| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,259,237 | G→A | 10.8% | S613F (TCT→TTT) | tdcE ← | pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,259,237 | 0 | G | A | 10.8% | 189.5 / 11.4 | 65 | S613F (TCT→TTT) | tdcE | pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase |
| Reads supporting (aligned to +/- strand): ref base G (28/29); new base A (4/3); total (32/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01 | |||||||||||
CGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTTTCTTCA > AP009048/3259170‑3259306 | cgcgAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAg > 1:2145069/1‑71 (MQ=255)cgcgAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAg > 1:1938242/1‑71 (MQ=255) aCGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGa > 1:808313/1‑69 (MQ=255) cGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATa < 1:2924262/70‑1 (MQ=255) cGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATa < 1:3036283/70‑1 (MQ=255) gtgtTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGt < 1:3034022/70‑1 (MQ=255) ttCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAg > 1:1347236/1‑70 (MQ=255) cGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGaa > 1:3192118/1‑56 (MQ=255) cGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATc < 1:1912871/71‑1 (MQ=255) cGGCGCGAAGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATc > 1:66305/1‑71 (MQ=255) ggCGCTACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCg < 1:807962/71‑1 (MQ=255) ggCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTaaaa > 1:1481/1‑55 (MQ=255) ggCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAg < 1:847858/56‑1 (MQ=255) ggCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAg < 1:2969618/56‑1 (MQ=255) ggCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc > 1:1566787/1‑70 (MQ=255) agcgcGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCg < 1:632086/70‑1 (MQ=255) gcgcGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGa < 1:3432862/58‑1 (MQ=255) gcgACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGa < 1:561125/69‑1 (MQ=255) cgacgaCCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCg < 1:3326378/67‑1 (MQ=255) acgacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTggg < 1:2312995/71‑1 (MQ=255) cgacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGt < 1:347907/71‑1 (MQ=255) gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTa < 1:74401/71‑1 (MQ=255) ccGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAgg > 1:1573275/1‑71 (MQ=255) cGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAgg > 1:4564942/1‑70 (MQ=255) tCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGAc > 1:3334604/1‑71 (MQ=255) ccGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGa > 1:3545860/1‑69 (MQ=255) cGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATc > 1:585849/1‑55 (MQ=255) cGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTggg > 1:980575/1‑62 (MQ=255) cGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACgg > 1:169880/1‑71 (MQ=255) cGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAggg < 1:2377998/64‑1 (MQ=255) cGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAgg > 1:131499/1‑63 (MQ=255) cGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAgg > 1:154810/1‑63 (MQ=255) gTATTACCGGTTTTCTGGCCGTACACCACGTTAGaa < 1:2126616/36‑1 (MQ=255) ttACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGAc < 1:3644380/51‑1 (MQ=255) ttACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCg < 1:155517/71‑1 (MQ=255) aCCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGaa > 1:444483/1‑44 (MQ=255) ccGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATa < 1:184668/71‑1 (MQ=255) ccGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGCTAGGGACGGCGTTGCGATa < 1:3841607/71‑1 (MQ=255) ccGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGAt < 1:2541551/70‑1 (MQ=255) ggTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCg > 1:1419061/1‑61 (MQ=255) gTTTTCTGGCCGTACACCACGTTAGAAGTGATAGGCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGt < 1:611177/71‑1 (MQ=255) ttttCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGtt < 1:711161/71‑1 (MQ=255) ttCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTgg > 1:2725093/1‑71 (MQ=255) tCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTgg > 1:2058033/1‑70 (MQ=255) tCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTgg < 1:2317649/70‑1 (MQ=255) cTGGCCTTACACCACGTTATAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGtt < 1:2717938/67‑1 (MQ=255) gCCGTACACCACGTTAGAAGTCATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCa < 1:4189793/68‑1 (MQ=255) ccGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAgg < 1:3033317/61‑1 (MQ=255) ccGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAgcgc > 1:3315082/1‑71 (MQ=255) cGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAg < 1:1136754/59‑1 (MQ=255) gTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCtt > 1:744044/1‑71 (MQ=255) tACACCACGTTAGAAGTGATAGTCAGAATCGACTg > 1:3443269/1‑35 (MQ=255) caccacGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGAt > 1:693623/1‑53 (MQ=255) caccacGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAg > 1:3065142/1‑63 (MQ=255) caccacGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTaa < 1:1828131/71‑1 (MQ=255) caccacGTTAAAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCt > 1:4113023/1‑67 (MQ=255) ccacGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAAtt < 1:3829206/71‑1 (MQ=255) acGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGtt < 1:3075266/54‑1 (MQ=255) acGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAAttt > 1:3269375/1‑70 (MQ=255) cGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTg > 1:3932308/1‑44 (MQ=255) cGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATa > 1:3773618/1‑49 (MQ=255) cGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATa > 1:3452584/1‑49 (MQ=255) cGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTttc > 1:4602791/1‑71 (MQ=255) gTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTttct < 1:719681/71‑1 (MQ=255) ttAAAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTttctt < 1:1295317/71‑1 (MQ=255) aGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTTTCTTCa > 1:66667/1‑71 (MQ=255) | CGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTTTCTTCA > AP009048/3259170‑3259306 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |