Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	422,197	G→A	6.4%	F94F (TTC→TTT)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	422,197	0	G	A	6.4%	306.4 / 8.4	93	F94F (TTC→TTT)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base G (37/50);  new base A (2/4);  total (39/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

AGTCGTCGAGAAATTCCGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTA  >  Exported/422127‑422267                                                                       |                                                                       aGTCGTCGAGAAATTCCGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTgg                                                                        <  1:2089919/71‑1 (MQ=255)   tcgtcgAGAAATTCCGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGaa                                                                      <  1:3157264/71‑1 (MQ=255)   tcgtcgAGAAATTCCGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGaa                                                                      <  1:3801739/71‑1 (MQ=255)   tcgtcgAGAAATTCCGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGaa                                                                      <  1:1148816/71‑1 (MQ=255)   tcgtcgAGAAATTCCGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTCGaa                                                                      <  1:214470/71‑1 (MQ=255)    cgtcgAGAAATTCCGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAAc                                                                     >  1:474052/1‑71 (MQ=255)       cgAGAAATTCCGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTgg                                                                  >  1:2351055/1‑71 (MQ=255)       cgAGAAATTCCGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTgg                                                                  >  1:4280049/1‑71 (MQ=255)       cgAGAAATTCCGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTgg                                                                  >  1:1566470/1‑71 (MQ=255)       cgAGAAATTCCGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTgg                                                                  >  1:827128/1‑71 (MQ=255)        gagaAATTCCGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTgg                                                                  >  1:2489150/1‑70 (MQ=255)           aaaTTCCGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGCCCCGGTCTGGAACTGGTAAc                                                              >  1:1465961/1‑71 (MQ=255)           aaaTTCCGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTaa                                                               >  1:2700703/1‑70 (MQ=255)              ttCCGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGAt                                                           <  1:4111059/71‑1 (MQ=255)                ccGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACACGGTCTGAAACTGGTAACGATc                                                          <  1:911640/70‑1 (MQ=255)                 cGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCg                                                         <  1:4339266/70‑1 (MQ=255)                 cGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCg                                                         <  1:4538966/70‑1 (MQ=255)                    cccTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGcc                                                       >  1:3768843/1‑69 (MQ=255)                    cccTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCa                                                      >  1:3967211/1‑70 (MQ=255)                     ccTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCata                                                    >  1:3749188/1‑71 (MQ=255)                     ccTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCata                                                    <  1:2616480/71‑1 (MQ=255)                     ccTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCata                                                    >  1:3085572/1‑71 (MQ=255)                     ccTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCata                                                    <  1:3187909/71‑1 (MQ=255)                     ccTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCata                                                    <  1:2371360/71‑1 (MQ=255)                     ccTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCata                                                    <  1:2343157/71‑1 (MQ=255)                     ccTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCata                                                    >  1:425361/1‑71 (MQ=255)                     ccTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCata                                                    <  1:4255355/71‑1 (MQ=255)                       tttttCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCatat                                                   >  1:4268623/1‑70 (MQ=255)                        ttttCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTc                                                 >  1:1929563/1‑71 (MQ=255)                         tttCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTc                                                 <  1:4263561/70‑1 (MQ=255)                         tttCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCg                                                >  1:1106922/1‑71 (MQ=255)                         tttCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCg                                                >  1:3754217/1‑71 (MQ=255)                            ccTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGgc                                              <  1:2694941/70‑1 (MQ=255)                              tGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGgcgcg                                           >  1:395097/1‑71 (MQ=255)                                aTACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGgcgcgc                                          <  1:4003925/70‑1 (MQ=255)                                aTACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGgcgcgc                                          <  1:3048592/70‑1 (MQ=255)                                aTACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGgcgcgc                                          <  1:2990262/70‑1 (MQ=255)                                aTACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGgcgcgc                                          >  1:1509187/1‑70 (MQ=255)                                aTACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGgcgcgc                                          <  1:977826/70‑1 (MQ=255)                                aTACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCa                                         <  1:3602881/71‑1 (MQ=255)                                aTACAGCGCGAGGATCACTTCAAAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGgcgcg                                           <  1:1654261/69‑1 (MQ=255)                                 tACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCaa                                        <  1:3387112/71‑1 (MQ=255)                                  aCAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGgcgcgc                                          <  1:1009166/68‑1 (MQ=255)                                    aGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGc                                     >  1:3395602/1‑71 (MQ=255)                                     gcgcGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGgcgcgc                                          <  1:3890099/65‑1 (MQ=255)                                       gcgAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGAt                                                           >  1:1021170/1‑46 (MQ=255)                                       gcgAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGAt                                                           >  1:108722/1‑46 (MQ=255)                                       gcgAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGAt                                                           >  1:1297442/1‑46 (MQ=255)                                       gcgAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGAt                                                           >  1:2410195/1‑46 (MQ=255)                                       gcgAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGAt                                                           >  1:2775224/1‑46 (MQ=255)                                         gAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCtggt                                <  1:314004/71‑1 (MQ=255)                                         gAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCtggt                                <  1:838067/71‑1 (MQ=255)                                         gAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCtggt                                <  1:2069825/71‑1 (MQ=255)                                           ggATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACg                                                             <  1:4369007/40‑1 (MQ=255)                                           ggATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACg                                                             <  1:3317822/40‑1 (MQ=255)                                            gATCACTTCACAGTCAGACCCGGTCTGGAACTGGt                                                                 <  1:829123/35‑1 (MQ=255)                                            gATCACTTCACAGTCAGACCCGGTCTGGAACTGGt                                                                 <  1:531216/35‑1 (MQ=255)                                             aTCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCg                                                >  1:4121429/1‑51 (MQ=255)                                             aTCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGgcgcgc                                          <  1:148164/57‑1 (MQ=255)                                             aTCACTTCACAGTCAGACCCGGTCAGGAACTGGTAACGAt                                                           >  1:2970645/1‑40 (MQ=255)                                               cACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTg                           >  1:3412202/1‑70 (MQ=255)                                               cACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTg                           >  1:390297/1‑70 (MQ=255)                                               cACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTg                           >  1:3910070/1‑70 (MQ=255)                                                aCTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTa                         <  1:2179021/71‑1 (MQ=255)                                                 cTTCACAGTCAGACCCGGTCTGAAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTg                           >  1:1734320/1‑68 (MQ=255)                                                 cTTCACAGTCAGACCCGGTCTGAAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTg                           >  1:4731649/1‑68 (MQ=255)                                                 cTTCACAGTCAGACCCGGTCTGAAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAg                        <  1:3384836/71‑1 (MQ=255)                                                 cTTCACAGTCAGACCCGGTCTGAAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAg                        <  1:4021497/71‑1 (MQ=255)                                                 cTTCACAGTCAGACCCGGTCTGAAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAg                        <  1:4435157/71‑1 (MQ=255)                                                  ttCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCtgg                                 <  1:3149626/61‑1 (MQ=255)                                                  ttCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCtgg                                 <  1:4739221/61‑1 (MQ=255)                                                  ttCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCtgg                                 <  1:3808430/61‑1 (MQ=255)                                                   tCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCat                                                     >  1:3064736/1‑40 (MQ=255)                                                    cacaGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCg                                                >  1:803251/1‑44 (MQ=255)                                                    cacaGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGAt                      <  1:2997070/70‑1 (MQ=255)                                                    cacaGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGAt                      <  1:871338/70‑1 (MQ=255)                                                     acaGTCAGACCCGGTCTGGAACTGGTAACGATCTCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGAttt                    <  1:4054507/71‑1 (MQ=255)                                                     acaGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGAttt                    <  1:2379854/71‑1 (MQ=255)                                                       aGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCa                  >  1:2340943/1‑71 (MQ=255)                                                       aGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCa                  >  1:907325/1‑71 (MQ=255)                                                           aGACCCGGTCTGGAACTGGTAACGATCGCCATAtt                                                  >  1:176759/1‑35 (MQ=255)                                                           aGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCTCGCAATGCCTGGTGGTTGTAGATTTCACCGt              <  1:2350164/71‑1 (MQ=255)                                                           aGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCg               >  1:821615/1‑70 (MQ=255)                                                           aGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGt              >  1:1123239/1‑71 (MQ=255)                                                             aCCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGttt            <  1:3623829/71‑1 (MQ=255)                                                             aCCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGttt            <  1:858478/71‑1 (MQ=255)                                                                 ggTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCtgg                                 <  1:4503345/46‑1 (MQ=255)                                                                 ggTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCtgg                                 <  1:832055/46‑1 (MQ=255)                                                                  gTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGc       <  1:212807/71‑1 (MQ=255)                                                                  gTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGc       <  1:2990815/71‑1 (MQ=255)                                                                  gTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGc       <  1:386832/71‑1 (MQ=255)                                                                   tCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCt                                   <  1:377734/42‑1 (MQ=255)                                                                   tCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGcc      <  1:2972209/71‑1 (MQ=255)                                                                   tCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGcc      <  1:111607/71‑1 (MQ=255)                                                                      ggAACTGGTAACGATCGCCATATTCGGCGCGCATTGCCt                                   <  1:3216108/39‑1 (MQ=255)                                                                       gAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAg    <  1:2554279/69‑1 (MQ=255)                                                                       gAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTa  >  1:2613618/1‑71 (MQ=255)                                                                       gAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTa  >  1:872205/1‑71 (MQ=255)                                                                       gAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTa  >  1:1269832/1‑71 (MQ=255)                                                                       |                                                                       AGTCGTCGAGAAATTCCGGCCCTTTTTCCTGATACAGCGCGAGGATCACTTCACAGTCAGACCCGGTCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTA  >  Exported/422127‑422267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑