Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 415,949 C→G 5.0% S257T (AGC→ACC) ‡ ybeZ ← hypothetical protein with nucleoside triphosphate hydrolase domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported415,9490CG 5.0% 334.3 / 5.0 99S257T (AGC→ACC) ‡ybeZhypothetical protein with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base C (53/41);  new base G (3/2);  total (56/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 111 total aligned reads displayed.

GATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAA  >  Exported/415879‑416018
                                                                      |                                                                     
gATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGc                                                                       >  1:760082/1‑71 (MQ=255)
gATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGc                                                                       >  1:3058134/1‑71 (MQ=255)
  tAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctc                                                                     >  1:3090465/1‑71 (MQ=255)
  tAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctc                                                                     >  1:2372690/1‑71 (MQ=255)
    aCCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctct                                                                    >  1:2930438/1‑70 (MQ=255)
    aCCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctct                                                                    >  1:2842279/1‑70 (MQ=255)
    aCCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctct                                                                    <  1:3918815/70‑1 (MQ=255)
    aCCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctct                                                                    <  1:1882918/70‑1 (MQ=255)
      cGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCtctc                                                                   >  1:1323739/1‑69 (MQ=255)
      cGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCAt                                                                 >  1:1093753/1‑71 (MQ=255)
      cGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCAt                                                                 >  1:78793/1‑71 (MQ=255)
      cGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCAt                                                                 >  1:731179/1‑71 (MQ=255)
      cGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTACATCTGTTCGATGGTGGTGTTCTGGCTCTCAt                                                                 >  1:3778705/1‑71 (MQ=255)
       gCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATc                                                                >  1:1367924/1‑71 (MQ=255)
        cTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTTTTCGATGGTGGTGTTCTGGCTCTCATCg                                                               <  1:3951505/71‑1 (MQ=255)
        cTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATc                                                                >  1:3432625/1‑70 (MQ=255)
        cTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCGGATCg                                                               >  1:2705049/1‑71 (MQ=255)
         ttttGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATc                                                                >  1:3404131/1‑69 (MQ=255)
           ttGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCgag                                                             >  1:1800460/1‑70 (MQ=255)
           ttGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCgag                                                             >  1:280283/1‑70 (MQ=255)
           ttGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCgag                                                             >  1:4240540/1‑70 (MQ=255)
            tGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa                                                           >  1:1530854/1‑71 (MQ=255)
               gTTAAAACCGATACGGGTCATGAACATCTTCATCTGTTCGATGTTGGTGTTCTGGCTCTCAt                                                                 >  1:3607507/1‑62 (MQ=255)
                 tAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGAt                                                       >  1:4357761/1‑70 (MQ=255)
                  aaaaCCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATa                                                      >  1:2623437/1‑70 (MQ=255)
                      ccGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAAcgc                                                 >  1:2976042/1‑71 (MQ=255)
                       cGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAAcgcg                                                <  1:2875490/71‑1 (MQ=255)
                        gATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGcgt                                               <  1:2846761/71‑1 (MQ=255)
                            cGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGcgtcgt                                            <  1:4644514/70‑1 (MQ=255)
                              ggTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCa                                         <  1:179884/71‑1 (MQ=255)
                                  agtaaCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGt                                     <  1:2819391/68‑1 (MQ=255)
                                  aGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTGAGCGt                                     <  1:534097/71‑1 (MQ=255)
                                  aGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGt                                     <  1:3031544/71‑1 (MQ=255)
                                  aGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGt                                     <  1:3891284/71‑1 (MQ=255)
                                  aGGAACATCTTCATCTGCTCGACGGTCGTGTTCTGGGCTTCGTCCAGGATCACGAACGCGTGGTTc                                          >  1:3525706/1‑66 (MQ=255)
                                  aGGAACATCTTCATCTGCTCGACGGTCGTGTTCTGGGCTTCGTCCAGGATCACGAACGCGTGGTTCAgg                                       >  1:1431861/1‑68 (MQ=255)
                                   ggAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGt                                     <  1:370660/70‑1 (MQ=255)
                                    gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTa                                    >  1:4744366/1‑70 (MQ=255)
                                    gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTa                                    >  1:3005192/1‑70 (MQ=255)
                                    gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc                                   >  1:3465459/1‑71 (MQ=255)
                                    gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc                                   <  1:1523595/71‑1 (MQ=255)
                                    gAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc                                   >  1:2057428/1‑71 (MQ=255)
                                     aaCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc                                   >  1:4577611/1‑70 (MQ=255)
                                      aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa                                                           >  1:2556841/1‑45 (MQ=255)
                                      aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa                                                           >  1:2735683/1‑45 (MQ=255)
                                      aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa                                                           >  1:2467472/1‑45 (MQ=255)
                                      aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGaa                                                           >  1:2204159/1‑45 (MQ=255)
                                      aCATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACg                                  <  1:1101927/70‑1 (MQ=255)
                                       cATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATa                                                      <  1:4115012/49‑1 (MQ=255)
                                       cATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATa                                                      <  1:4088829/49‑1 (MQ=255)
                                        aTCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCTTCGAGAATGATa                                                      <  1:3324702/48‑1 (MQ=255)
                                           ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCTTCGAGAATGATAAACGCGTCGTTCAGCGTAc                                   >  1:1895402/1‑64 (MQ=255)
                                           ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATaa                                                     >  1:3380947/1‑46 (MQ=255)
                                           ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATaa                                                     >  1:2655258/1‑46 (MQ=255)
                                           ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTa                                    >  1:2112583/1‑63 (MQ=255)
                                           ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc                                   >  1:2840705/1‑64 (MQ=255)
                                           ttCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTAc                                   >  1:3156393/1‑64 (MQ=255)
                                             cATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCAc                             <  1:531802/68‑1 (MQ=255)
                                              aTCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCa                          <  1:3825442/70‑1 (MQ=255)
                                               tCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                        >  1:1646717/1‑71 (MQ=255)
                                               tCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                        >  1:3198458/1‑71 (MQ=255)
                                                 tGTTCGATGGTGGTGTTCTGGCTCTCATCGAGGATGATAAACGCGTCGTTCAGCGTACGACCACGCata                        >  1:1186726/1‑69 (MQ=255)
                                                  gTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                        >  1:302678/1‑68 (MQ=255)
                                                   ttCGATGGTTGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                        <  1:165374/67‑1 (MQ=255)
                                                   ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACg                                  <  1:3809886/57‑1 (MQ=255)
                                                   ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                        <  1:4196811/67‑1 (MQ=255)
                                                   ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                        <  1:2711926/67‑1 (MQ=255)
                                                   ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                        <  1:2727805/67‑1 (MQ=255)
                                                   ttCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                        <  1:4695784/67‑1 (MQ=255)
                                                   atCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAACCGCGTCGTTCAGCGTACGACCACGCata                        <  1:3172875/66‑1 (MQ=255)
                                                     cGATGGTGGTGTTCTGGCTCTCATCGAGAATGATaaa                                                    >  1:2341350/1‑37 (MQ=255)
                                                     cGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGCCCACGCATATAGGc                   >  1:588577/1‑70 (MQ=255)
                                                     cGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGc                   >  1:1185104/1‑70 (MQ=255)
                                                     cGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGc                   >  1:3962830/1‑70 (MQ=255)
                                                      gATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGcc                  <  1:2045746/70‑1 (MQ=255)
                                                      gATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGcc                  <  1:1113756/70‑1 (MQ=255)
                                                       aTGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGcc                  <  1:4052627/69‑1 (MQ=255)
                                                        tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc               <  1:4197899/71‑1 (MQ=255)
                                                        tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc               <  1:4750944/71‑1 (MQ=255)
                                                        tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc               <  1:1696876/71‑1 (MQ=255)
                                                        tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc               >  1:1865383/1‑71 (MQ=255)
                                                        tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc               <  1:2613602/71‑1 (MQ=255)
                                                        tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc               <  1:3428267/71‑1 (MQ=255)
                                                        tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc               <  1:2253320/71‑1 (MQ=255)
                                                        tggtggTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGc               <  1:2255534/71‑1 (MQ=255)
                                                        cggtcgtGTTCTGGGCTTCGTCCAGGATCACGAACGCGTGGTTCAGGGTGCGCCCGCGCATATAGGCCAGc               <  1:474649/70‑1 (MQ=255)
                                                        cggtcgtGTTCTGGGCTTCGTCCAGGATCACGAACGCGTGGTTCAGGGTGCGCCCGCGCATATAGGCCAGc               <  1:654269/70‑1 (MQ=255)
                                                          gtggtgTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCg              <  1:2001596/70‑1 (MQ=255)
                                                           tggtgTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGt            <  1:840300/71‑1 (MQ=255)
                                                           tggtgTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGt            <  1:2768528/71‑1 (MQ=255)
                                                            ggtgTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGt            >  1:3322729/1‑70 (MQ=255)
                                                            ggtgTTCTGGCTCTAATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGt            >  1:557101/1‑70 (MQ=255)
                                                             gtgtTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGc          >  1:849650/1‑71 (MQ=255)
                                                             gtgtGCTGGCTCTCATCGAGAATGATAAACGCGGCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGc          <  1:3044325/71‑1 (MQ=255)
                                                                  cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCa                                         >  1:3556052/1‑35 (MQ=255)
                                                                  cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCa                                         >  1:3487815/1‑35 (MQ=255)
                                                                  cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                        >  1:4221569/1‑52 (MQ=255)
                                                                  cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                        >  1:206683/1‑52 (MQ=255)
                                                                  cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                        >  1:842776/1‑52 (MQ=255)
                                                                  cTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACtt     >  1:760054/1‑71 (MQ=255)
                                                                      |                                                                     
GATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAA  >  Exported/415879‑416018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: