| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 2,493,033 | C→T | 6.5% | Q1072* (CAG→TAG) | evgS → | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 2,493,033 | 0 | C | T | 6.5% | 352.5 / 9.6 | 107 | Q1072* (CAG→TAG) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Reads supporting (aligned to +/- strand): ref base C (48/52); new base T (5/2); total (53/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
Only 100 of 112 total aligned reads displayed.
GTTAAGTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATAC > AP009048/2492963‑2493103 | gTTAAGTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTc < 1:386714/71‑1 (MQ=255) aaGTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGt > 1:1088265/1‑71 (MQ=255) gTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTa < 1:2106729/71‑1 (MQ=255) gTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTa > 1:3379399/1‑71 (MQ=255) tGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTac > 1:1100642/1‑70 (MQ=255) gCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTacac > 1:1176961/1‑71 (MQ=255) gCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTa > 1:4119723/1‑68 (MQ=255) cGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACAcc < 1:3823712/71‑1 (MQ=255) cGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACAcc < 1:3543397/71‑1 (MQ=255) cGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACAcc < 1:612312/71‑1 (MQ=255) ggCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACAcc > 1:4252740/1‑70 (MQ=255) cATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCatg < 1:2161322/71‑3 (MQ=255) aTGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGt > 1:65827/1‑71 (MQ=255) tGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAg < 1:2065497/69‑1 (MQ=255) tGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGtt > 1:1014226/1‑71 (MQ=255) aaCTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTgc > 1:648237/1‑71 (MQ=255) ttATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGt < 1:531409/55‑1 (MQ=255) ttATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCa > 1:85931/1‑71 (MQ=255) tATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCa > 1:1038335/1‑70 (MQ=255) aTGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCat < 1:1539234/70‑1 (MQ=255) tgtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCata > 1:2563998/1‑70 (MQ=255) gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGt > 1:666637/1‑51 (MQ=255) gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGt > 1:2482917/1‑51 (MQ=255) tttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATAtt < 1:2641834/70‑1 (MQ=255) ttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTg < 1:1955973/70‑1 (MQ=255) ttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTg > 1:4058725/1‑70 (MQ=255) ttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTg > 1:732548/1‑70 (MQ=255) ttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTg > 1:4087637/1‑70 (MQ=255) tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAg > 1:3248665/1‑47 (MQ=255) tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCa > 1:3836766/1‑71 (MQ=255) tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCa > 1:789093/1‑71 (MQ=255) gttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCa < 1:382205/70‑1 (MQ=255) cAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCt < 1:3000366/70‑1 (MQ=255) cAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCt > 1:2562265/1‑70 (MQ=255) aaaCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTc < 1:305766/70‑1 (MQ=255) aaCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAg < 1:1480215/71‑1 (MQ=255) aaCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAg > 1:1441283/1‑71 (MQ=255) aaCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAg < 1:2167301/71‑1 (MQ=255) aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTTCGCATATTGCACCTCa > 1:3952334/1‑69 (MQ=255) aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGt < 1:3487426/71‑1 (MQ=255) aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGt < 1:3807491/71‑1 (MQ=255) aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGt < 1:3360007/71‑1 (MQ=255) ccGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTac > 1:888821/1‑43 (MQ=255) ccGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCa > 1:1340310/1‑68 (MQ=255) ccGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTa < 1:1513539/71‑1 (MQ=255) ttGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGt < 1:573353/67‑1 (MQ=255) ttGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCg < 1:1679280/71‑1 (MQ=255) tGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGc > 1:216438/1‑71 (MQ=255) aCCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTgc < 1:3992119/47‑1 (MQ=255) aCCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTgc < 1:4178538/47‑1 (MQ=255) aCCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGcc < 1:314650/70‑1 (MQ=255) ccTGGATGTACTGAAAACACATTTACGTCAGTTACACCAAGTTGCGCa < 1:4199467/48‑1 (MQ=255) ccTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCa < 1:4114838/48‑1 (MQ=255) ccTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc > 1:3465375/1‑71 (MQ=255) ccTGCATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc < 1:2455344/71‑1 (MQ=255) cTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATc > 1:2708791/1‑64 (MQ=255) cTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCt < 1:3345208/71‑1 (MQ=255) tGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCa > 1:3709708/1‑53 (MQ=255) tGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCAc > 1:1894278/1‑54 (MQ=255) tGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGc > 1:215241/1‑65 (MQ=255) tGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCt > 1:3393545/1‑70 (MQ=255) ggATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCtt > 1:887177/1‑70 (MQ=255) ggATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCt < 1:1321240/69‑1 (MQ=255) ggATGTACTGAAAACACATTTAAGTCAGTTACACCAAATTGCGCATATTGCAc < 1:1749509/53‑1 (MQ=255) gATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCtt < 1:735618/69‑1 (MQ=255) gATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGa < 1:2848137/71‑1 (MQ=255) tGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACATTGata > 1:780858/1‑71 (MQ=255) gTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCatat < 1:4491376/44‑1 (MQ=255) gTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGatat > 1:2417216/1‑71 (MQ=255) tACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCTCCACCTTGatat < 1:3937813/70‑1 (MQ=255) aCTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATc > 1:1519012/1‑70 (MQ=255) cTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATTTTGCACCTCAGTATCGCCACCtt < 1:4132359/63‑1 (MQ=255) cTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCg < 1:3328856/70‑1 (MQ=255) tGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGc < 1:2990854/43‑1 (MQ=255) gAAAACACATTTAAGTCAGTTACACCAAGTTGCGCa < 1:1236426/36‑1 (MQ=255) gAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATc > 1:4133653/1‑53 (MQ=255) gAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCg > 1:2820661/1‑54 (MQ=255) gAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCg > 1:1634849/1‑54 (MQ=255) aaaaCACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGaa < 1:218868/69‑1 (MQ=255) aaaaCACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGaa < 1:3720856/69‑1 (MQ=255) aaaaCACATTTAAGACAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGaa > 1:2008574/1‑69 (MQ=255) acacATTTAAGTCAGTTACACCAAGTTGCGCATATTg < 1:705979/37‑1 (MQ=255) acaTTTAAGTCAGTTACACCTAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAg < 1:3415133/65‑1 (MQ=255) acaTTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCtt > 1:3550815/1‑55 (MQ=255) acaTTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGcc > 1:320495/1‑67 (MQ=255) acaTTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTg > 1:2376170/1‑70 (MQ=255) aTTTAAGTTAGTTACACCAAGTTGCGCATATTGCAc > 1:1717962/1‑36 (MQ=255) aTTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGaaa < 1:3387971/71‑1 (MQ=255) ttAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGc > 1:2381636/1‑45 (MQ=255) ttAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGc > 1:2386739/1‑45 (MQ=255) tAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCa > 1:2853939/1‑37 (MQ=255) tAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGaaaaa < 1:4509139/70‑1 (MQ=255) tAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGaaaaa > 1:2874684/1‑70 (MQ=255) aaGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGatat > 1:3638225/1‑54 (MQ=255) aaGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGaa > 1:16397/1‑58 (MQ=255) aGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaataa < 1:268219/71‑1 (MQ=255) gTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCtt < 1:2781203/47‑1 (MQ=255) cAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGccc > 1:3291596/1‑58 (MQ=255) cAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGccc > 1:1760519/1‑58 (MQ=255) cAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATAc > 1:1525443/1‑71 (MQ=255) | GTTAAGTTGCGGCATGAACTTATGTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATAC > AP009048/2492963‑2493103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |