| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,736,671 | T→C | 8.4% | F605F (TTT→TTC) | fusA → | protein chain elongation factor EF‑G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,736,671 | 0 | T | C | 8.4% | 238.2 / 11.6 | 83 | F605F (TTT→TTC) | fusA | protein chain elongation factor EF‑G |
| Reads supporting (aligned to +/- strand): ref base T (23/53); new base C (3/4); total (26/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01 | |||||||||||
TTACCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAAGAGAACACCG > Exported/2736602‑2736741 | ttACCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCttt > 1:4300890/1‑70 (MQ=255)ttACCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCttt > 1:2120818/1‑70 (MQ=255) aCCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCttt < 1:3874970/68‑1 (MQ=255) aCCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCttt < 1:3426484/68‑1 (MQ=255) aCCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCttt > 1:3229698/1‑68 (MQ=255) aCCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTaa < 1:4321569/70‑1 (MQ=255) ccATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAg < 1:3349788/70‑1 (MQ=255) ccATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAg < 1:3344185/70‑1 (MQ=255) ccATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGa < 1:4458965/71‑1 (MQ=255) ccATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGa < 1:1807308/71‑1 (MQ=255) aTGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGa > 1:3871842/1‑69 (MQ=255) aTGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGa > 1:740451/1‑69 (MQ=255) aCGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGc < 1:1230447/70‑1 (MQ=255) aCGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGc < 1:3985811/70‑1 (MQ=255) aCGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGc < 1:3034790/70‑1 (MQ=255) aCGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGc < 1:4682761/70‑1 (MQ=255) cGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGa < 1:4714735/71‑1 (MQ=255) cGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGa < 1:4311322/71‑1 (MQ=255) gTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGaa < 1:720092/71‑1 (MQ=255) gTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGaa < 1:447230/71‑1 (MQ=255) gTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGaa < 1:292611/71‑1 (MQ=255) gTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGaa < 1:3346732/71‑1 (MQ=255) gTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGaa < 1:2708253/71‑1 (MQ=255) tGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGaaa > 1:2886503/1‑70 (MQ=255) aCTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAAcc < 1:4314950/70‑1 (MQ=255) aCTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAAcc < 1:568557/70‑1 (MQ=255) aCTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAAcc < 1:3162147/70‑1 (MQ=255) ctcctcTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAACCGAAACCAg < 1:3984118/71‑1 (MQ=255) ctctGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGaaa > 1:3002507/1‑64 (MQ=255) ctctGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGtt < 1:578947/70‑1 (MQ=255) ctctGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGtt < 1:852777/70‑1 (MQ=255) ctctGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTc > 1:1323040/1‑71 (MQ=255) ctctGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTc > 1:4363191/1‑71 (MQ=255) ctctGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTc > 1:762084/1‑71 (MQ=255) ctctGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTc > 1:2605767/1‑71 (MQ=255) tGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCt < 1:1719994/69‑1 (MQ=255) tGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCt < 1:4293002/69‑1 (MQ=255) tGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCt < 1:4287273/69‑1 (MQ=255) aaCTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTg < 1:4361452/68‑1 (MQ=255) aaCTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCtt > 1:1273874/1‑71 (MQ=255) cTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGa < 1:4200285/71‑1 (MQ=255) tGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAg < 1:3714356/47‑1 (MQ=255) gTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGa < 1:2779037/71‑1 (MQ=255) gTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGa < 1:2488361/71‑1 (MQ=255) tAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGaa > 1:4272651/1‑42 (MQ=255) tAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGtt > 1:3205655/1‑55 (MQ=255) tAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCa > 1:843536/1‑71 (MQ=255) tAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCa > 1:2101494/1‑71 (MQ=255) tAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCa > 1:1740863/1‑71 (MQ=255) aaaCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCa > 1:753114/1‑70 (MQ=255) aaaCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCa < 1:2937360/70‑1 (MQ=255) aaCTGGCTGCTTCTATCGCCTTTAAAGAAGGCttt < 1:2694961/35‑1 (MQ=255) aaCTGGCTGCTTCTATCGCCTTTAAAGAAGGCttt < 1:4132566/35‑1 (MQ=255) aaCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCAt < 1:1098071/70‑1 (MQ=255) cTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGaa < 1:1697784/71‑1 (MQ=255) tGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAAcc < 1:1948813/46‑1 (MQ=255) tgctTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTg < 1:3752490/53‑1 (MQ=255) tgctTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTg < 1:3813371/53‑1 (MQ=255) tgctTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTg < 1:2075062/53‑1 (MQ=255) tgctTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTg < 1:4614000/53‑1 (MQ=255) tgctTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGtt < 1:1070733/70‑1 (MQ=255) ctTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAg > 1:3108033/1‑53 (MQ=255) ctTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAg > 1:1761220/1‑53 (MQ=255) ctTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGa < 1:2321089/70‑1 (MQ=255) ttCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGa < 1:3877872/62‑1 (MQ=255) tCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGa < 1:4719035/70‑1 (MQ=255) tCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGa < 1:440015/70‑1 (MQ=255) tCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGa < 1:3582854/70‑1 (MQ=255) tCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGa < 1:1806315/70‑1 (MQ=255) tCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGa < 1:1382490/70‑1 (MQ=255) tCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGa < 1:3721262/70‑1 (MQ=255) tCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGa < 1:1260124/70‑1 (MQ=255) cTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTc > 1:312741/1‑71 (MQ=255) tttAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTg < 1:3366362/36‑1 (MQ=255) tttAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTg < 1:3018796/36‑1 (MQ=255) ttAATGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATg < 1:761983/50‑1 (MQ=255) tAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATc > 1:3732437/1‑46 (MQ=255) tAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATc > 1:938806/1‑46 (MQ=255) tAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCg > 1:3622385/1‑70 (MQ=255) tAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCg > 1:598413/1‑70 (MQ=255) tAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCt > 1:168639/1‑36 (MQ=255) tAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCAGATCATGAAGGTTGAAGTAGAAACTCCg > 1:3918212/1‑70 (MQ=255) aagaagGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGaa < 1:2766499/71‑1 (MQ=255) aagaagGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGa < 1:2652365/70‑1 (MQ=255) aagGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAagag < 1:200647/71‑1 (MQ=255) agGCTTTAAGGAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAagag > 1:4735057/1‑70 (MQ=255) agGCTTTAAGATAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAAgaga < 1:2364191/71‑1 (MQ=255) ggCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAAGAGaa < 1:1940741/71‑1 (MQ=255) ggCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAAGAGaa < 1:389187/71‑1 (MQ=255) gCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAAGAGaa > 1:914395/1‑70 (MQ=255) ttAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCAt < 1:635194/37‑1 (MQ=255) ttAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCAt < 1:4387584/37‑1 (MQ=255) tAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAAgaga > 1:1939508/1‑65 (MQ=255) tAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAAgaga > 1:3434812/1‑65 (MQ=255) tAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAAGAGAACACCg > 1:4505394/1‑71 (MQ=255) | TTACCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAAGAGAACACCG > Exported/2736602‑2736741 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |