| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 71,175 | A→G | 53.6% | R263R (CGA→CGG) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 71,175 | 0 | A | G | 53.6% | 24.7 / 144.7 | 104 | R263R (CGA→CGG) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base A (25/23); new base G (17/37); total (42/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.45e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.51e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
ACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATG > AP009048/71107‑71242 | accaCCCGGATGCCTATCGCCACCGTCTGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGt < 1:3796978/68‑1 (MQ=255) cTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTa < 1:1602209/71‑1 (MQ=255) aCCCGCATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTAtt < 1:2224324/71‑1 (MQ=255) cccGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTAtt > 1:2657871/1‑70 (MQ=255) ggATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaa > 1:47297/1‑70 (MQ=255) gATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGt < 1:525403/63‑1 (MQ=255) gATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:169640/71‑1 (MQ=255) gATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:3197712/71‑1 (MQ=255) gATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATATCTCGCGAGTATTTaaaa < 1:2879166/71‑1 (MQ=255) aTGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaa < 1:4568978/71‑1 (MQ=255) aTGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaa < 1:3429566/71‑1 (MQ=255) aTGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaaa < 1:3480890/71‑1 (MQ=255) tGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaa < 1:3864020/70‑1 (MQ=255) ccTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAAt < 1:3048957/70‑1 (MQ=255) cTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGa > 1:1530333/1‑56 (MQ=255) cTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGc < 1:4419042/71‑1 (MQ=255) aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTAttt > 1:4512819/1‑60 (MQ=255) aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc > 1:1274030/1‑71 (MQ=255) aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAAt > 1:2239693/1‑67 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAg > 1:524931/1‑54 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCa < 1:2028495/69‑1 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCAcc < 1:1647528/71‑1 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCAcc > 1:1922385/1‑71 (MQ=255) cGCCTCCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaa < 1:3682114/62‑1 (MQ=255) cGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTAttt < 1:2348994/58‑1 (MQ=255) cGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCg > 1:3666338/1‑71 (MQ=255) gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCgg > 1:325033/1‑71 (MQ=255) gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCg < 1:1534979/70‑1 (MQ=255) gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCg < 1:4383261/70‑1 (MQ=255) aCCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGc > 1:179126/1‑71 (MQ=255) ccGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGc > 1:3015720/1‑70 (MQ=255) ccGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGc > 1:366677/1‑70 (MQ=255) ccGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGc > 1:1680508/1‑70 (MQ=255) ccGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGc > 1:1513811/1‑70 (MQ=255) cGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGc > 1:1319405/1‑69 (MQ=255) cGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGc > 1:1047754/1‑69 (MQ=255) gtcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa > 1:4000149/1‑57 (MQ=255) cggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGcc > 1:2800834/1‑71 (MQ=255) cggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCgggg > 1:2026883/1‑65 (MQ=255) cggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGc > 1:2424080/1‑70 (MQ=255) ggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGccc < 1:3742700/71‑1 (MQ=255) ggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGcc < 1:3373958/70‑1 (MQ=255) gtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa < 1:1895163/53‑1 (MQ=255) gtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa < 1:4503320/49‑1 (MQ=255) tcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTAttt < 1:2637072/46‑1 (MQ=255) tcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcga < 1:2795500/71‑1 (MQ=255) gCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGa > 1:2126366/1‑38 (MQ=255) gCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagc > 1:510054/1‑71 (MQ=255) gCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCcgagc > 1:2283960/1‑71 (MQ=255) gCAATGTTGGTTTTGACGATCAACTATATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCcgagc < 1:2515817/71‑1 (MQ=255) cAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCAAGCCcg < 1:4218282/67‑1 (MQ=255) aaTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa < 1:1342952/48‑1 (MQ=255) aaTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcg < 1:4090607/66‑1 (MQ=255) aTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGt < 1:1043224/37‑1 (MQ=255) aTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGt < 1:1002516/37‑1 (MQ=255) aTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa < 1:454463/43‑1 (MQ=255) aTGTTGG‑TTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGt < 1:4040406/71‑1 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGc > 1:100372/1‑61 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGt > 1:3527487/1‑71 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGt < 1:2198723/71‑1 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGt < 1:1760413/71‑1 (MQ=255) gTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:3784517/43‑1 (MQ=255) gTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaa < 1:354433/42‑1 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:733057/42‑1 (MQ=255) ttGGTTTTGACGATCAACTCTATTTATCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGtt < 1:795184/70‑1 (MQ=255) tGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATg < 1:2078041/45‑1 (MQ=255) ggTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttcc < 1:3998259/70‑3 (MQ=255) ggTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttcc < 1:491349/70‑3 (MQ=255) ggTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCg > 1:2528592/1‑71 (MQ=255) ttttGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCggg < 1:4493175/49‑1 (MQ=255) ttttGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGt > 1:1190881/1‑70 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGcc > 1:4264195/1‑71 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGcc > 1:1142817/1‑71 (MQ=255) ttGACGATCAACTCTATTTCTCGCGAGTATTTaaaaaa > 1:641183/1‑38 (MQ=255) ttGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGcc > 1:3639008/1‑71 (MQ=255) ttGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGc < 1:2561113/70‑1 (MQ=255) tGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGcc < 1:1894802/70‑1 (MQ=255) gACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCgg > 1:3893690/1‑71 (MQ=255) cGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGtt < 1:2463305/71‑1 (MQ=255) gATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcg < 1:2903747/51‑1 (MQ=255) aTCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgt < 1:515647/71‑1 (MQ=255) aTCAACTCTATTTCTCGCG‑GTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg < 1:1211173/71‑1 (MQ=255) aCTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCgg < 1:19709/63‑1 (MQ=255) tctATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTgaaga > 1:2458288/1‑70 (MQ=255) ctATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTgaa > 1:438859/1‑67 (MQ=255) tATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaagaa < 1:1419445/69‑1 (MQ=255) tATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaagaa < 1:1360350/69‑1 (MQ=255) tATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa > 1:3086791/1‑70 (MQ=255) tATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa > 1:3149851/1‑70 (MQ=255) aTTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGtt > 1:3887558/1‑49 (MQ=255) tttCTCGCGGGTATTTAAATAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaaa < 1:563159/69‑1 (MQ=255) tttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaaa < 1:3958265/69‑1 (MQ=255) tttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAg > 1:4480953/1‑71 (MQ=255) ttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGt > 1:3502836/1‑71 (MQ=255) tctcGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGt < 1:2687299/70‑1 (MQ=255) tctcGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGt > 1:2275071/1‑70 (MQ=255) ctcGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTg > 1:3038363/1‑70 (MQ=255) gcgTGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAat < 1:3895845/70‑1 (MQ=255) gcgAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:2675538/71‑1 (MQ=255) gcgAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:3578753/71‑1 (MQ=255) | ACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATG > AP009048/71107‑71242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |