| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 1,364,882 | G→A | 5.9% | V142V (GTG→GTA) | puuC → | gamma‑Glu‑gamma‑aminobutyraldehyde dehydrogenase, NAD(P)H‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 1,364,882 | 0 | G | A | 5.9% | 224.7 / 2.9 | 68 | V142V (GTG→GTA) | puuC | gamma‑Glu‑gamma‑aminobutyraldehyde dehydrogenase, NAD(P)H‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (37/27); new base A (2/2); total (39/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01 | |||||||||||
TGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTGCCGCCA > AP009048/1364813‑1364946 | tgatATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTg > 1:3703456/1‑70 (MQ=255) atatTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGc < 1:4391032/70‑1 (MQ=255) tatTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGa > 1:4165981/1‑71 (MQ=255) ttCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGac < 1:1869418/70‑1 (MQ=255) tCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGacca > 1:69444/1‑71 (MQ=255) tCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGacca > 1:3646034/1‑71 (MQ=255) cggcgcggcgCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGaccac > 1:198570/1‑69 (MQ=255) ggcgcggcgcGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGAccaccac > 1:3707965/1‑70 (MQ=255) gcgcggcgcgCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAg > 1:1070406/1‑70 (MQ=255) gcgcggcgcgCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGt < 1:339453/71‑1 (MQ=255) gcgcggcgcgCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGt < 1:2845488/71‑1 (MQ=255) cgcgcgcCATTCGCTTGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCAt < 1:1457736/71‑1 (MQ=255) cgcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCAt > 1:1341225/1‑71 (MQ=255) cgcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCAt < 1:2581137/71‑1 (MQ=255) cgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCAt > 1:2818056/1‑69 (MQ=255) cgcgcCATTCGCTGGTACGCAGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGa < 1:38094/71‑1 (MQ=255) cATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTg > 1:535933/1‑70 (MQ=255) ttCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGac < 1:1086785/49‑1 (MQ=255) ttCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGc > 1:4384397/1‑70 (MQ=255) ttCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGc > 1:1854152/1‑70 (MQ=255) ttCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCg < 1:937086/71‑1 (MQ=255) ttCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCg < 1:1759536/71‑1 (MQ=255) tCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCga > 1:373525/1‑71 (MQ=255) cGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGAccacca > 1:431214/1‑52 (MQ=255) gCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCgat < 1:2054728/70‑1 (MQ=255) gCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCgat > 1:4586267/1‑70 (MQ=255) gCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCgat > 1:2023945/1‑70 (MQ=255) tGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCgatgat > 1:663505/1‑71 (MQ=255) tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATcgt < 1:983435/71‑1 (MQ=255) tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATcgt < 1:555095/71‑1 (MQ=255) cGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATcgtgc > 1:4231604/1‑71 (MQ=255) ccGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGc < 1:4059824/47‑1 (MQ=255) ccGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCAt < 1:3980730/50‑1 (MQ=255) ccGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATcgtgcgt > 1:2582483/1‑71 (MQ=255) gAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATcgtgcgtg > 1:3590011/1‑70 (MQ=255) aGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAAc > 1:4289279/1‑71 (MQ=255) aGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAAc > 1:4284030/1‑71 (MQ=255) aGCGATCGACAAAGTGTATGGCCAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAAc < 1:1345343/71‑1 (MQ=255) gCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCgatg < 1:118976/57‑1 (MQ=255) gATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCt < 1:3255352/48‑1 (MQ=255) aTCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCGATGATcgtg < 1:1189815/60‑1 (MQ=255) tCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGt > 1:3879616/1‑70 (MQ=255) cGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTc > 1:3218741/1‑70 (MQ=255) cGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCg > 1:1744948/1‑71 (MQ=255) cGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCg > 1:3643089/1‑71 (MQ=255) gACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAg > 1:1487074/1‑42 (MQ=255) gACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGt < 1:4519072/68‑1 (MQ=255) gACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCgg < 1:1225278/71‑1 (MQ=255) aCAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATcgtg > 1:890369/1‑56 (MQ=255) aCAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGc < 1:1596907/71‑1 (MQ=255) cAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGa < 1:1653350/60‑1 (MQ=255) cAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCg > 1:3584054/1‑71 (MQ=255) cAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCg > 1:2973669/1‑71 (MQ=255) aaaGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCg > 1:3017239/1‑63 (MQ=255) aaaGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCTGCGt < 1:817956/71‑1 (MQ=255) aaaGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGt > 1:2333458/1‑71 (MQ=255) gtgtATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGAt > 1:801925/1‑71 (MQ=255) tgtATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGAtt < 1:3550479/71‑1 (MQ=255) gtATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCgatga < 1:87913/44‑1 (MQ=255) aTGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTgcc > 1:1898447/1‑71 (MQ=255) tGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATcgtg > 1:2606887/1‑46 (MQ=255) tGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTTCGTGAACCGGTCGGCGTGATTgccg > 1:2355535/1‑71 (MQ=255) gCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGAt > 1:3517486/1‑64 (MQ=255) gCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTgccgc > 1:685345/1‑70 (MQ=255) gCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTgccgc > 1:318675/1‑70 (MQ=255) cGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTGCCGCCa > 1:2063246/1‑71 (MQ=255) cGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTGCCGCCa < 1:4274172/71‑1 (MQ=255) tGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTgcc < 1:246374/62‑1 (MQ=255) tGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTg < 1:1807882/60‑1 (MQ=255) | TGATATTCCCGGCGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGTCGGCGTGATTGCCGCCA > AP009048/1364813‑1364946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |