| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 70,581 | C→T | 44.6% | V65V (GTC→GTT) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 70,581 | 0 | C | T | 44.6% | 28.5 / 96.4 | 74 | V65V (GTC→GTT) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (22/19); new base T (17/16); total (39/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01 | |||||||||||
TGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCT > AP009048/70511‑70650 | tGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTc > 1:3469461/1‑71 (MQ=255) gAAAGGTTATATTCTCAATCTCACCATTCGCGGTCA‑GGGGTGGTGAAAAATCAGGGACGAGAAtttgtttg > 1:3084593/1‑71 (MQ=255) aGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGcc > 1:2799646/1‑71 (MQ=255) aGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGcc > 1:4337396/1‑71 (MQ=255) atatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGAcc < 1:2937488/70‑1 (MQ=255) ttCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAcc < 1:242369/67‑1 (MQ=255) tctcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGt > 1:2593217/1‑70 (MQ=255) tcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTc > 1:3156742/1‑56 (MQ=255) tcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGc > 1:4112899/1‑59 (MQ=255) tcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGc > 1:1184915/1‑59 (MQ=255) tcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGa > 1:397998/1‑70 (MQ=255) cAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGata > 1:868576/1‑71 (MQ=255) aaTCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGata < 1:3834933/70‑1 (MQ=255) aaTCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGata < 1:4471382/70‑1 (MQ=255) aTCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAtt < 1:4512820/71‑1 (MQ=255) aTCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAtt < 1:1991548/71‑1 (MQ=255) aTCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAtt < 1:755675/71‑1 (MQ=255) cACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc > 1:1550968/1‑71 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAttt < 1:117282/67‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAtttt < 1:977001/68‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAttt < 1:3573905/67‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg < 1:1137138/69‑1 (MQ=255) ccATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTg > 1:770170/1‑71 (MQ=255) ttCGCGTTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTAGCCGACCGGGTGATAttt < 1:3186638/63‑1 (MQ=255) ttCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGtt < 1:2001120/70‑1 (MQ=255) gcgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCg > 1:926372/1‑71 (MQ=255) tCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:4519573/69‑1 (MQ=255) tCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg < 1:4386767/71‑1 (MQ=255) cAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:1597527/1‑70 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTg < 1:2504753/48‑1 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGga < 1:516455/71‑1 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGAcc > 1:3988227/1‑43 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGa > 1:3109409/1‑49 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAttt < 1:3344158/54‑1 (MQ=255) gggggTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGtt < 1:2978233/60‑1 (MQ=255) gggggTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:4384859/1‑68 (MQ=255) ggggTGGTGAAAAATCAGGGACGAGATTTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAgg > 1:3679070/1‑68 (MQ=255) ggggTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCa < 1:1611938/66‑1 (MQ=255) ggtggtGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGata < 1:2759234/47‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGc < 1:57839/60‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:2905905/67‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGtt < 1:2877828/55‑1 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAtt > 1:260340/1‑45 (MQ=255) ttaaaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagag < 1:3867463/63‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:3478492/71‑1 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGt > 1:2399970/1‑37 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGga > 1:2505309/1‑61 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:1375464/1‑71 (MQ=255) aaaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAtttt > 1:770999/1‑44 (MQ=255) aaaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAtt > 1:3616409/1‑42 (MQ=255) aaaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTTCCGCCAGGAGAGATtcatca > 1:491962/1‑71 (MQ=255) aaaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:3571898/1‑71 (MQ=255) aaaaaTCAGGGACGAGAATTTCTCTGCCGACCGGGTGATATTTTg < 1:4293974/45‑1 (MQ=255) aaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat > 1:4182739/1‑67 (MQ=255) aTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAtt > 1:911031/1‑38 (MQ=255) tCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTa > 1:1180546/1‑69 (MQ=255) tCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACg > 1:961243/1‑71 (MQ=255) tCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGACATTCATCACTACg < 1:1784185/71‑1 (MQ=255) tCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACg < 1:98175/71‑1 (MQ=255) aGGTACGAGAATTTGTTTGCCGACCGGGTGATAttt > 1:4008769/1‑36 (MQ=37) aGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgt > 1:1163611/1‑71 (MQ=255) aGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt > 1:1060234/1‑59 (MQ=255) aGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt > 1:1810075/1‑59 (MQ=255) ggACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtcg > 1:4101785/1‑71 (MQ=255) gACGAGAATTTGTTTGCCGACCGGGTGATATTTTg < 1:3948593/35‑1 (MQ=255) gACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTccc > 1:2385223/1‑43 (MQ=255) gACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtcgt < 1:1819016/71‑1 (MQ=255) cGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtc < 1:3148290/55‑1 (MQ=255) cGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCa > 1:3750529/1‑71 (MQ=255) gagaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCAt > 1:3930979/1‑71 (MQ=255) gagaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCAt < 1:1409514/71‑1 (MQ=255) aaTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtc < 1:4410197/51‑1 (MQ=255) aaTTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACgg > 1:366747/1‑61 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg > 1:1959867/1‑70 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgag > 1:4196500/1‑43 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg > 1:2366369/1‑69 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTa > 1:741201/1‑54 (MQ=255) gTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGga > 1:2013148/1‑39 (MQ=255) tCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCt < 1:4098528/71‑1 (MQ=255) | TGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCT > AP009048/70511‑70650 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |