Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 705,870 A→G 30.8% intergenic (‑367/+128) ymcC ← / ← serX predicted outer membrane lipoprotein/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported705,8700AG30.8% 14.3 / 6.9 13intergenic (‑367/+128)ymcC/serXpredicted outer membrane lipoprotein/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (3/6);  new base G (2/2);  total (5/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01

CGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCC  >  Exported/705845‑705937
                         |                                                                   
cgctcgcCCTGCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGaa                          <  1:3970083/69‑1 (MQ=14)
     gcCCTGCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCg                     <  1:1236457/69‑1 (MQ=25)
      cccTGCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTc                  >  1:1887858/1‑71 (MQ=21)
       ccTGCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCg                 >  1:4486181/1‑71 (MQ=21)
             ggCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCtt           <  1:3227550/71‑1 (MQ=34)
                aGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtctc        <  1:2095831/71‑1 (MQ=34)
                aGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtctc        <  1:3079791/71‑1 (MQ=34)
                aGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtctc        <  1:450687/71‑1 (MQ=34)
                aGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtct         >  1:1376602/1‑70 (MQ=35)
                  cccGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCAt      <  1:2412503/71‑1 (MQ=34)
                     gCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCtt           <  1:1243759/63‑1 (MQ=35)
                      cTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATcccc  >  1:1884776/1‑71 (MQ=34)
                       tCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtct         >  1:1333346/1‑63 (MQ=35)
                         |                                                                   
CGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCC  >  Exported/705845‑705937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: