| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 1,028,434 | G→A | 14.8% | D23N (GAC→AAC) | yccU → | predicted CoA‑binding protein with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 1,028,434 | 0 | G | A | 14.8% | 68.8 / 4.7 | 27 | D23N (GAC→AAC) | yccU | predicted CoA‑binding protein with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (9/14); new base A (2/2); total (11/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
AATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCG > AP009048/1028364‑1028502 | aataatGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCg < 1:2480497/71‑1 (MQ=255) taatGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGAc < 1:4603151/71‑1 (MQ=255) taatGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGAc < 1:2233743/71‑1 (MQ=255) atGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACa > 1:3243702/1‑70 (MQ=255) tatTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTcc > 1:1061041/1‑69 (MQ=255) tatTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTcc > 1:55711/1‑69 (MQ=255) gCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATcg < 1:149767/70‑1 (MQ=255) cATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATcgcg < 1:633768/71‑1 (MQ=255) cATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATcgcg > 1:1913111/1‑71 (MQ=255) aTTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGt < 1:768623/71‑1 (MQ=255) gTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAat < 1:3849536/71‑1 (MQ=255) tCCACTCAAACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGATATCGCGTGATGaaa > 1:2273415/1‑69 (MQ=255) cacaCCATTGCGCTGGTGGGGGCAAGCAACAAACCCGAt > 1:54487/1‑39 (MQ=255) acacCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTAt > 1:384943/1‑50 (MQ=255) acacCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTc > 1:951001/1‑71 (MQ=255) acacCATTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTc > 1:4099232/1‑71 (MQ=255) cacCATTGCGCTGGTGGGGGCAAGCGACAAACCCg < 1:1815953/35‑1 (MQ=255) cATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGAc < 1:1702420/70‑1 (MQ=255) aTTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCa < 1:2707330/71‑1 (MQ=255) aTTGCGCTGGTGGGGGCAAGCAACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGAc < 1:4031094/69‑1 (MQ=255) gtggGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATc < 1:4477549/70‑1 (MQ=255) gtggGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATc < 1:915838/70‑1 (MQ=255) gtggGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTAATGAAATATCTACTCGACCAGGGGTATCa > 1:1173333/1‑71 (MQ=255) tggGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCa < 1:166402/70‑1 (MQ=255) tggGGGCAAGCAACAAACCCGATCGTCCCAGCTATCGCGtgatg < 1:3738402/44‑1 (MQ=255) gCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGAcc < 1:1469616/55‑1 (MQ=255) cAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCAt < 1:3324385/70‑1 (MQ=255) gCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCg > 1:508739/1‑71 (MQ=255) | AATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCG > AP009048/1028364‑1028502 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |