| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 1,255,918 | C→T | 12.5% | G538S (GGC→AGC) | ycgV ← | predicted adhesin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 1,255,918 | 0 | C | T | 12.5% | 132.3 / 9.3 | 48 | G538S (GGC→AGC) | ycgV | predicted adhesin |
| Reads supporting (aligned to +/- strand): ref base C (15/27); new base T (4/2); total (19/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01 | |||||||||||
CGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTG > AP009048/1255849‑1255988 | cGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGcc > 1:3770595/1‑70 (MQ=255) gTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaa > 1:2841092/1‑71 (MQ=255) tGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaac < 1:3450291/71‑1 (MQ=255) aTTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaacaa > 1:3742515/1‑71 (MQ=255) ttACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCaacaac < 1:331413/71‑1 (MQ=255) actactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCAc < 1:404644/71‑1 (MQ=255) ctactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACg < 1:2816867/71‑1 (MQ=255) ctactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACg > 1:341181/1‑71 (MQ=255) tCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaac < 1:319710/55‑1 (MQ=255) tCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCa < 1:4428800/62‑1 (MQ=255) cccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCataa > 1:1407069/1‑71 (MQ=255) ccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACAt > 1:3141649/1‑58 (MQ=255) gCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCataata > 1:4396520/1‑70 (MQ=255) cTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCa > 1:2883094/1‑60 (MQ=255) tGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGcc < 1:3954119/46‑1 (MQ=255) atTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTAc > 1:3356977/1‑71 (MQ=255) ttCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCat < 1:2632900/59‑1 (MQ=255) ttCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACt < 1:4586630/71‑1 (MQ=255) cAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAAAAACATCAGCACGCATAATAAAGGTACTGt > 1:3279517/1‑71 (MQ=255) aaTAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGt < 1:2884720/70‑1 (MQ=255) aaTAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGtt < 1:1947685/71‑1 (MQ=255) aaTAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGt < 1:846658/70‑1 (MQ=255) aTAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCa < 1:1987730/47‑1 (MQ=255) aTAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCACAATAAAGGTACTGTTa > 1:3362156/1‑71 (MQ=255) tAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGCTAc > 1:3723020/1‑71 (MQ=255) aaTCCCCTTTATTATTAACGCCATTACCCTCGCcaacaa > 1:1167761/1‑39 (MQ=255) aaTCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATc > 1:662518/1‑43 (MQ=255) aTCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGc > 1:3096975/1‑71 (MQ=255) tCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCt < 1:2101683/71‑1 (MQ=255) cccTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGtt < 1:412276/63‑1 (MQ=255) cccTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTc < 1:687652/70‑1 (MQ=255) cTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCat < 1:2537053/46‑1 (MQ=255) tttATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGt > 1:2998973/1‑71 (MQ=255) tattattaACGCCATTACCCTCGCCAACAACATCAGCACGCa > 1:1540714/1‑42 (MQ=255) attattaACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCt < 1:1962807/63‑1 (MQ=255) ttattaACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACt < 1:1967827/53‑1 (MQ=255) tattaACGCCATTACCCTCGCCAACAACATCAGCACGCAtaataa < 1:3315045/45‑1 (MQ=255) ttaACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTAc < 1:3539650/71‑1 (MQ=255) cGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGttt < 1:3228354/71‑1 (MQ=255) gCCATTACCCTCTCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTa < 1:2592614/71‑1 (MQ=255) ccATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTaa > 1:3032478/1‑71 (MQ=255) cATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAt < 1:4135751/71‑1 (MQ=255) cATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAt > 1:3238746/1‑71 (MQ=255) aTTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtg > 1:849037/1‑71 (MQ=255) aTTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtg < 1:3107298/71‑1 (MQ=255) ttACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtgt < 1:2243096/71‑1 (MQ=255) tACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTa > 1:1826412/1‑66 (MQ=255) aCCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTgg > 1:131246/1‑71 (MQ=255) ccTCGCCAACAACATCAGCACGCATAATAAAGGTACTGtt < 1:698528/40‑1 (MQ=255) ccTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCa < 1:1660150/71‑1 (MQ=255) tCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTAc < 1:1187884/56‑1 (MQ=255) cGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtg < 1:648229/63‑1 (MQ=255) cGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAAt > 1:2992511/1‑71 (MQ=255) gCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCaaa < 1:1580167/69‑1 (MQ=255) gCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAAt < 1:2516255/70‑1 (MQ=255) caacaaCATCAGCACGCATAATAAAGGTACTGTTACCGCTCa < 1:4565572/42‑1 (MQ=255) caacaaCATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAAtgtg < 1:3898544/71‑1 (MQ=255) | CGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTG > AP009048/1255849‑1255988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |