Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 874,334 T→C 6.3% pseudogene (221/299 nt)
pseudogene (111/189 nt)
yciX →
yciX →
ECK1270:JW5198+JW5199:b4574; hypothetical protein
ECK1270:JW5199:b4523; hypothetical protein, C‑ter fragment

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported874,3340TC 6.3% 336.9 / 9.3 111pseudogene (221/299 nt)
pseudogene (111/189 nt)
yciX
yciX
ECK1270:JW5198+JW5199:b4574; hypothetical protein
ECK1270:JW5199:b4523; hypothetical protein, C‑ter fragment
Reads supporting (aligned to +/- strand):  ref base T (54/50);  new base C (5/2);  total (59/52)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

GGAGTCGTCACCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGATCAGCCG  >  Exported/874265‑874403
                                                                     |                                                                     
 gAGTCGTCACCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTc                                                                      <  1:315549/70‑1 (MQ=255)
 gAGTCGTCACCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTc                                                                      <  1:4665504/70‑1 (MQ=255)
      gtcACCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAga                                                                  >  1:2129278/1‑69 (MQ=255)
         aCCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACt                                                             >  1:2967585/1‑71 (MQ=255)
         aCCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACt                                                             >  1:3772954/1‑71 (MQ=255)
         aCCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACt                                                             >  1:3707111/1‑71 (MQ=255)
         aCCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACt                                                             >  1:3464918/1‑71 (MQ=255)
         aCCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACt                                                             >  1:4645889/1‑71 (MQ=255)
            aTTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGc                                                          <  1:2812646/71‑1 (MQ=255)
            aTTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGc                                                          <  1:1725022/71‑1 (MQ=255)
             ttATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGc                                                          >  1:835867/1‑70 (MQ=255)
               aTGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATCCCAGACGACTGGCAAc                                                       >  1:312060/1‑71 (MQ=255)
               aTGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATCCCAGACGACTGGCAAc                                                       >  1:3757491/1‑71 (MQ=255)
                 gCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACt                                                      >  1:2522413/1‑70 (MQ=255)
                 gCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACt                                                      >  1:358327/1‑70 (MQ=255)
                  ccAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTaa                                                    <  1:4345783/71‑1 (MQ=255)
                   cAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAAc                                                   <  1:727790/71‑1 (MQ=255)
                       aTAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCg                                               >  1:3226517/1‑71 (MQ=255)
                        tAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgc                                              <  1:2891336/71‑1 (MQ=255)
                        tAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgc                                              <  1:756413/71‑1 (MQ=255)
                          gTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcag                                            >  1:4009613/1‑71 (MQ=255)
                          gTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcag                                            >  1:2983343/1‑71 (MQ=255)
                          gTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcag                                            >  1:918459/1‑71 (MQ=255)
                          gTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcag                                            >  1:976774/1‑71 (MQ=255)
                           tGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTAtt                                                                       <  1:17141/43‑1 (MQ=255)
                           tGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTAtt                                                                       <  1:4153159/43‑1 (MQ=255)
                           tGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTAtt                                                                       <  1:2870569/43‑1 (MQ=255)
                           tGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcagc                                           <  1:2041895/71‑1 (MQ=255)
                           tGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcagc                                           <  1:1698280/71‑1 (MQ=255)
                           tGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcagc                                           <  1:2515893/71‑1 (MQ=255)
                             aCAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcagcag                                         <  1:3415987/71‑1 (MQ=255)
                             aCAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcagca                                          <  1:721245/70‑1 (MQ=255)
                             aCAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcagca                                          <  1:2968003/70‑1 (MQ=255)
                              cAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcagcagc                                        <  1:4123295/71‑1 (MQ=255)
                              cAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcagca                                          <  1:4538716/69‑1 (MQ=255)
                               aaTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCa                                                                    >  1:4286111/1‑42 (MQ=255)
                               aaTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCa                                                                    >  1:1038758/1‑42 (MQ=255)
                               aaTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCa                                                                    >  1:1873547/1‑42 (MQ=255)
                               aaTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCa                                                                    >  1:2890498/1‑42 (MQ=255)
                                aTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTc                                                                      <  1:2694930/39‑1 (MQ=255)
                                aTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTc                                                                      <  1:4498264/39‑1 (MQ=255)
                                aTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTc                                                                      <  1:3072250/39‑1 (MQ=255)
                                aTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTc                                                                      <  1:1562322/39‑1 (MQ=255)
                                aTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTc                                                                      <  1:973019/39‑1 (MQ=255)
                                aTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCaa                                      <  1:3744177/71‑1 (MQ=255)
                                aTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCaa                                      <  1:2786436/71‑1 (MQ=255)
                                aTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCaa                                      <  1:4454520/71‑1 (MQ=255)
                                aTGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCaa                                      <  1:2182049/71‑1 (MQ=255)
                                 tGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAg                                     <  1:564856/71‑1 (MQ=255)
                                  gAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCaa                                      >  1:4330664/1‑69 (MQ=255)
                                   aaaCAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGc                                    <  1:3955953/70‑1 (MQ=255)
                                   aaaCAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGc                                    <  1:1703405/70‑1 (MQ=255)
                                    aaCAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAgacgac                                                              >  1:1201769/1‑43 (MQ=255)
                                    aaCAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAgacgac                                                              >  1:982151/1‑43 (MQ=255)
                                    aaCAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAgacgac                                                              >  1:3651101/1‑43 (MQ=255)
                                    aaCAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAgacgac                                                              >  1:1074911/1‑43 (MQ=255)
                                       aGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCgcagc                                           <  1:4518048/59‑1 (MQ=255)
                                       aGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTta                               <  1:935600/71‑1 (MQ=255)
                                          aacgaaacgGGAATGTTCCGTCGTTATCCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAg                            <  1:1717726/71‑1 (MQ=255)
                                           acgaaacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAAc                                                   >  1:306858/1‑47 (MQ=255)
                                           acgaaacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAAc                                                   >  1:2505157/1‑47 (MQ=255)
                                           acgaaacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCaa                                      <  1:541357/60‑1 (MQ=255)
                                            cgaaacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGaa                          >  1:3736394/1‑71 (MQ=255)
                                            cgaaacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGaa                          >  1:1468818/1‑71 (MQ=255)
                                            cgaaacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGaa                          >  1:3213927/1‑71 (MQ=255)
                                              aaacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCaa                                                        >  1:2618660/1‑39 (MQ=255)
                                               aacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTg                       <  1:4723008/71‑1 (MQ=255)
                                               aacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTg                       >  1:4330163/1‑71 (MQ=255)
                                               aacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTg                       >  1:4221391/1‑71 (MQ=255)
                                               aacgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTg                       >  1:1529822/1‑71 (MQ=255)
                                                acgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGt                      >  1:4338402/1‑71 (MQ=255)
                                                 cgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATc                                                <  1:2625307/44‑1 (MQ=255)
                                                 cgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATc                                                <  1:917619/44‑1 (MQ=255)
                                                 cgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGtt                     <  1:650150/71‑1 (MQ=255)
                                                 cgGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGtt                     <  1:859571/71‑1 (MQ=255)
                                                  gggAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGttt                    >  1:4544126/1‑71 (MQ=255)
                                                  gggAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGtt                     <  1:364867/70‑1 (MQ=255)
                                                      aTGTTCCTTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGcc                                   >  1:1135968/1‑52 (MQ=255)
                                                      aTGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGcc                                   >  1:4452701/1‑52 (MQ=255)
                                                      aTGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGcc                                   >  1:305805/1‑52 (MQ=255)
                                                      aTGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGcc                                   >  1:1034512/1‑52 (MQ=255)
                                                         ttCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTg                >  1:1552331/1‑68 (MQ=255)
                                                         ttCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTg                >  1:2135540/1‑68 (MQ=255)
                                                         ttCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTg                >  1:1469150/1‑68 (MQ=255)
                                                         ttCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAg             <  1:1559934/71‑1 (MQ=255)
                                                         ttCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAg             <  1:2844832/71‑1 (MQ=255)
                                                         ttCCGTCGTTATCCCAGACGACTGGCAACTAACATCGCAGCAGCAAGcc                                   >  1:4577731/1‑49 (MQ=255)
                                                         ttCCGTCGTTATCCCAGACGACTGGCAACTAACATCGCAGCAGCAAGcc                                   >  1:2446936/1‑49 (MQ=255)
                                                            cgtcgtTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAgatg          <  1:4014256/71‑1 (MQ=255)
                                                             gtcgtTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAgatga         <  1:1364006/71‑1 (MQ=255)
                                                             gtcgtTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAgatga         <  1:1331582/71‑1 (MQ=255)
                                                              tcgtTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGtt                     <  1:4513450/58‑1 (MQ=255)
                                                               cgtTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAgatgat        >  1:3167857/1‑70 (MQ=255)
                                                                gtTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAgatgat        >  1:4582647/1‑69 (MQ=255)
                                                                gtTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAgatgat        >  1:1933518/1‑69 (MQ=255)
                                                                  tATCCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAg                            <  1:3166850/47‑1 (MQ=255)
                                                                   aTTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGATCAGcc   >  1:3593769/1‑71 (MQ=255)
                                                                   aTTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGATCAGc    >  1:2078117/1‑70 (MQ=255)
                                                                    ttCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGATCAGCCg  <  1:3762735/71‑1 (MQ=255)
                                                                     tCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGaa              <  1:786180/58‑1 (MQ=255)
                                                                     |                                                                     
GGAGTCGTCACCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTTCCGTCGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGATCAGCCG  >  Exported/874265‑874403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: