| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 2,622,878 | T→C | 6.5% | G393G (GGT→GGC) | ppk → | polyphosphate kinase, component of RNA degradosome |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 2,622,878 | 0 | T | C | 6.5% | 213.5 / 5.2 | 76 | G393G (GGT→GGC) | ppk | polyphosphate kinase, component of RNA degradosome |
| Reads supporting (aligned to +/- strand): ref base T (36/35); new base C (3/2); total (39/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGC > AP009048/2622813‑2622948 | aaCATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTGGATGATCCACGCCGCACATAACGGTAAGa < 1:981944/70‑1 (MQ=255) tACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCa < 1:4145235/70‑1 (MQ=255) tACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCa < 1:3419858/70‑1 (MQ=255) tACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCa < 1:1126552/70‑1 (MQ=255) cgcgTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgt > 1:434913/1‑71 (MQ=255) gcgTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtg > 1:2657853/1‑71 (MQ=255) gTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtggt < 1:1654729/71‑1 (MQ=255) gCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGaa > 1:3769428/1‑56 (MQ=255) gCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGtggtgg > 1:985761/1‑70 (MQ=255) gCGAAAGATTCACGCATCATCGACTCGATGATCCACGC‑‑CACATAACGGTAAGAAAGTCACCGTGGTGGTTg < 1:812176/71‑1 (MQ=255) cGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGaaa < 1:566251/56‑1 (MQ=255) cGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGtt < 1:1483402/71‑1 (MQ=255) cGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGtt < 1:519875/71‑1 (MQ=255) gAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTg < 1:527807/71‑1 (MQ=255) gAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTg < 1:3393536/71‑1 (MQ=255) aaaGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGtt < 1:3082607/69‑1 (MQ=255) aaaGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGtt > 1:672753/1‑69 (MQ=255) aaGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAg > 1:1319059/1‑71 (MQ=255) aaGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAg < 1:2550496/71‑1 (MQ=255) gATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGtt < 1:3944196/71‑1 (MQ=255) gATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGtt < 1:4126685/71‑1 (MQ=255) gATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGt > 1:3627821/1‑70 (MQ=255) aTTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTa > 1:186345/1‑71 (MQ=255) ttCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTAc < 1:1765473/71‑1 (MQ=255) aCGCATCATCGACTCGATGATCCACGCCGCACATAACGGCAAGaaa < 1:2531644/46‑1 (MQ=255) cGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAgg > 1:3083925/1‑70 (MQ=255) cGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGgc < 1:3224352/71‑1 (MQ=255) catcatCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGtt < 1:3144375/58‑1 (MQ=255) atcatcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTc < 1:654207/45‑1 (MQ=255) atcatcGACTCGATGATCCACGCCGCACATAACGGCAAGAAAGTCACCGTGGTGGTTGAGTTACAGGcgcg > 1:3373837/1‑71 (MQ=255) tcatcGACTCGATGATCCACGCCGCACATAACGGTAAGa < 1:1482925/39‑1 (MQ=255) tcatcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTg < 1:3474608/57‑1 (MQ=255) atcGACTCGATGATCCACGCCTCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGttt > 1:1964970/1‑71 (MQ=255) atcGACTCGATGATCCACGCCGCACATAACGGTAAGaaa > 1:1405025/1‑39 (MQ=255) atcGACTCGATGATCCACGCCGCACATAACGGTAAGaaa > 1:2571132/1‑39 (MQ=255) atcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGtt > 1:774663/1‑70 (MQ=255) atcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGt > 1:4099070/1‑69 (MQ=255) tcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTc < 1:717522/71‑1 (MQ=255) tcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTc < 1:3638312/71‑1 (MQ=255) cGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTc > 1:2484083/1‑40 (MQ=255) cGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGtt > 1:4009178/1‑52 (MQ=255) gACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtgg > 1:203430/1‑46 (MQ=255) cTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTcga > 1:4140114/1‑69 (MQ=255) tCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTcgacg > 1:87025/1‑70 (MQ=255) cGATGATCCACGCCGCACATAACGGTAAGAAAGTCa > 1:4191543/1‑36 (MQ=255) cGATGATCCACGCCGCACATAACGGCAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTcgacg > 1:1332325/1‑69 (MQ=255) gatgatCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGtt < 1:4159446/51‑1 (MQ=255) gatgatCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTcgacg > 1:1599854/1‑68 (MQ=255) gatgatCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTcgacg > 1:2156042/1‑68 (MQ=255) gatgatCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTcgacg > 1:3997364/1‑68 (MQ=255) tgatCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACa > 1:865860/1‑52 (MQ=255) tgatCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACa > 1:610868/1‑52 (MQ=255) tgatCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAgg > 1:4169358/1‑54 (MQ=255) atCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGc < 1:487384/71‑1 (MQ=255) tCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGcc < 1:2028376/71‑1 (MQ=255) tCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGcc < 1:1219279/71‑1 (MQ=255) tCCACGCCGCACATAACGGCAAGAAAGTCACCGTGGTGGTTGa < 1:567371/43‑1 (MQ=255) ccACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGcc > 1:2642849/1‑70 (MQ=255) ccACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGcc < 1:776144/70‑1 (MQ=255) aCGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTa < 1:4084732/44‑1 (MQ=255) cgccgcACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTAc > 1:3493584/1‑44 (MQ=255) gccgcACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACAt > 1:1718043/1‑71 (MQ=255) ccgcACATAACGGTAAGAAAGTCACCGTGGTGGtt > 1:1325362/1‑35 (MQ=255) cacaTAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACgaagaa < 1:970394/59‑1 (MQ=255) acaTAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTAc > 1:1517313/1‑38 (MQ=255) acaTAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACt > 1:3661409/1‑71 (MQ=255) acaTAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACt < 1:2001613/71‑1 (MQ=255) caTAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTa > 1:3810764/1‑36 (MQ=255) caTAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAGCATTCACTg > 1:3514553/1‑71 (MQ=255) caTAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTg < 1:3937120/71‑1 (MQ=255) aTAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTgg < 1:398201/71‑1 (MQ=255) aaCGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGc > 1:1202821/1‑71 (MQ=255) aCGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCg < 1:705039/71‑1 (MQ=255) cGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTagacga > 1:2207292/1‑48 (MQ=255) cGGCAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCg > 1:196462/1‑70 (MQ=255) ggTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGaa < 1:3438430/71‑1 (MQ=255) tAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAgc < 1:2037655/71‑1 (MQ=255) tAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAgc < 1:1228730/71‑1 (MQ=255) | AACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGC > AP009048/2622813‑2622948 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |