Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,948,260 G→A 14.3% intergenic (‑20/+118) yjjB ← / ← yjjP conserved inner membrane protein/predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,948,2600GA14.3% 400.2 / 48.3 147intergenic (‑20/+118)yjjB/yjjPconserved inner membrane protein/predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (67/59);  new base A (14/7);  total (81/66)
Fisher's exact test for biased strand distribution p-value = 3.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

ATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGG  >  Exported/2948194‑2948330
                                                                  |                                                                      
aTAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGc                                                                     >  1:2170750/1‑70 (MQ=255)
aTAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGc                                                                     >  1:1440043/1‑70 (MQ=255)
aTAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGc                                                                     >  1:2844114/1‑70 (MQ=255)
aTAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGc                                                                     >  1:1191324/1‑70 (MQ=255)
   aaGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCAAGCCAag                                                                 >  1:2785182/1‑71 (MQ=255)
    aGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAagc                                                                <  1:4103240/71‑1 (MQ=255)
    aGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAag                                                                 >  1:69086/1‑70 (MQ=255)
    aGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAag                                                                 >  1:113064/1‑70 (MQ=255)
    aGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAag                                                                 >  1:1709755/1‑70 (MQ=255)
       ttGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAagcagc                                                             <  1:2360825/71‑1 (MQ=255)
       ttGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAagcagc                                                             <  1:2441330/71‑1 (MQ=255)
             aCTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCacac                                                       <  1:3390058/71‑1 (MQ=255)
             aCTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCAAGCCAAGCAGCGcaca                                                        >  1:1526874/1‑70 (MQ=255)
             aCTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCAAGCCAAGCAGCGCacac                                                       >  1:1810760/1‑71 (MQ=255)
              cTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCacac                                                       >  1:2929724/1‑70 (MQ=255)
                   tatTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAagca                                                               <  1:2879346/57‑1 (MQ=255)
                   tatTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCg                                                  >  1:4744657/1‑70 (MQ=255)
                     ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGca                                                          >  1:3471072/1‑60 (MQ=255)
                     ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGca                                                          >  1:2552483/1‑60 (MQ=255)
                     ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACAcc                                                      >  1:1320433/1‑64 (MQ=255)
                     ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACAcc                                                      >  1:2188746/1‑64 (MQ=255)
                     ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa                                               >  1:3929775/1‑71 (MQ=255)
                     ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa                                               <  1:93953/71‑1 (MQ=255)
                     ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa                                               <  1:1817201/71‑1 (MQ=255)
                     ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTa                                                <  1:4622698/70‑1 (MQ=255)
                     ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTa                                                <  1:1002263/70‑1 (MQ=255)
                     ttCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTa                                                <  1:3268624/70‑1 (MQ=255)
                      tCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTa                                                <  1:3320450/69‑1 (MQ=255)
                      tCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCAAGCCAAGCAGCGCACACCAGCGTAAc                                              >  1:2602424/1‑71 (MQ=255)
                       cAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAAc                                              <  1:4495578/70‑1 (MQ=255)
                        aaCCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGc                                            <  1:1122815/71‑1 (MQ=255)
                             gCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCa                                       >  1:3259100/1‑71 (MQ=255)
                              cTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCa                                       >  1:2997860/1‑70 (MQ=255)
                              cTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCAc                                      >  1:1517398/1‑71 (MQ=255)
                               tGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACg                                     >  1:2622193/1‑71 (MQ=255)
                                  tcatcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGt                                                 <  1:892580/56‑1 (MQ=255)
                                  tcatcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACggg                                   >  1:1425906/1‑70 (MQ=255)
                                    atcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGt                                                 <  1:1240214/54‑1 (MQ=255)
                                    atcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGt                                                 <  1:370610/54‑1 (MQ=255)
                                    atcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGa                                  <  1:2056532/69‑1 (MQ=255)
                                    atcaAGATCATTCGGGAACCATGACCTATCAAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAAc                                <  1:2691673/71‑1 (MQ=255)
                                     tcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCg                                                  <  1:4659104/52‑1 (MQ=255)
                                     tcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGt                                                 <  1:4007320/53‑1 (MQ=255)
                                     tcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGt                                                 <  1:790903/53‑1 (MQ=255)
                                     tcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACg                               <  1:2114302/71‑1 (MQ=255)
                                     tcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACg                               <  1:3593946/71‑1 (MQ=255)
                                     tcaAGATCATTCGGGAACCATGACCTATCAAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGaa                                 <  1:446410/69‑1 (MQ=255)
                                      caAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACgg                                    >  1:2800651/1‑65 (MQ=255)
                                       aaGATCATTCGGGAACCATGACCTATCGAGCCAagcag                                                              >  1:550169/1‑38 (MQ=255)
                                       aaGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa                                               >  1:3525184/1‑53 (MQ=255)
                                       aaGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa                                               >  1:2578425/1‑53 (MQ=255)
                                       aaGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa                                               >  1:2510304/1‑53 (MQ=255)
                                       aaGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa                                               >  1:2926087/1‑53 (MQ=255)
                                       aaGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTaa                                               >  1:2998567/1‑53 (MQ=255)
                                       aaGATCATTCGGGAACCATGACCTATCAAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGtt                             >  1:3458161/1‑71 (MQ=255)
                                         gATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGcac                                                         <  1:4634102/41‑1 (MQ=255)
                                         gATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGa                           <  1:2328527/71‑1 (MQ=255)
                                         gATCATTCGGGAACCATGACCTATCAAGCCAagcagc                                                             >  1:3114636/1‑37 (MQ=255)
                                         gATCATTCGGGAACCATGACCTATCAAGCCAagcagc                                                             >  1:3719657/1‑37 (MQ=255)
                                          aTCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACg                                             >  1:4187549/1‑52 (MQ=255)
                                          aTCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACg                                             >  1:2059551/1‑52 (MQ=255)
                                           tCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCg                                                  <  1:509010/46‑1 (MQ=255)
                                           tCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCg                                                  <  1:4625000/46‑1 (MQ=255)
                                           tCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCg                                                  <  1:2988116/46‑1 (MQ=255)
                                           tCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCg                                                  <  1:1873206/46‑1 (MQ=255)
                                           tCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCg                                                  <  1:1882029/46‑1 (MQ=255)
                                            cATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGcc                                           >  1:1698297/1‑52 (MQ=255)
                                            cATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGcc                                           >  1:3168004/1‑52 (MQ=255)
                                            cATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGcc                                           >  1:3146640/1‑52 (MQ=255)
                                            cATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAaca                        >  1:3628746/1‑71 (MQ=255)
                                            cATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAaca                        >  1:1996394/1‑71 (MQ=255)
                                              ttCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACAcc                      <  1:1079692/71‑1 (MQ=255)
                                                cGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAAc                                >  1:2231953/1‑59 (MQ=255)
                                                cGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACg                               >  1:4679410/1‑60 (MQ=255)
                                                cGGGAACCATGACCTATCAAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGtt                             >  1:3522160/1‑62 (MQ=255)
                                                cGGGAACCATGACCTATCAAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGtt                             >  1:2032879/1‑62 (MQ=255)
                                                cGGGAACCATGACCTATCAAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGtt                             >  1:3918303/1‑62 (MQ=255)
                                                 gggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAacac                       <  1:3250544/67‑1 (MQ=255)
                                                 gggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAacac                       <  1:470724/67‑1 (MQ=255)
                                                 gggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAacac                       <  1:2139463/67‑1 (MQ=255)
                                                 gggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAacac                       <  1:3968832/67‑1 (MQ=255)
                                                  ggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACAcc                      <  1:2814496/67‑1 (MQ=255)
                                                  ggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACAcc                      <  1:1184766/67‑1 (MQ=255)
                                                  ggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACAcc                      <  1:125735/67‑1 (MQ=255)
                                                  ggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACAcc                      <  1:546739/67‑1 (MQ=255)
                                                  ggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACAcc                      <  1:2915901/67‑1 (MQ=255)
                                                  ggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACAcc                      <  1:2856463/67‑1 (MQ=255)
                                                   gAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCa                     >  1:1330792/1‑67 (MQ=255)
                                                    aaCCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCa                <  1:3296464/71‑1 (MQ=255)
                                                      ccATGACCTATCAAGCCAAGCAGCGCACACCAGCGTAACTCCCGCACGGGAACGTTGAACACCATCGCaaa              <  1:3987802/71‑1 (MQ=255)
                                                         tGACCTATCGAGCCAAGCCGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACTCCATCg                  >  1:4180392/1‑64 (MQ=255)
                                                         tGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGcc           >  1:2636720/1‑71 (MQ=255)
                                                               aTCGAGCCAAGCAGCGCACACCAGCGTAACGCCCg                                         >  1:1032286/1‑35 (MQ=255)
                                                               aTCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCg      >  1:309413/1‑70 (MQ=255)
                                                                tCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACgg                                    >  1:4368844/1‑39 (MQ=255)
                                                                tCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACgg                                    >  1:4038483/1‑39 (MQ=255)
                                                                tCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACgg                                    >  1:852983/1‑39 (MQ=255)
                                                                tCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGt                              >  1:2118137/1‑45 (MQ=255)
                                                                tCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGt                              >  1:858645/1‑45 (MQ=255)
                                                                  gAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAgg  <  1:4229510/71‑1 (MQ=255)
                                                                  |                                                                      
ATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGG  >  Exported/2948194‑2948330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: