| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 647,863 | A→G | 8.4% | M311T (ATG→ACG) | citF ← | citrate lyase, citrate‑ACP transferase (alpha) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 647,863 | 0 | A | G | 8.4% | 194.0 / 8.5 | 71 | M311T (ATG→ACG) | citF | citrate lyase, citrate‑ACP transferase (alpha) subunit |
| Reads supporting (aligned to +/- strand): ref base A (33/32); new base G (2/4); total (35/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAG > AP009048/647793‑647930 | cgcAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCa < 1:3946051/71‑1 (MQ=255)cgcAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCa < 1:1652496/71‑1 (MQ=255) caTGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtcgtc < 1:3649155/71‑1 (MQ=255) tGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTcgc < 1:891202/71‑1 (MQ=255) tGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTcgc > 1:2347317/1‑71 (MQ=255) gCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtc < 1:744589/64‑1 (MQ=255) tCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTaa < 1:3403736/70‑1 (MQ=255) aaaGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTaa < 1:1985416/68‑1 (MQ=255) aaaGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATa < 1:3954781/70‑1 (MQ=255) aaaGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATAc > 1:1636460/1‑71 (MQ=255) aaaGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATAc < 1:533607/71‑1 (MQ=255) aGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTcgc < 1:4298971/61‑1 (MQ=255) ctctGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGcc < 1:2025050/71‑1 (MQ=255) cacaTCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTAATACCGCCaa < 1:2238542/68‑1 (MQ=255) acaTCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCg > 1:400496/1‑63 (MQ=255) acaTCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGc < 1:633915/71‑1 (MQ=255) tCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTAATACCGCCAAGGGCg > 1:717512/1‑69 (MQ=255) tCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAAt < 1:135720/56‑1 (MQ=255) ccAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAg > 1:3401269/1‑71 (MQ=255) ccAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAg > 1:797009/1‑71 (MQ=255) cagcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGaa > 1:73125/1‑69 (MQ=255) cagcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGt < 1:1128217/71‑1 (MQ=255) agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGcc < 1:1426101/59‑1 (MQ=255) cagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAAt < 1:2843634/51‑1 (MQ=255) agTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAg < 1:3897581/59‑1 (MQ=255) tttGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtcgtc < 1:112205/40‑1 (MQ=255) tttGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCgg < 1:3926353/44‑1 (MQ=255) tttGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGgcgc < 1:4370977/71‑1 (MQ=255) tttGAGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGgcgc > 1:1879312/1‑71 (MQ=255) tGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtc > 1:104599/1‑35 (MQ=255) tGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCgg > 1:776938/1‑42 (MQ=255) tGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCaa > 1:1051816/1‑54 (MQ=255) ggATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAat > 1:3627927/1‑70 (MQ=255) gATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTaa < 1:174543/41‑1 (MQ=255) aTCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCg > 1:2214548/1‑60 (MQ=255) tCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCaa > 1:4394330/1‑48 (MQ=255) tCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGaa > 1:91139/1‑55 (MQ=255) tCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATcg < 1:40304/71‑1 (MQ=255) cAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCg < 1:2551821/52‑1 (MQ=255) cAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGgcgc > 1:3708974/1‑62 (MQ=255) cAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATcgc > 1:3287860/1‑71 (MQ=255) aGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATcgc < 1:2923624/70‑1 (MQ=255) aGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGt < 1:3621924/35‑1 (MQ=255) aGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATcgcg > 1:2218218/1‑71 (MQ=255) aGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATcgcg < 1:2888333/71‑1 (MQ=255) aGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATcgc < 1:276234/70‑1 (MQ=255) aGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATcgc < 1:4493736/70‑1 (MQ=255) aCCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCa < 1:1431666/43‑1 (MQ=255) aCCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCa < 1:1281382/43‑1 (MQ=255) aCCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGc > 1:4160137/1‑71 (MQ=255) aCCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGc > 1:4165694/1‑71 (MQ=255) ccTTTTTCGTGCAGGTCAACCGTCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCt < 1:3146755/71‑1 (MQ=255) ccTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGCGCGAAGTCGGCGCGAATATcgccgc > 1:553210/1‑70 (MQ=255) cTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTa > 1:1172715/1‑71 (MQ=255) cTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTa > 1:4197639/1‑71 (MQ=255) cTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTa > 1:4558070/1‑71 (MQ=255) ttttCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATcg > 1:240188/1‑63 (MQ=255) ttttCGTGCAGGTCAACAATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGgcgc > 1:425744/1‑55 (MQ=255) tttCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAgg < 1:3897486/41‑1 (MQ=255) tttCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACg > 1:2506116/1‑70 (MQ=255) tttCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACg > 1:634324/1‑70 (MQ=255) tttCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACg > 1:1163493/1‑70 (MQ=255) ttCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGc < 1:3719397/70‑1 (MQ=255) cGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGc > 1:682620/1‑63 (MQ=255) cGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCAt < 1:1142035/70‑1 (MQ=255) aGGTCAACCGTCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTc > 1:4591857/1‑71 (MQ=255) gTCAACCGTCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCt < 1:1873568/70‑1 (MQ=255) gTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCa > 1:4187581/1‑62 (MQ=255) tCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTAc < 1:1181524/58‑1 (MQ=255) aaCCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCgg < 1:2904085/52‑1 (MQ=255) aaCCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTc > 1:81215/1‑69 (MQ=255) ccATCGTCGCTGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAg > 1:1768004/1‑70 (MQ=255) ccATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAg > 1:1469857/1‑70 (MQ=255) | CGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAG > AP009048/647793‑647930 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |