Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,087,666 C→T 7.9% T54I (ACC→ATC)  yhaH → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,087,6660CT 7.9% 280.3 / 11.1 88T54I (ACC→ATC) yhaHpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (42/39);  new base T (2/5);  total (44/44)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

CATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGAC  >  Exported/2087598‑2087736
                                                                    |                                                                      
catcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCAccc                                                                      >  1:209911/1‑71 (MQ=255)
 atcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCt                                                                     >  1:4109515/1‑71 (MQ=255)
 atcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCt                                                                     >  1:4110018/1‑71 (MQ=255)
 atcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCt                                                                     >  1:2660063/1‑71 (MQ=255)
    tttACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTga                                                                   >  1:3877361/1‑70 (MQ=255)
     ttACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTgac                                                                  >  1:2751383/1‑70 (MQ=255)
         gTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACaa                                                             <  1:4541178/71‑1 (MQ=255)
             gTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTa                                                         <  1:2271480/71‑1 (MQ=255)
             gTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTa                                                         <  1:2797328/71‑1 (MQ=255)
             gTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTa                                                         <  1:1252847/71‑1 (MQ=255)
                cTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACgg                                                      >  1:3783615/1‑71 (MQ=255)
                cTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACg                                                       <  1:2277198/70‑1 (MQ=255)
                 tGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACgg                                                      >  1:2394406/1‑70 (MQ=255)
                  ggggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTgacga                                                                <  1:3870642/59‑1 (MQ=255)
                  ggggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTgacga                                                                <  1:671823/59‑1 (MQ=255)
                  ggggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTgacga                                                                <  1:225163/59‑1 (MQ=255)
                         ctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTg                                               <  1:1826733/69‑1 (MQ=255)
                          tgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGgtg                                            >  1:976975/1‑71 (MQ=255)
                          tgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGgtg                                            >  1:2116584/1‑71 (MQ=255)
                          tgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGgtg                                            >  1:2334108/1‑71 (MQ=255)
                           ggATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGgtgt                                           >  1:844064/1‑71 (MQ=255)
                           ggATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGgtgt                                           >  1:3194604/1‑71 (MQ=255)
                            gATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGgtg                                            <  1:3927312/69‑1 (MQ=255)
                            gATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGtt                                          >  1:3477853/1‑71 (MQ=255)
                            gATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGtt                                          >  1:2138173/1‑71 (MQ=255)
                            gATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGtt                                          >  1:2209407/1‑71 (MQ=255)
                            gATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGtt                                          >  1:4666240/1‑71 (MQ=255)
                            gATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGtt                                          >  1:7018/1‑71 (MQ=255)
                            gATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGtt                                          >  1:1274630/1‑71 (MQ=255)
                             aTAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGttt                                         >  1:4285778/1‑71 (MQ=255)
                             aTAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGttt                                         >  1:1368667/1‑71 (MQ=255)
                              tAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACg                                                       <  1:3950931/56‑1 (MQ=255)
                              tAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGgt                                             >  1:3957770/1‑66 (MQ=255)
                              tAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGtttt                                        >  1:3966253/1‑71 (MQ=255)
                               aaaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTAtt                                                  >  1:998791/1‑60 (MQ=255)
                                aaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTa                                      <  1:4049482/71‑1 (MQ=255)
                                aaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTa                                      >  1:1128834/1‑71 (MQ=255)
                                aaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTa                                      >  1:4168397/1‑71 (MQ=255)
                                aaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTa                                      >  1:4413902/1‑71 (MQ=255)
                                aaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTa                                      >  1:4464547/1‑71 (MQ=255)
                                 aaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTa                                      <  1:1475779/70‑1 (MQ=255)
                                 aaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTAc                                     >  1:897821/1‑71 (MQ=255)
                                   tGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCg                                   <  1:831844/71‑1 (MQ=255)
                                   tGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCg                                   >  1:4334503/1‑71 (MQ=255)
                                   tGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCg                                   >  1:2051148/1‑71 (MQ=255)
                                   tGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCg                                   <  1:981330/71‑1 (MQ=255)
                                   tGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCg                                   <  1:3715899/71‑1 (MQ=255)
                                   tGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTGTTACCg                                   <  1:609614/71‑1 (MQ=255)
                                   tGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCg                                   <  1:3126891/71‑1 (MQ=255)
                                   tGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCg                                   >  1:2907435/1‑71 (MQ=255)
                                   tGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCg                                   <  1:4306694/71‑1 (MQ=255)
                                   tGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCg                                   <  1:3266463/71‑1 (MQ=255)
                                    gTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTGTTACCg                                   <  1:615205/70‑1 (MQ=255)
                                     ttAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACTGTATTCTGGTGTTTTTACCgt                                  <  1:806167/70‑1 (MQ=255)
                                     ttAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgtg                                 >  1:4046478/1‑71 (MQ=255)
                                     ttAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgt                                  <  1:1282706/70‑1 (MQ=255)
                                     ttAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgt                                  <  1:1662235/70‑1 (MQ=255)
                                     ttAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgt                                  <  1:3196608/70‑1 (MQ=255)
                                     ttAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgt                                  <  1:937235/70‑1 (MQ=255)
                                        ggCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgtggtg                              <  1:3959305/71‑1 (MQ=255)
                                        ggCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgtggtg                              <  1:3986970/71‑1 (MQ=255)
                                         gCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgtggtg                              <  1:1241270/70‑1 (MQ=255)
                                          cTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGtggtgg                             >  1:330446/1‑70 (MQ=255)
                                          cTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTggg                            <  1:1358533/71‑1 (MQ=255)
                                          cTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTggg                            <  1:2725874/71‑1 (MQ=255)
                                             gCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCgg                         <  1:2698851/71‑1 (MQ=255)
                                             gCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCgg                         <  1:1043357/71‑1 (MQ=255)
                                               aaCGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGtt                       >  1:2166033/1‑71 (MQ=255)
                                               aaCGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGtt                       >  1:2706849/1‑71 (MQ=255)
                                                aCGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgtgg                                <  1:4241622/61‑1 (MQ=255)
                                                 cgcgCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTa                                                    <  1:1917592/40‑1 (MQ=255)
                                                 cgcgCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGt                        <  1:4126675/68‑1 (MQ=255)
                                                  gcgcTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAg                    <  1:3941644/71‑1 (MQ=255)
                                                  gcgcTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAg                    <  1:3874144/71‑1 (MQ=255)
                                                   cgcgGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTggg                            <  1:3718039/62‑1 (MQ=255)
                                                    gcTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgt                                  <  1:4296004/55‑1 (MQ=255)
                                                    gcTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGtt                  >  1:4423940/1‑71 (MQ=255)
                                                     cTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTc                 <  1:1629086/71‑1 (MQ=255)
                                                        gtggCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGtt                       >  1:3079401/1‑62 (MQ=255)
                                                        gtggCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTccgc              >  1:4646717/1‑71 (MQ=255)
                                                         tggCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTccgcc             <  1:2760292/71‑1 (MQ=255)
                                                            cGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAg                    >  1:3796016/1‑61 (MQ=255)
                                                            cGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGt                   >  1:4222284/1‑62 (MQ=255)
                                                            cGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGt                   >  1:2938811/1‑62 (MQ=255)
                                                              aaGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTg        <  1:1623125/71‑1 (MQ=255)
                                                               aGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTg        >  1:1812036/1‑70 (MQ=255)
                                                               aGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTg        >  1:2305962/1‑70 (MQ=255)
                                                                ggCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCa      >  1:815361/1‑71 (MQ=255)
                                                                 gCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCAc     <  1:292206/71‑1 (MQ=255)
                                                                    cccTGACGACAATCTACGGTATTCTGGTGTTTTTACCg                                   <  1:1179509/38‑1 (MQ=255)
                                                                    cccTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGac  <  1:1617521/71‑1 (MQ=255)
                                                                    cccTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGac  <  1:3951409/71‑1 (MQ=255)
                                                                    cccTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGac  <  1:898004/71‑1 (MQ=255)
                                                                    |                                                                      
CATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGAC  >  Exported/2087598‑2087736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: