  1:4229589/1‑71 (MQ=255)                     cTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACCCTGACGGCAAAGCTAACctc                                                   >  1:2860524/1‑71 (MQ=255)                      tCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTActct                                                                     <  1:1692583/52‑1 (MQ=255)                      tCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTActct                                                                     <  1:2604485/52‑1 (MQ=255)                       cACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTg                                                 >  1:2929537/1‑71 (MQ=255)                        aCGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTg                                                 >  1:2753494/1‑70 (MQ=255)                        aCGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTg                                                 >  1:3703357/1‑70 (MQ=255)                        aCGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTg                                                 >  1:3653282/1‑70 (MQ=255)                        aCGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTg                                                 >  1:1721400/1‑70 (MQ=255)                        aCGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTg                                                 >  1:1384904/1‑70 (MQ=255)                         cGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCa                                               <  1:3645576/71‑1 (MQ=255)                         cGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCa                                               <  1:1629663/71‑1 (MQ=255)                          ggCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCaa                                              <  1:3376406/71‑1 (MQ=255)                          ggCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCaa                                              <  1:3491883/71‑1 (MQ=255)                          ggCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCaa                                              <  1:2322704/71‑1 (MQ=255)                          ggCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCaa                                              <  1:4355624/71‑1 (MQ=255)                          ggCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCaa                                              <  1:966640/71‑1 (MQ=255)                          ggCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCa                                               <  1:460261/70‑1 (MQ=255)                          ggCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACCCTGACGGCAACGCTAACCTCTGCaa                                              <  1:3681024/71‑1 (MQ=255)                          ggCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACCCTGACGGCAACGCTAACCTCTGCaa                                              <  1:2143833/71‑1 (MQ=255)                          ggCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACCCTGACGGCAACGCTAACCTCTGCaa                                              <  1:1183242/71‑1 (MQ=255)                          ggCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACCCTGACGGCAACGCTAACCTCTGCaa                                              <  1:79499/71‑1 (MQ=255)                              tCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATgg                                          <  1:1999614/71‑1 (MQ=255)                              tCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATgg                                          <  1:48647/71‑1 (MQ=255)                              tCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATgg                                          <  1:3341663/71‑1 (MQ=255)                               ccAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAAc                                                      <  1:1582173/58‑1 (MQ=255)                                cAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCa                                        >  1:1609094/1‑71 (MQ=255)                                 aGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACct                                                    >  1:3220562/1‑58 (MQ=255)                                 aGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCAc                                       <  1:4365894/71‑1 (MQ=255)                                  gTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTg                                                                    <  1:200193/41‑1 (MQ=255)                                          aTCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTTCAGTgg                               <  1:11036/70‑1 (MQ=255)                                          aTCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTg                                >  1:2736999/1‑69 (MQ=255)                                          aTCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTg                                >  1:2027126/1‑69 (MQ=255)                                           tCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGa                              <  1:588866/70‑1 (MQ=255)                                           tCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTATGCAAATGGCACTCCAGTGGAg                             >  1:1679343/1‑71 (MQ=255)                                             ggTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACct                                                    >  1:4742085/1‑46 (MQ=255)                                             ggTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACct                                                    >  1:3902876/1‑46 (MQ=255)                                              gtgtCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGggt                          <  1:955155/71‑1 (MQ=255)                                               tgtCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGggtc                         <  1:1040225/71‑1 (MQ=255)                                                 tCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTa                                                        <  1:1196269/38‑1 (MQ=255)                                                 tCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTgg                               <  1:1728492/63‑1 (MQ=255)                                                 tCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGggtcag                       >  1:3296253/1‑71 (MQ=255)                                                   tATGCCCCTACAGGCGCTACTCTGACGGCAACGCTaa                                                       >  1:2164905/1‑37 (MQ=255)                                                   tATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATg                                           >  1:3319623/1‑49 (MQ=255)                                                   tATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATg                                           >  1:2872787/1‑49 (MQ=255)                                                    aTGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGggtcagg                      >  1:3217594/1‑69 (MQ=255)                                                       ccccTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCACTGGAGGGTCAGGtcat                  >  1:162761/1‑70 (MQ=255)                                                         ccTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGTCATCaa               >  1:1812038/1‑71 (MQ=255)                                                         ccTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGTCATCaa               >  1:1563403/1‑71 (MQ=255)                                                           tACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATgg                                          <  1:1793387/42‑1 (MQ=255)                                                             cAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGTCATCAACtt            <  1:4231300/70‑1 (MQ=255)                                                             cAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGGCAGGTCATCAACttt           <  1:2324352/71‑1 (MQ=255)                                                               ggCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGTCATCAACTTTAg         >  1:466299/1‑71 (MQ=255)                                                                 cgcTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGtcatcagc              >  1:1149075/1‑62 (MQ=255)                                                                 cgcTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGTCATCaa               >  1:217929/1‑63 (MQ=255)                                                                 cgcTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGTCATCAAc              >  1:4378605/1‑64 (MQ=255)                                                                 cgcTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGTCATCAAc              >  1:1170104/1‑64 (MQ=255)                                                                   cTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGTCATCAACTTTAGCGt      <  1:416513/70‑1 (MQ=255)                                                                     aCTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAg                                  >  1:1897287/1‑40 (MQ=255)                                                                     aCTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAg                                  >  1:591088/1‑40 (MQ=255)                                                                     aCTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAg                                  >  1:1503577/1‑40 (MQ=255)                                                                     aCTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAg                                  >  1:4584822/1‑40 (MQ=255)                                                                     aCTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGTCATCAACTTTAGCGTaa    >  1:2433074/1‑70 (MQ=255)                                                                     aCTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGTCATCAACTTTAGCGTaa    >  1:4557766/1‑70 (MQ=255)                                                                      ctctGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGTCATCAACTTTAGCGTaa    >  1:3446765/1‑69 (MQ=255)                                                                      ctctGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGTCATCAACTTTAGCGTAACg  >  1:2678165/1‑71 (MQ=255)                                                                      ctctGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGTCATCAACTTTAGCGTAACg  >  1:1541320/1‑71 (MQ=255)                                                                      ctctGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGTCATCAACTTTAGCGTAACg  >  1:1786421/1‑71 (MQ=255)                                                                       |                                                                      CTATTGATGAAAAACTGACACTCACGGCGTCCAGTCCGCTTATCGGTGTCTATGCCCCTACAGGCGCTACTCTGACGGCAACGCTAACCTCTGCAAATGGCACTCCAGTGGAGGGTCAGGTCATCAACTTTAGCGTAACG  >  Exported/1283812‑1283951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑