| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 750,056 | T→A | 9.7% | D1055V (GAT→GTT) | mfd ← | transcription‑repair coupling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 750,056 | 0 | T | A | 9.7% | 197.1 / 12.8 | 72 | D1055V (GAT→GTT) | mfd | transcription‑repair coupling factor |
| Reads supporting (aligned to +/- strand): ref base T (25/40); new base A (3/4); total (28/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
TCCTGATCCCCAGTTTCTGCGCTTGCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGC > Exported/749991‑750123 | tCCTGATCCCCAGTTTCTGCGCTTGCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTgg > 1:3830519/1‑70 (MQ=255)tCCTGATCCCCAGTTTCTGCGCTTGCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTgg > 1:158345/1‑70 (MQ=255) ccTGATCCCCAGTTTCTGCGCTTGCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTgg > 1:2206455/1‑69 (MQ=255) cTGATCCCCAGTTTCTGCGCTTGCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGgcag < 1:579851/71‑1 (MQ=255) aTCCCCAGTTTCTGCGCTTGCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGcagcag > 1:2493071/1‑71 (MQ=255) ccccAGTTTCTGCGCTTGCTGGCGCAGACGGGCAATATCCATCAGGGTACGCGCCGGATCTGGCAGCAGGc > 1:2833906/1‑71 (MQ=255) ccccAGTTTCTGCGCTTGCTGGCGCAGACGGGCAATATCCAGAAGGGTACGCGCCGGATCTGGCAGCAGGc > 1:4220747/1‑71 (MQ=255) tCTGCGCTTGCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGcg < 1:1086207/70‑1 (MQ=255) cTGCGCTTGCTGGCGCAGACGGGCAATATCCAGTAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGcgat > 1:4314337/1‑71 (MQ=255) cTGCGCTTGCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGcgat > 1:3497374/1‑71 (MQ=255) cTGCGCTTGCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGcgat > 1:1477742/1‑71 (MQ=255) gcTTGCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGcgatcgat < 1:4028773/71‑1 (MQ=255) cTTGCTGGCGCAGCCGGGCAATATCCAGCATGGTACGCGCC‑GATCTGGCAGCAGGCCGAAGcgatcgat > 1:1619424/1‑69 (MQ=255) cTTGCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGcgatcgat > 1:4290838/1‑70 (MQ=255) cTTGCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGcgatcgat > 1:4378522/1‑70 (MQ=255) atGCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGAACTGGCAGCAGGCCGAAGcgatcgat > 1:2040698/2‑69 (MQ=255) gCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGcgat < 1:2038703/63‑1 (MQ=255) gCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGcgat < 1:4004288/63‑1 (MQ=255) gCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGcgat < 1:4126279/63‑1 (MQ=255) gCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGcgat < 1:1219196/63‑1 (MQ=255) gCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCAGAAGcgat < 1:3560254/63‑1 (MQ=255) cTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGc < 1:1783273/70‑1 (MQ=255) cTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGc < 1:664650/70‑1 (MQ=255) cTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGc < 1:1368901/70‑1 (MQ=255) gcgcAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTc < 1:4608564/69‑1 (MQ=255) gcgcAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTc < 1:583222/69‑1 (MQ=255) gcgcAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTc < 1:4722884/69‑1 (MQ=255) gcgcAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGa > 1:4222683/1‑71 (MQ=255) gcAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTg > 1:3786614/1‑39 (MQ=255) gcAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTg > 1:3424846/1‑39 (MQ=255) gcAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACt < 1:1887862/71‑1 (MQ=255) gcAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACt < 1:2514594/71‑1 (MQ=255) gACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTg < 1:4720836/71‑1 (MQ=255) gACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTg < 1:1459396/71‑1 (MQ=255) aCGGGCAATATCCAGCAGGGTACGCGCCGGAACTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGa > 1:3134455/1‑71 (MQ=255) cGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGAt > 1:1429354/1‑71 (MQ=255) gggCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGcgatc > 1:1332964/1‑52 (MQ=255) gCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGAtctc < 1:898081/71‑1 (MQ=255) atatCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGc < 1:4365370/43‑1 (MQ=255) atatCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGc < 1:3350401/43‑1 (MQ=255) atatCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGc < 1:2359014/43‑1 (MQ=255) atatCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGc < 1:466646/43‑1 (MQ=255) tatCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAg < 1:777271/41‑1 (MQ=255) tatCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAg < 1:98425/41‑1 (MQ=255) tatCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAg < 1:2515413/41‑1 (MQ=255) tatCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAg < 1:3546940/41‑1 (MQ=255) tatCCAGCAGGGTACGCGCCGGAACTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTc < 1:165040/70‑1 (MQ=255) tatCCAGCAGGGTACGCGCCGGAACTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTc < 1:2984516/70‑1 (MQ=255) tatCCAGCAGGGTACGCGCCGGAACTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTc < 1:4249874/70‑1 (MQ=255) tatCCAGCAGGGTACGCGCCGGAACTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTc < 1:3547823/70‑1 (MQ=255) tCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAg > 1:32534/1‑71 (MQ=255) tCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAg > 1:1933175/1‑71 (MQ=255) tCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAg > 1:2756166/1‑71 (MQ=255) agcagGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAGTTc < 1:4291524/71‑1 (MQ=255) agcagGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAGTTc < 1:3749908/71‑1 (MQ=255) cagGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCg < 1:3174666/49‑1 (MQ=255) cagGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCg < 1:704056/49‑1 (MQ=255) cagGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTcc < 1:830527/64‑1 (MQ=255) cagGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTcc < 1:3961581/64‑1 (MQ=255) cagGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAGTTCGt < 1:3996345/71‑1 (MQ=255) gggTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAg > 1:4163947/1‑64 (MQ=255) gggTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAg > 1:211793/1‑64 (MQ=255) gggTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAg > 1:2610990/1‑64 (MQ=255) tACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTcc > 1:2717532/1‑71 (MQ=255) tACGCGCCGGAACTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCa > 1:3944606/1‑60 (MQ=255) cgcgCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAGTTCGtttt < 1:57344/67‑1 (MQ=255) cgcgCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGt < 1:3691290/71‑1 (MQ=255) gcgcCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGtt < 1:4577981/71‑1 (MQ=255) ggATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGgc < 1:4222019/71‑1 (MQ=255) ggATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGgc > 1:445598/1‑71 (MQ=255) ggATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGgc < 1:874376/71‑1 (MQ=255) ggATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGgc < 1:4216589/71‑1 (MQ=255) | TCCTGATCCCCAGTTTCTGCGCTTGCTGGCGCAGACGGGCAATATCCAGCAGGGTACGCGCCGGATCTGGCAGCAGGCCGAAGCGATCGATAAGCTCGACTTTGATCTCTTCCAGTTCGTTTTCCGTTTTGGC > Exported/749991‑750123 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |