| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 711,161 | C→T | 13.7% | intergenic (‑93/+196) | fur ← / ← fldA | DNA‑binding transcriptional dual regulator/flavodoxin 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 711,161 | 0 | C | T | 13.7% | 138.7 / 11.6 | 51 | intergenic (‑93/+196) | fur/fldA | DNA‑binding transcriptional dual regulator/flavodoxin 1 |
| Reads supporting (aligned to +/- strand): ref base C (18/26); new base T (2/5); total (20/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAAC > AP009048/711097‑711231 | cACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTa < 1:351494/70‑1 (MQ=255) cTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAAt > 1:824989/1‑71 (MQ=255) cATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATg < 1:4208824/69‑1 (MQ=255) cATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGa > 1:22351/1‑70 (MQ=255) aTTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGaaa < 1:4465391/71‑1 (MQ=255) ttAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGaaaa > 1:3656884/1‑71 (MQ=255) agaagaAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAAc > 1:4514626/1‑70 (MQ=255) gACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGa < 1:4322025/57‑1 (MQ=255) aCAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTccc < 1:429856/70‑1 (MQ=255) aCAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTaaa > 1:2855099/1‑53 (MQ=255) cAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTccc < 1:1280913/69‑1 (MQ=255) gAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTgg > 1:4266469/1‑70 (MQ=255) aTTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCAc > 1:3439759/1‑41 (MQ=255) tGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTc > 1:904217/1‑60 (MQ=255) ctctTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAatat < 1:3779806/70‑1 (MQ=255) tctTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTccc < 1:3029215/59‑1 (MQ=255) ctTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCt < 1:3918144/59‑1 (MQ=255) ttGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGaaa < 1:435707/45‑1 (MQ=255) ttGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGaaa < 1:2554206/45‑1 (MQ=255) ttGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACcac < 1:957054/50‑1 (MQ=255) ttGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTg < 1:1876226/69‑1 (MQ=255) ttGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGt > 1:644259/1‑70 (MQ=255) ttGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGt > 1:880753/1‑70 (MQ=255) gagaTAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACcac > 1:1382428/1‑48 (MQ=255) gagaTAATGCGTATCATTATAGAATTGCCACGCCTAAAt > 1:217369/1‑39 (MQ=255) agaTAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTtaa > 1:3430533/1‑70 (MQ=255) agaTAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTtaa > 1:2647644/1‑70 (MQ=255) aTAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTtaataa < 1:602390/71‑1 (MQ=255) aTAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTtaataa < 1:3081043/71‑1 (MQ=255) tAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAatat < 1:4205212/61‑1 (MQ=255) tAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaa < 1:649565/71‑1 (MQ=255) tAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaa < 1:2255389/71‑1 (MQ=255) aaTGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACaa > 1:3078717/1‑45 (MQ=255) aaTGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAata < 1:553777/59‑1 (MQ=255) tGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAAc < 1:3057775/71‑1 (MQ=255) aTCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTgg > 1:3026248/1‑70 (MQ=255) tCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGca < 1:2072511/71‑1 (MQ=255) tCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGca < 1:2243379/71‑1 (MQ=255) cATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATAtt < 1:1938202/52‑1 (MQ=255) tataGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTg > 1:549432/1‑64 (MQ=255) ataGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCt > 1:1049716/1‑71 (MQ=255) gAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTa < 1:105370/70‑1 (MQ=255) gAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTAc < 1:3855468/71‑1 (MQ=255) aaTTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATAtt > 1:2433384/1‑44 (MQ=255) aTTGCCACGCCTAAATGAAAACCACAAGCCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACa < 1:1979981/70‑1 (MQ=255) ttGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAata > 1:1108196/1‑40 (MQ=255) gCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACAttt < 1:1660609/70‑1 (MQ=255) ccATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTAc < 1:3507686/71‑1 (MQ=255) ccACGCCTAAATGAAAACCACTAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTAc < 1:3110184/71‑1 (MQ=255) ccACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGc < 1:4254604/56‑1 (MQ=255) aCGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTta > 1:1680846/1‑41 (MQ=255) aCGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACaa < 1:2071607/71‑1 (MQ=255) cGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTtaataa > 1:3144645/1‑44 (MQ=255) cGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAAc > 1:4055013/1‑71 (MQ=255) | CACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAAC > AP009048/711097‑711231 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |