Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,469,966 T→G 5.2% L316L (CTT→CTG atpD → F1 sector of membrane‑bound ATP synthase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,469,9660TG 5.2% 320.5 / 9.7 113L316L (CTT→CTGatpDF1 sector of membrane‑bound ATP synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base T (52/55);  new base G (3/3);  total (55/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

CGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACT  >  Exported/2469897‑2470036
                                                                     |                                                                      
cGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCtt                                                                        <  1:4695446/70‑1 (MQ=255)
cGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCtt                                                                        <  1:2507710/70‑1 (MQ=255)
cGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTg                                                                       <  1:900081/71‑1 (MQ=255)
cGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTg                                                                       <  1:1762413/71‑1 (MQ=255)
cGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTg                                                                       <  1:684441/71‑1 (MQ=255)
cGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTg                                                                       <  1:2455939/71‑1 (MQ=255)
  tACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTg                                                                       <  1:3079755/69‑1 (MQ=255)
    cAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGc                                                                   <  1:2636249/71‑1 (MQ=255)
      ggCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGc                                                                   <  1:2425040/69‑1 (MQ=255)
        cAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCaa                                                                 >  1:3788843/1‑69 (MQ=255)
          gTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGt                                                             <  1:1179935/71‑1 (MQ=255)
             taCGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTgg                                                           >  1:849751/1‑70 (MQ=255)
             taCGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTgg                                                           <  1:182821/70‑1 (MQ=255)
             taCGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTgg                                                           >  1:2542183/1‑70 (MQ=255)
             taCGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTgg                                                           >  1:1411898/1‑70 (MQ=255)
             taCGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTgg                                                           <  1:3630071/70‑1 (MQ=255)
             taCGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGt                                                          <  1:4399372/71‑1 (MQ=255)
              aCGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTa                                                         >  1:35067/1‑71 (MQ=255)
               cGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTa                                                         <  1:1592144/70‑1 (MQ=255)
                      gCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGAc                                                                     >  1:3113256/1‑51 (MQ=255)
                      gCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGAc                                                                     >  1:4192641/1‑51 (MQ=255)
                      gCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGAc                                                                     >  1:3124239/1‑51 (MQ=255)
                       cGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGAc                                                                     >  1:2312120/1‑50 (MQ=255)
                       cGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGt                                                >  1:909841/1‑71 (MQ=255)
                         gATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGt                                                >  1:1188678/1‑69 (MQ=255)
                         gATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCa                                              <  1:3408123/71‑1 (MQ=255)
                          aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACTCAACCGTGGTACTGAGCCGTCAg                                             <  1:165944/71‑1 (MQ=255)
                          aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGt                                                >  1:4318262/1‑68 (MQ=255)
                          aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                             <  1:2933339/71‑1 (MQ=255)
                          aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                             <  1:132014/71‑1 (MQ=255)
                          aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                             <  1:4146390/71‑1 (MQ=255)
                          aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                             <  1:3786272/71‑1 (MQ=255)
                           tgacttgactGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                             <  1:3659978/70‑1 (MQ=255)
                            gacttggctGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGt                                                >  1:607228/1‑66 (MQ=255)
                              cttgactGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATcg                                         >  1:4001699/1‑71 (MQ=255)
                               ttgactGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTg                                                            >  1:3431600/1‑51 (MQ=255)
                               ttgactGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATcgc                                        >  1:3997982/1‑71 (MQ=255)
                                   ctgacCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGtct                                    <  1:3506926/71‑1 (MQ=255)
                                      acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                  <  1:1611013/70‑1 (MQ=255)
                                      acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                  <  1:1752367/70‑1 (MQ=255)
                                      acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                  <  1:2921162/70‑1 (MQ=255)
                                      acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                 >  1:2792014/1‑71 (MQ=255)
                                         cGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGt                              <  1:908250/71‑1 (MQ=255)
                                         cGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGt                              <  1:2862459/71‑1 (MQ=255)
                                             tcCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTAt                            >  1:3825130/1‑69 (MQ=255)
                                             tcCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATc                           >  1:4016342/1‑70 (MQ=255)
                                             tcCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATc                           >  1:4189031/1‑70 (MQ=255)
                                               cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGAt                                           >  1:2130285/1‑52 (MQ=255)
                                               cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGAt                                           >  1:3128941/1‑52 (MQ=255)
                                               cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATcgc                                        >  1:376330/1‑55 (MQ=255)
                                               cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATcgc                                        >  1:4562971/1‑55 (MQ=255)
                                               cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                         <  1:869574/70‑1 (MQ=255)
                                               cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                         <  1:2598282/70‑1 (MQ=255)
                                               cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                         <  1:3438039/70‑1 (MQ=255)
                                                ggCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                         <  1:244764/69‑1 (MQ=255)
                                                 gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                 <  1:4256067/60‑1 (MQ=255)
                                                 gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                 <  1:3428217/60‑1 (MQ=255)
                                                 gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAccc                      <  1:4720782/71‑1 (MQ=255)
                                                 gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAccc                      <  1:105299/71‑1 (MQ=255)
                                                  cAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCg                     <  1:3161384/71‑1 (MQ=255)
                                                  cAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCg                     <  1:4189578/71‑1 (MQ=255)
                                                   aaCCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCgg                    >  1:4063829/1‑71 (MQ=255)
                                                      caccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCg                 >  1:735537/1‑71 (MQ=255)
                                                      caccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCg                 >  1:2829053/1‑71 (MQ=255)
                                                      caccTTCGCCCACCTGGACTCGACCGTCGTTCTGTCGCGTGACATCGCATCGCTGGGTATCTACCCTGcgg                 >  1:2501833/1‑69 (MQ=255)
                                                       accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCg                                                 >  1:3440109/1‑38 (MQ=255)
                                                       accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGt                                                >  1:1557602/1‑39 (MQ=255)
                                                       accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGt                                                >  1:463845/1‑39 (MQ=255)
                                                       accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTAt                            >  1:2342622/1‑59 (MQ=255)
                                                       accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCTGGCCGt                <  1:1108/71‑1 (MQ=255)
                                                        ccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGtt               >  1:1017555/1‑71 (MQ=255)
                                                        ccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGtt               <  1:3585632/71‑1 (MQ=255)
                                                        ccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTATGGGTATCTACCCGGCCGtt               <  1:1707410/71‑1 (MQ=255)
                                                         cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAc                        >  1:426069/1‑61 (MQ=255)
                                                         cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTg              <  1:2410584/71‑1 (MQ=255)
                                                         cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATATAc                        >  1:1254073/1‑61 (MQ=255)
                                                           ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGt                                      >  1:25921/1‑45 (MQ=255)
                                                           ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGt                                      >  1:511006/1‑45 (MQ=255)
                                                           ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAc            >  1:3774609/1‑71 (MQ=255)
                                                           ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAc            >  1:2247333/1‑71 (MQ=255)
                                                            tGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCt                          >  1:1432523/1‑56 (MQ=255)
                                                            tGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAcc           >  1:2835828/1‑71 (MQ=255)
                                                            tGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAcc           >  1:3236522/1‑71 (MQ=255)
                                                             gcgcACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAcc           <  1:2577269/70‑1 (MQ=255)
                                                              cgcACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAccc          <  1:3554573/70‑1 (MQ=255)
                                                               ccACCTGGACGCGACCGTCGTACTGTCCCGTGACATCGCCTCGCTGGGTATCTACCCCGCGGTCGACCCGc        <  1:3967805/70‑1 (MQ=255)
                                                               ccACCTGGACGCGACCGTCGTACTGTCCCGTGACATCGCCTCGCTGGGTATCTACCCCGCGGTCGACCCGc        <  1:759270/70‑1 (MQ=255)
                                                                cACCTGGACGCGACCGTCGTACTGTCCCGTGACATCGCCTCGCTGGGTATCTACCCCGCGGTCGACCCGc        <  1:83372/70‑1 (MQ=255)
                                                                 aCCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                  <  1:3237960/43‑1 (MQ=255)
                                                                 aCCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTg      >  1:3383060/1‑71 (MQ=255)
                                                                 aCCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTg      <  1:2977417/71‑1 (MQ=255)
                                                                 aCCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTg      >  1:360219/1‑71 (MQ=255)
                                                                  ccTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCt       >  1:1975031/1‑69 (MQ=255)
                                                                    ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg         >  1:253635/1‑65 (MQ=255)
                                                                    ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg         >  1:684147/1‑65 (MQ=255)
                                                                    ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg         >  1:1802068/1‑65 (MQ=255)
                                                                     tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTa                             >  1:1885760/1‑44 (MQ=255)
                                                                     tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTa                             >  1:2251103/1‑44 (MQ=255)
                                                                     tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACt  >  1:3280172/1‑71 (MQ=255)
                                                                     tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACt  >  1:1984961/1‑71 (MQ=255)
                                                                     |                                                                      
CGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACT  >  Exported/2469897‑2470036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: