| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,973,690 | A→T | 11.3% | K29I (AAA→ATA) | nadR → | bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,973,690 | 0 | A | T | 11.3% | 176.0 / 12.5 | 62 | K29I (AAA→ATA) | nadR | bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (25/30); new base T (5/2); total (30/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTG > Exported/2973625‑2973757 | gAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCaaa > 1:841307/1‑67 (MQ=255)gAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCaaa > 1:3095208/1‑67 (MQ=255)gAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCaaa > 1:4000012/1‑67 (MQ=255) tCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGc < 1:2582822/68‑1 (MQ=255) tCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGc < 1:2279342/68‑1 (MQ=255) tCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGc < 1:4048156/68‑1 (MQ=255) tCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAg > 1:3404156/1‑71 (MQ=255) tCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAg > 1:111352/1‑71 (MQ=255) cAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt > 1:4372720/1‑71 (MQ=255) aaGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAg > 1:3354259/1‑69 (MQ=255) aaGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAg > 1:1843355/1‑69 (MQ=255) aaGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAg > 1:4593447/1‑69 (MQ=255) aaGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAg > 1:2880836/1‑69 (MQ=255) aaGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt > 1:3457352/1‑70 (MQ=255) aaGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt < 1:983123/70‑1 (MQ=255) aaGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt > 1:3201927/1‑70 (MQ=255) aaGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt > 1:2648287/1‑70 (MQ=255) aaGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt > 1:4618485/1‑70 (MQ=255) gCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTAc > 1:1350745/1‑71 (MQ=255) gCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTAc > 1:3964012/1‑71 (MQ=255) aaCAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTg < 1:2603542/71‑1 (MQ=255) aaCAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTg < 1:2687234/71‑1 (MQ=255) aaCAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACt > 1:3754240/1‑70 (MQ=255) gggCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACt < 1:2150659/66‑1 (MQ=255) tGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt < 1:1205862/58‑1 (MQ=255) tGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt < 1:3289576/58‑1 (MQ=255) tGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt < 1:2566672/58‑1 (MQ=255) gCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGt < 1:1273889/45‑1 (MQ=255) cGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATc < 1:1817518/71‑1 (MQ=255) cGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATc < 1:3437686/71‑1 (MQ=255) gCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGGTACTGAATGCCAAAATCa < 1:270701/71‑1 (MQ=255) tACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaa > 1:4436495/1‑70 (MQ=255) cagGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGccc < 1:4001756/71‑1 (MQ=255) cagGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGccc < 1:2504196/71‑1 (MQ=255) cagGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGccc < 1:799019/71‑1 (MQ=255) cagGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGccc > 1:1963009/1‑71 (MQ=255) tAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCa > 1:842106/1‑40 (MQ=255) tAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCa > 1:3361736/1‑40 (MQ=255) aGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAgcg > 1:1248488/1‑71 (MQ=255) cTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTg < 1:4142309/44‑1 (MQ=255) cTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAgcgcg > 1:4696916/1‑71 (MQ=255) gATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGaa > 1:3341103/1‑44 (MQ=255) gATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGaa > 1:4162558/1‑44 (MQ=255) gATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAgcgcgc > 1:2249900/1‑70 (MQ=255) tGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCaa > 1:16469/1‑70 (MQ=255) gCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCaaa < 1:3937743/48‑1 (MQ=255) gCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCaaa < 1:2543088/48‑1 (MQ=255) ccAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAg < 1:3882356/71‑1 (MQ=255) cAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGc < 1:3991995/71‑1 (MQ=255) cAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGc < 1:2463657/71‑1 (MQ=255) gCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTg > 1:971031/1‑71 (MQ=255) gCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTg < 1:4001743/71‑1 (MQ=255) gCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTg < 1:471845/71‑1 (MQ=255) cGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGcc < 1:3229165/53‑1 (MQ=255) cGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTgg < 1:1647885/71‑1 (MQ=255) ggTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGa < 1:893179/71‑1 (MQ=255) aTGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGc > 1:1662307/1‑71 (MQ=255) tGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCaaaa < 1:1925584/39‑1 (MQ=255) tGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCaaaa < 1:3800879/39‑1 (MQ=255) tGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCg < 1:4255762/71‑1 (MQ=255) tGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCg > 1:1464227/1‑71 (MQ=255) tGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCg > 1:892185/1‑71 (MQ=255) aCCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGt < 1:122715/70‑1 (MQ=255) ccATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTg > 1:833165/1‑62 (MQ=255) cAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGGGCGCAAAAGCTGGAGGCGTTg > 1:4198935/1‑70 (MQ=255) | GAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTG > Exported/2973625‑2973757 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |