| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,134,912 | C→T | 6.9% | L473L (CTG→CTA) | pnp ← | polynucleotide phosphorylase/polyadenylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,134,912 | 0 | C | T | 6.9% | 331.6 / 10.5 | 102 | L473L (CTG→CTA) | pnp | polynucleotide phosphorylase/polyadenylase |
| Reads supporting (aligned to +/- strand): ref base C (57/37); new base T (4/3); total (62/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
TCGCCTTCTTTCACCAGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACAC > Exported/2134844‑2134980 | tCGCCTTCTTTCACCAGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAg > 1:2829889/1‑71 (MQ=255) gCCTTCTTTCACCAGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAg > 1:1293678/1‑69 (MQ=255) cttcttTCACCAGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAgc < 1:3459227/68‑1 (MQ=255) cttcttTCACCAGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGcc < 1:4493474/71‑1 (MQ=255) tcttTCACCAGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAgcgc > 1:5871/1‑68 (MQ=255) cttTCACCAGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCa > 1:776984/1‑69 (MQ=255) tttCACCAGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCa < 1:502094/68‑1 (MQ=255) tCACCAGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCagag < 1:809740/69‑1 (MQ=255) tCACCAGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCaga < 1:4155411/68‑1 (MQ=255) tCACCAGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCaga < 1:2439568/68‑1 (MQ=255) cAGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACgcgc < 1:1989575/71‑1 (MQ=255) cAGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACgcgc < 1:2615983/71‑1 (MQ=255) cAGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACgcgc < 1:3954709/71‑1 (MQ=255) cAGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACgcgc < 1:2258742/71‑1 (MQ=255) cAGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACgcgc < 1:1151621/71‑1 (MQ=255) aGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACgcgc > 1:716693/1‑70 (MQ=255) aGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGcgcc > 1:2900593/1‑71 (MQ=255) aCCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGcgccg > 1:1661077/1‑70 (MQ=255) aCCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGcgccg > 1:2541662/1‑70 (MQ=255) ccATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATTAGCGCCaga < 1:2067357/59‑1 (MQ=255) aTTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATTAGCGCCagag > 1:3732439/1‑58 (MQ=255) aTTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATTAGCGCCAgaga > 1:3993164/1‑59 (MQ=255) aTTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATTAGCGCCAgaga > 1:3991557/1‑59 (MQ=255) aTTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATTAGCGCCAgaga > 1:468228/1‑59 (MQ=255) tGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACg > 1:2569120/1‑70 (MQ=255) tGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACg > 1:3350587/1‑70 (MQ=255) tGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACg > 1:4347131/1‑70 (MQ=255) tGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACg > 1:1076632/1‑70 (MQ=255) tGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACg > 1:1335485/1‑70 (MQ=255) gCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAgaga > 1:1836653/1‑56 (MQ=255) gCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACgg > 1:1513997/1‑70 (MQ=255) aCCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCa > 1:2348635/1‑71 (MQ=255) cGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATag < 1:3539273/71‑1 (MQ=255) gCAACGGCAGCTTTGATCGGCACACCTGCGTCCATGAGCGCCAGAGACGCGCCGCACACGGAAGCCATag > 1:1859000/1‑70 (MQ=255) gCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATag > 1:2535276/1‑70 (MQ=255) aaCGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATagaa < 1:3296134/70‑1 (MQ=255) aaCGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATagaa < 1:736412/70‑1 (MQ=255) aCGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACGCgg > 1:3137850/1‑58 (MQ=255) aCGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACgg > 1:4034679/1‑58 (MQ=255) aCGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACgg > 1:1834080/1‑58 (MQ=255) aCGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACgg > 1:3915019/1‑58 (MQ=255) aCGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACgg > 1:720706/1‑58 (MQ=255) aCGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATagaa > 1:2717552/1‑69 (MQ=255) ggCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGaa > 1:4284733/1‑58 (MQ=255) ggCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAagag > 1:4718190/1‑70 (MQ=255) ggCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAagag > 1:2751763/1‑70 (MQ=255) gCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCAGCACACGGAAGCCATAGAAGAgg > 1:2469745/1‑70 (MQ=255) cAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAagag > 1:3201029/1‑68 (MQ=255) cAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAagag > 1:1107855/1‑68 (MQ=255) cAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAagag > 1:1564410/1‑68 (MQ=255) cAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAagag > 1:1686108/1‑68 (MQ=255) ttGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGtt > 1:3836061/1‑71 (MQ=255) gATCGGCACACCTGCGTCCATCAGCGCCAGAGAcg > 1:440414/1‑35 (MQ=255) gATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACgg > 1:2921776/1‑47 (MQ=255) gATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTg < 1:3922792/70‑1 (MQ=255) gATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTg < 1:1844193/70‑1 (MQ=255) aTCGGCACACCTGCGTCCATTAGCGCCAGAGACGCGCCGCACACGGAAGCCATag < 1:3738072/55‑1 (MQ=255) aTCGGCACACCTGCGTCCATTAGCGCCAGAGACGCGCCGCACACGGAAGCCATag < 1:3534683/55‑1 (MQ=255) aTCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGa < 1:2980266/71‑1 (MQ=255) aTCGGCACACCTGCGTACATCAGCGCCAGAGACGcgccg > 1:1633677/1‑39 (MQ=255) cGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGa > 1:2283269/1‑69 (MQ=255) cGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGAtt > 1:496773/1‑71 (MQ=255) cGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGAtt > 1:3893297/1‑71 (MQ=255) cGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGAtt > 1:897685/1‑71 (MQ=255) cGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGAt > 1:658420/1‑70 (MQ=255) cacaCCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAg < 1:2589368/71‑1 (MQ=255) ccTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGAt > 1:3551647/1‑71 (MQ=255) ccTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGAt > 1:3272807/1‑71 (MQ=255) cTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGAtt > 1:1681715/1‑71 (MQ=255) gCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGAt < 1:2334255/68‑1 (MQ=255) gCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGAt < 1:2456277/68‑1 (MQ=255) cGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGAttt < 1:1053888/69‑1 (MQ=255) cGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGAttt < 1:1408445/69‑1 (MQ=255) cGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGAttt > 1:2480769/1‑69 (MQ=255) cGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGAttt > 1:2490073/1‑69 (MQ=255) cGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGAtt > 1:4597497/1‑68 (MQ=255) cGTCCATCAGCGCCAGAGACGCGACGCACACGGAAGCCATAGAAGAGGAACCGTTCGATTc > 1:1704309/1‑61 (MQ=255) gTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGAttt > 1:2998588/1‑68 (MQ=255) gTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGAttt > 1:2986336/1‑68 (MQ=255) gTCCATCAGCGACAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGAttt > 1:3198040/1‑68 (MQ=255) ccATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAAcc < 1:3693948/49‑1 (MQ=255) ccATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAAcc < 1:4254543/49‑1 (MQ=255) cATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGtt > 1:216448/1‑51 (MQ=255) cATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGtt > 1:1721803/1‑51 (MQ=255) cATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGtt > 1:1373362/1‑51 (MQ=255) aTCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGacac < 1:3776098/71‑1 (MQ=255) tCAGCGCCAGATACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTgg < 1:4399424/51‑1 (MQ=255) tCAGCGCCAGAGACGCGCCGCACACGGAAGCCATataa < 1:881220/38‑1 (MQ=255) tCAGCGCCAGAGACGCGCCGCACACGGAAGCCATagaa < 1:215576/38‑1 (MQ=255) tCAGCGCCAGAGACGCGCCGCACACGGAAGCCATagaa < 1:1396413/38‑1 (MQ=255) tCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTgg < 1:2431585/51‑1 (MQ=255) tCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTgg < 1:3213773/51‑1 (MQ=255) tCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTgg < 1:3741096/51‑1 (MQ=255) tCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTc < 1:3537955/55‑1 (MQ=255) tCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTc < 1:1257595/55‑1 (MQ=255) tCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTc < 1:1665529/55‑1 (MQ=255) tCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTc < 1:4513944/55‑1 (MQ=255) tCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTc < 1:4405626/55‑1 (MQ=255) tCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGacac > 1:2077339/1‑70 (MQ=255) tCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGaca > 1:857499/1‑69 (MQ=255) | TCGCCTTCTTTCACCAGACCCATTGCGATACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACAC > Exported/2134844‑2134980 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |