| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 17,199 | G→T | 12.5% | intergenic (+3/‑57) | nhaA → / → nhaR | sodium‑proton antiporter/DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 17,199 | 0 | G | T | 12.5% | 285.3 / 16.3 | 88 | intergenic (+3/‑57) | nhaA/nhaR | sodium‑proton antiporter/DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (46/31); new base T (3/8); total (49/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
CGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCA > Exported/17130‑17269 | cGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAg > 1:2589897/1‑70 (MQ=255) gTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGAc < 1:1273845/71‑1 (MQ=255) cTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGt > 1:516540/1‑71 (MQ=255) cTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGt > 1:4044755/1‑71 (MQ=255) tctcTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGttt < 1:3332194/70‑1 (MQ=255) tctcTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGttt < 1:3850004/70‑1 (MQ=255) tctcTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGttt < 1:4634765/70‑1 (MQ=255) tctcTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGttt < 1:2242911/70‑1 (MQ=255) tctTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGtt > 1:1442564/1‑67 (MQ=255) tctTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGtt > 1:4000789/1‑67 (MQ=255) ttCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGTGTCCATCAGTTTGACATGACGGTTTAcc < 1:3031382/69‑1 (MQ=255) ttCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACATGACGGTTTAcc < 1:1331270/69‑1 (MQ=255) ttCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACATGACGGTTTAcc < 1:407915/69‑1 (MQ=255) ttCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACATGACGGTTTAcc < 1:1817076/69‑1 (MQ=255) ttCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACATGACGGTTTACCgg > 1:2054125/1‑71 (MQ=255) ttCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGt > 1:2836791/1‑64 (MQ=255) ttCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTAcc < 1:3748994/69‑1 (MQ=255) ttCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCgg > 1:1802588/1‑71 (MQ=255) ttCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCgg > 1:1494438/1‑71 (MQ=255) ttCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCgg > 1:3359817/1‑71 (MQ=255) ttCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACAgg > 1:4359899/1‑71 (MQ=255) ttCGGCGGTAATTGGATACAGCTGGATACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCgg > 1:450024/1‑71 (MQ=255) cggcggTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCggg < 1:3197028/70‑1 (MQ=255) ggTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGc > 1:1752296/1‑70 (MQ=255) gTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGAc < 1:3604364/54‑1 (MQ=255) aaTTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGtt > 1:3921720/1‑56 (MQ=255) aaTTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGtt > 1:2225/1‑56 (MQ=255) aaTTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGtt > 1:2695853/1‑56 (MQ=255) aaTTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGtt > 1:2630002/1‑56 (MQ=255) aaTTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGtt > 1:2289888/1‑56 (MQ=255) aaTTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCAt > 1:3908387/1‑70 (MQ=255) aTTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATaa < 1:1184777/71‑1 (MQ=255) tGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGAc > 1:1857916/1‑49 (MQ=255) ggATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCTATAAAc < 1:607905/70‑1 (MQ=255) ggATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAAc < 1:2876407/70‑1 (MQ=255) aTACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGCTTTACCGGGGAGCCATAAACGGc > 1:638899/1‑71 (MQ=255) tACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCt < 1:3192512/71‑1 (MQ=255) aCAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGt > 1:2765572/1‑47 (MQ=255) aCAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGt > 1:3282804/1‑47 (MQ=255) cAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTa > 1:645983/1‑49 (MQ=255) cAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTa > 1:788911/1‑49 (MQ=255) cAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTcc > 1:4001054/1‑71 (MQ=255) gTTACGCGTTCGTTTGCGTCCATCAGTTTGACATGACGGTTTACCGGGGAGCCATAAACGGCTCCCtttt < 1:2424992/70‑1 (MQ=255) gTTACGCGTTCGTTTGCGTCCATCAGTTTGACATGACGGTTTACCGGGGAGCCATAAACGGCTCCCtttt < 1:2770790/70‑1 (MQ=255) ttACGCGTTCTTTTGCGTCCATCATTTTGACATGACGGTTTACCGGGGAGCCATAAACGGCTCCCtttt < 1:4035379/69‑1 (MQ=255) ttACGCGTTCGTTTGCGTCCATCAGTTTGACATGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCa < 1:1995302/71‑1 (MQ=255) cgcgTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCg < 1:669133/42‑1 (MQ=255) cgcgTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCAtt < 1:174868/70‑1 (MQ=255) cgcgTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCAtt < 1:3801307/70‑1 (MQ=255) gcgTTCGTTTGCGTCCATCAGTTTGACATGACGGTTTACCggg > 1:3257988/1‑43 (MQ=255) gcgTTCGTTTGCGTCCATCAGTTTGACATGACGGTTTACCGGGGAGCCATAAACGGCTCCCt > 1:3993660/1‑62 (MQ=255) gttcgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCt > 1:1664891/1‑60 (MQ=255) gttcgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCAt > 1:2386564/1‑66 (MQ=255) ttcgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATaa < 1:1439009/49‑1 (MQ=255) ttcgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATa < 1:2285760/48‑1 (MQ=255) ttcgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATa < 1:1819600/48‑1 (MQ=255) tcgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATa < 1:303682/47‑1 (MQ=255) tcgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTAt > 1:3567437/1‑70 (MQ=255) tcgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTAt > 1:3524898/1‑70 (MQ=255) cgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATa < 1:221280/46‑1 (MQ=255) cgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATa < 1:214683/46‑1 (MQ=255) cgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTa > 1:1705895/1‑68 (MQ=255) cgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTAt > 1:2576548/1‑69 (MQ=255) ttGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGt > 1:912056/1‑63 (MQ=255) ttGCCTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTg > 1:3627296/1‑62 (MQ=255) tGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAgg > 1:4712141/1‑69 (MQ=255) cGTCCATCAGTTTGACAGGACGGTTTACCGGGGAg < 1:4583118/35‑1 (MQ=255) tCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGgagaga < 1:633321/71‑1 (MQ=255) tCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGgagaga < 1:2278898/71‑1 (MQ=255) tCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGgagaga < 1:363004/71‑1 (MQ=255) cATCAGTTTGACAGGACGGTTTACCGGGTAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGgagaga < 1:2737865/69‑1 (MQ=255) cATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGgagaga < 1:799131/69‑1 (MQ=255) aTCAGTTTGACAGGACGGTTTACCGGGGAGCCATaaa > 1:841822/1‑37 (MQ=255) cAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTAt < 1:590116/56‑1 (MQ=255) tGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATg > 1:952427/1‑65 (MQ=255) tGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCAt < 1:3168425/70‑1 (MQ=255) tGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATg > 1:2498559/1‑71 (MQ=255) tGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATg > 1:1314814/1‑71 (MQ=255) tGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATg > 1:1979086/1‑71 (MQ=255) tGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATg > 1:2219552/1‑71 (MQ=255) gACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATg < 1:116884/70‑1 (MQ=255) aCAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTg > 1:1476922/1‑45 (MQ=255) aCAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGaaa > 1:3030030/1‑61 (MQ=255) aCAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGtc > 1:781610/1‑71 (MQ=255) cAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTTATTGTTATCAGGGAGAGAAATGAGCATGtc > 1:3390427/1‑70 (MQ=255) cAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGtct < 1:2213869/71‑1 (MQ=255) cAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGtc > 1:4120109/1‑70 (MQ=255) cAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGtc > 1:3794437/1‑70 (MQ=255) cAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGtc > 1:2022096/1‑70 (MQ=255) ggACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTc < 1:2763352/38‑1 (MQ=255) ggACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCa < 1:1781743/71‑1 (MQ=255) | CGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCA > Exported/17130‑17269 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |