Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,225,925 T→C 8.6% L77P (CTT→CCT)  yebV → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,225,9250TC 8.6% 236.6 / 11.0 81L77P (CTT→CCT) yebVhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (36/38);  new base C (4/3);  total (40/41)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTAAATGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTCCCCCGTTGTAAAAT  >  Exported/1225855‑1225993
                                                                      |                                                                    
cTAAATGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCt                                                                      >  1:4681680/1‑71 (MQ=255)
  aaaTGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTg                                                                    >  1:350664/1‑71 (MQ=255)
  aaaTGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTg                                                                    >  1:1726743/1‑71 (MQ=255)
   aaTGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCtt                                                                     <  1:4217927/69‑1 (MQ=255)
     tGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCt                                                                 <  1:3712900/71‑1 (MQ=255)
          aaCACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCTGATc                                                             >  1:2770184/1‑70 (MQ=255)
          aaCACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCTGATc                                                             >  1:2650761/1‑70 (MQ=255)
           acacTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATc                                                             >  1:1090737/1‑69 (MQ=255)
            cacTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCTGATCCaa                                                          <  1:4340774/71‑1 (MQ=255)
                   tCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAg                                                      <  1:4314006/68‑1 (MQ=255)
                   tCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAg                                                      <  1:3220306/68‑1 (MQ=255)
                    ccTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAAcc                                                  >  1:893098/1‑71 (MQ=255)
                       ttACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGt                                               >  1:23857/1‑71 (MQ=255)
                       ttACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGt                                               >  1:1978566/1‑71 (MQ=255)
                        tACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCg                                                <  1:2828007/69‑1 (MQ=255)
                        tACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCTGATCCAAAAAGAACCCg                                                <  1:3013384/69‑1 (MQ=255)
                         aCCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTc                                              <  1:2427829/70‑1 (MQ=255)
                         aCCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTc                                              <  1:1768942/70‑1 (MQ=255)
                         aCCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTc                                              <  1:4623815/70‑1 (MQ=255)
                           cGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa                                                         <  1:3155362/57‑1 (MQ=255)
                           cGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa                                                         <  1:2126508/57‑1 (MQ=255)
                           cGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCgg                                            >  1:4183816/1‑70 (MQ=255)
                           cGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCgg                                            >  1:2784084/1‑70 (MQ=255)
                                  gTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAAc                                                   >  1:2503029/1‑56 (MQ=255)
                                   ttACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCgg                                            <  1:4246453/62‑1 (MQ=255)
                                   ttACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCgg                                            <  1:883338/62‑1 (MQ=255)
                                   ttACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCgg                                            <  1:4135382/62‑1 (MQ=255)
                                    tACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGaa                                                    >  1:1859110/1‑53 (MQ=255)
                                    tACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGaa                                                    >  1:1583611/1‑53 (MQ=255)
                                    tACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGt                                  >  1:4271455/1‑71 (MQ=255)
                                    tACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGt                                  >  1:4162887/1‑71 (MQ=255)
                                     aCGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCa                                          >  1:154467/1‑62 (MQ=255)
                                     aCGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCa                                          >  1:736866/1‑62 (MQ=255)
                                     aCGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCa                                          >  1:2088951/1‑62 (MQ=255)
                                     aCGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCa                                          >  1:1725813/1‑62 (MQ=255)
                                     aCGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGt                                  >  1:1164711/1‑70 (MQ=255)
                                     aCGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGt                                  >  1:1367280/1‑70 (MQ=255)
                                     aCGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGt                                  >  1:2953383/1‑70 (MQ=255)
                                      cGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTa                                <  1:2153486/71‑1 (MQ=255)
                                      cGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTa                                <  1:1357825/71‑1 (MQ=255)
                                      cGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTa                                <  1:4108847/71‑1 (MQ=255)
                                       gATCAAGAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTAt                               <  1:493320/71‑1 (MQ=255)
                                       gATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTAt                               <  1:3786325/71‑1 (MQ=255)
                                       gATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTAt                               <  1:169019/71‑1 (MQ=255)
                                       gATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTAt                               <  1:3441284/71‑1 (MQ=255)
                                         tCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCa                                          >  1:1748508/1‑58 (MQ=255)
                                         tCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTAttt                             >  1:113249/1‑71 (MQ=255)
                                         tCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTAttt                             >  1:752616/1‑71 (MQ=255)
                                          caacaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa                                                         <  1:4022239/42‑1 (MQ=255)
                                          caacaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTg                            >  1:4633650/1‑71 (MQ=255)
                                          caacaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTg                            >  1:3719460/1‑71 (MQ=255)
                                           aacaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa                                                         <  1:3589597/41‑1 (MQ=255)
                                           aacaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa                                                         <  1:4146275/41‑1 (MQ=255)
                                           aacaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa                                                         <  1:3094818/41‑1 (MQ=255)
                                           aacaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa                                                         <  1:814675/41‑1 (MQ=255)
                                           aacaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAAc                                                   >  1:278059/1‑47 (MQ=255)
                                            acaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa                                                         <  1:2088513/40‑1 (MQ=255)
                                            acaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTc                          <  1:1563236/71‑1 (MQ=255)
                                             caaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTc                          <  1:2666349/70‑1 (MQ=255)
                                               aTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa                                                         <  1:2855339/37‑1 (MQ=255)
                                               aTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa                                                         <  1:4377854/37‑1 (MQ=255)
                                               aTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa                                                         <  1:3110544/37‑1 (MQ=255)
                                               aTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa                                                         <  1:160747/37‑1 (MQ=255)
                                               aTCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa                                                         <  1:2061519/37‑1 (MQ=255)
                                               aTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTg                       >  1:3381899/1‑71 (MQ=255)
                                               aTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTg                       >  1:1676546/1‑71 (MQ=255)
                                                tCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa                                                         <  1:896423/36‑1 (MQ=255)
                                                    aTGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGc                                      >  1:1940963/1‑51 (MQ=255)
                                                      gCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTc                >  1:4299981/1‑71 (MQ=255)
                                                      gCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTc                >  1:2308470/1‑71 (MQ=255)
                                                      gCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTc                >  1:2241432/1‑71 (MQ=255)
                                                        cTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTccc              <  1:4268722/71‑1 (MQ=255)
                                                         tGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGt                     <  1:2262931/63‑1 (MQ=255)
                                                         tGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGt                     <  1:2332397/63‑1 (MQ=255)
                                                         tGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGt                     <  1:766644/63‑1 (MQ=255)
                                                           gATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCa                                          >  1:2988299/1‑40 (MQ=255)
                                                           gATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCa                                          >  1:500140/1‑40 (MQ=255)
                                                           gATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCa                                          >  1:107967/1‑40 (MQ=255)
                                                           gATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTCCCCCg           >  1:1201416/1‑71 (MQ=255)
                                                           gATTATGCGTCTTGCCTGATACAAAAAGAACCCGTCGGCa                                          >  1:2708150/1‑40 (MQ=255)
                                                                 gCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTCCCCCGTTGTa      >  1:196018/1‑70 (MQ=255)
                                                                    tCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTCCCCCGTTGTAAAAt  <  1:490140/71‑1 (MQ=255)
                                                                     cTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTa                                <  1:1377929/40‑1 (MQ=255)
                                                                      ttGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTAtt                              >  1:1538673/1‑41 (MQ=255)
                                                                      |                                                                    
CTAAATGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTCCCCCGTTGTAAAAT  >  Exported/1225855‑1225993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: