Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,816,539 T→A 7.6% I66N (ATT→AAT)  soxR → DNA‑binding transcriptional dual regulator, Fe‑S center for redox‑sensing

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,816,5390TA 7.6% 338.7 / 14.9 116I66N (ATT→AAT) soxRDNA‑binding transcriptional dual regulator, Fe‑S center for redox‑sensing
Reads supporting (aligned to +/- strand):  ref base T (43/64);  new base A (7/2);  total (50/66)
Fisher's exact test for biased strand distribution p-value = 3.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

CGGCAATCAGCGGCGATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAGGGCATACGTTAAGTGCGAAA  >  Exported/2816471‑2816609
                                                                    |                                                                      
cGGCAATCAGCGGCGATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTg                                                                      <  1:1745542/71‑1 (MQ=255)
 ggCAATCAGCGGCGATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTgg                                                                     <  1:2250602/71‑1 (MQ=255)
 ggCAATCAGCGGCGATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTgg                                                                     <  1:606576/71‑1 (MQ=255)
 ggCAATCAGCGGCGATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTg                                                                      <  1:3501255/70‑1 (MQ=255)
    aaTCAGCGGCGATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCAt                                                                  <  1:3247449/71‑1 (MQ=255)
     aTCAGCGGCGATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCAtt                                                                 <  1:3404956/71‑1 (MQ=255)
     aTCAGCGGCGATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCAtt                                                                 <  1:2294321/71‑1 (MQ=255)
      tCAGCGGCGATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTc                                                                >  1:4184713/1‑71 (MQ=255)
         gcggcgATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGc                                                             <  1:892457/71‑1 (MQ=255)
         gcggcgATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGc                                                             <  1:3182895/71‑1 (MQ=255)
          cggcgATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCt                                                            <  1:3962990/71‑1 (MQ=255)
          cggcgATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCt                                                            <  1:1739344/71‑1 (MQ=255)
            gcgATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTgg                                                          >  1:3038447/1‑71 (MQ=255)
            gcgATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTgg                                                          >  1:993060/1‑71 (MQ=255)
             cgATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGGAATGCCAATACGCt                                                            <  1:2426283/68‑1 (MQ=37)
              gATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCg                                                        <  1:3154233/71‑1 (MQ=255)
               atatAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGGAATGCCAATACGCTga                                                          >  1:1699343/1‑67 (MQ=37)
               atatAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGGAATGCCAATACGCTga                                                          >  1:1348754/1‑67 (MQ=37)
               atatAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGGAATGCCAATACGCTga                                                          >  1:3644334/1‑67 (MQ=37)
               atatAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGGAATGCCAATACGCTga                                                          >  1:2180643/1‑67 (MQ=37)
               atatAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGGAATGCCAATACGCTga                                                          >  1:1452552/1‑67 (MQ=37)
                tataAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGAc                                                      <  1:1445250/71‑1 (MQ=255)
                 ataAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGAc                                                      >  1:2902641/1‑70 (MQ=255)
                  taAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGAc                                                      <  1:2869539/69‑1 (MQ=255)
                  taAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGAc                                                      <  1:3927895/69‑1 (MQ=255)
                  taAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGAc                                                      <  1:188270/69‑1 (MQ=255)
                      cGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTgg                                                >  1:221581/1‑71 (MQ=255)
                        tGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGAc                                                      <  1:3361642/63‑1 (MQ=255)
                        tGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTg                                              <  1:4675041/71‑1 (MQ=255)
                        tGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTg                                              <  1:4437178/71‑1 (MQ=255)
                          aTGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCa                                                    >  1:462443/1‑63 (MQ=255)
                          aTGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCa                                                    >  1:4597438/1‑63 (MQ=255)
                          aTGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCa                                                    >  1:3543787/1‑63 (MQ=255)
                          aTGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCa                                                    >  1:3431427/1‑63 (MQ=255)
                             tgtTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTgg                                                >  1:2597219/1‑64 (MQ=255)
                             tgtTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGc                                          <  1:1096635/70‑1 (MQ=255)
                             tgtTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGc                                          <  1:3903629/70‑1 (MQ=255)
                               ttGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGt                                        <  1:2938746/70‑1 (MQ=255)
                                 gCGATATGTTGCAATTATCAAAGTTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGttt                                      <  1:1674947/70‑1 (MQ=255)
                                 gCGATATGTTGCAATTATCAAAATTGCTCAGCGTAtt                                                                       >  1:2206936/1‑37 (MQ=255)
                                 gCGATATGTTGCAATTATCAAAATTGCTCAGCGTAtt                                                                       >  1:802392/1‑37 (MQ=255)
                                 gCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGttt                                      <  1:3252420/70‑1 (MQ=255)
                                 gCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGttt                                      <  1:3247130/70‑1 (MQ=255)
                                 gCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGttt                                      <  1:3718858/70‑1 (MQ=255)
                                 gCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGttt                                      <  1:1138087/70‑1 (MQ=255)
                                 gCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTg                                     >  1:2437854/1‑71 (MQ=255)
                                 gCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTg                                     >  1:2904828/1‑71 (MQ=255)
                                   gATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCt                                                            <  1:36939/46‑1 (MQ=255)
                                   gATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCt                                                            <  1:2866789/46‑1 (MQ=255)
                                   gATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCt                                                            <  1:1673822/46‑1 (MQ=255)
                                   gATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCt                                                            <  1:4285634/46‑1 (MQ=255)
                                     tatGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGaa                                            >  1:4260634/1‑60 (MQ=255)
                                      atGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGc                                                             >  1:2476349/1‑42 (MQ=255)
                                      atGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGc                                                             >  1:2611073/1‑42 (MQ=255)
                                      atGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGc                                                             >  1:4180570/1‑42 (MQ=255)
                                        gTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGtt                              >  1:922133/1‑71 (MQ=255)
                                        gTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGtt                              >  1:1462798/1‑71 (MQ=255)
                                         ttGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGtt                              <  1:487457/70‑1 (MQ=255)
                                         ttGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGtt                              <  1:2802024/70‑1 (MQ=255)
                                         ttGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTg                             <  1:3378946/71‑1 (MQ=255)
                                          tGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGaa                                            >  1:571953/1‑55 (MQ=255)
                                          tGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGaa                                            >  1:2567705/1‑55 (MQ=255)
                                          tGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGttt                                      <  1:652564/61‑1 (MQ=255)
                                            cAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCt                                                            <  1:3544557/37‑1 (MQ=255)
                                             aaTTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTgg                                    <  1:3089782/60‑1 (MQ=255)
                                              aTTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGtt                                       <  1:2952674/56‑1 (MQ=255)
                                               ttATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCg                                         <  1:2054037/53‑1 (MQ=255)
                                               ttATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGccc                          >  1:500093/1‑68 (MQ=255)
                                               ttATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCg                         >  1:4112973/1‑69 (MQ=255)
                                                 aTCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGt                                               >  1:1854805/1‑45 (MQ=255)
                                                 aTCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGt                                               >  1:2702532/1‑45 (MQ=255)
                                                 aTCAAAATTGCTCAGCGAATTGGCATTCCGCTGGCGACCATTGGTGAAGCGtt                                       >  1:3025554/1‑53 (MQ=255)
                                                  tCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAgg                     <  1:4009570/70‑1 (MQ=255)
                                                  tCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAgg                     <  1:3315565/70‑1 (MQ=255)
                                                  tCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAgg                     <  1:1572869/70‑1 (MQ=255)
                                                    aaaaTTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAGGGCa                  >  1:2778356/1‑71 (MQ=255)
                                                    aaaaTTGCTCAGCGTATTGGCATTCCGCTGGCGACCAATGGTGAAGc                                          >  1:2256273/1‑47 (MQ=255)
                                                     aaaTTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGt                                        <  1:1397786/48‑1 (MQ=255)
                                                     aaaTTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGt                                        <  1:3309037/48‑1 (MQ=255)
                                                       aTTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGttt                                      >  1:2899979/1‑48 (MQ=255)
                                                          gCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAGGGCATACGtt            <  1:2036813/71‑1 (MQ=255)
                                                          gCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAGGGCATACGtt            <  1:3019254/71‑1 (MQ=255)
                                                           cTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAGGGCATACGtt            >  1:776002/1‑70 (MQ=255)
                                                           cTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAGGGCATACGtt            >  1:4602721/1‑70 (MQ=255)
                                                           cTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAGGGCATACGt             <  1:1315135/69‑1 (MQ=255)
                                                             cAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAGGGCATACGTTAAg         >  1:924809/1‑71 (MQ=255)
                                                                  tATTGGCATTCCGCTGGCGACCATTGGTGAAGCGt                                        <  1:913273/35‑1 (MQ=255)
                                                                  tATTGGCATTCCGCTGGCGACCATTGGTGAAGCGt                                        <  1:1253436/35‑1 (MQ=255)
                                                                  tATTGGCATTCCGCTGGCGACCATTGGTGAAGCGt                                        <  1:195150/35‑1 (MQ=255)
                                                                  tATTGGCATTCCGCTGGCGACCATTGGTGAAGCGt                                        <  1:3417716/35‑1 (MQ=255)
                                                                    ttGGCATTCCGCTGGCGACCATTGGTGAAGCGttt                                      >  1:379828/1‑35 (MQ=255)
                                                                    ttGGCATTCCGCTGGCGACCATTGGTGAAGCGttt                                      >  1:3286082/1‑35 (MQ=255)
                                                                    ttGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAGGGCATACGTTAAGTGCGaaa  <  1:1597260/71‑1 (MQ=255)
                                                                    ttGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAGGGCATACGTTAAGTGCGaaa  <  1:4388410/71‑1 (MQ=255)
                                                                    ttGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAGGGCATACGTTAAGTGCGa    >  1:4401115/1‑69 (MQ=255)
                                                                    ttGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAGGGCATACGTTAAGTGCGa    >  1:1860263/1‑69 (MQ=255)
                                                                    ttGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAGGGCATACGTTAAGTGCGa    >  1:9576/1‑69 (MQ=255)
                                                                    ttGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAGGGCATACGTTAAGTGCGa    >  1:3171896/1‑69 (MQ=255)
                                                                    ttGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAGGGCATACGATAAGTGCGa    >  1:386266/1‑69 (MQ=255)
                                                                    ttGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCACGAAGGGCATACGTTAAGTGCGa    >  1:1538908/1‑69 (MQ=255)
                                                                    |                                                                      
CGGCAATCAGCGGCGATATAAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCGCTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAGGGCATACGTTAAGTGCGAAA  >  Exported/2816471‑2816609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: