| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 464,946 | A→G | 10.0% | F531L (TTC→CTC) | ybaE ← | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 464,946 | 0 | A | G | 10.0% | 130.0 / 7.2 | 50 | F531L (TTC→CTC) | ybaE | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (24/21); new base G (2/3); total (26/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
GCGGTGTCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGTTTTC > AP009048/464886‑465015 | gcgGTGTCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGaa > 1:3948246/1‑61 (MQ=255) gtgtCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGt < 1:1448725/67‑1 (MQ=255) gtCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGaa > 1:4250924/1‑56 (MQ=255) aGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCg > 1:2191488/1‑70 (MQ=255) tCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCAt > 1:3427904/1‑71 (MQ=255) tCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCAt > 1:2215012/1‑71 (MQ=255) cGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc > 1:1553797/1‑71 (MQ=255) gCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGGGGCGTCAGCGTCGCATc > 1:170120/1‑70 (MQ=255) acacCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACa > 1:3018934/1‑49 (MQ=255) acacCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACa > 1:1840639/1‑49 (MQ=255) acacCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGt < 1:161303/71‑1 (MQ=255) acCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCa > 1:4100418/1‑71 (MQ=255) acCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCa > 1:4062482/1‑71 (MQ=255) ccGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGaa > 1:127906/1‑44 (MQ=255) aCGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACt < 1:1392153/70‑1 (MQ=255) aCGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACt < 1:4147932/70‑1 (MQ=255) cGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGt > 1:4132945/1‑61 (MQ=255) cGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGt < 1:1042891/61‑1 (MQ=255) cGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTgg > 1:3778025/1‑71 (MQ=255) cGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTgg > 1:1567176/1‑71 (MQ=255) cGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTgg > 1:3529347/1‑71 (MQ=255) ccGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCt < 1:2771105/71‑1 (MQ=255) ccGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGc > 1:2440603/1‑70 (MQ=255) ccGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGc > 1:3543150/1‑70 (MQ=255) ccGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGGCATTAACTGGc > 1:3642827/1‑70 (MQ=255) cGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATc > 1:865471/1‑55 (MQ=255) cGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCt > 1:2928240/1‑70 (MQ=255) gggAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaa < 1:1191473/71‑1 (MQ=255) gggAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaa < 1:903520/71‑1 (MQ=255) ggAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaa < 1:2168975/71‑1 (MQ=255) ggAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaa < 1:1391227/71‑1 (MQ=255) ggAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaa > 1:2279010/1‑70 (MQ=255) cACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaa < 1:982544/64‑1 (MQ=255) aCTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaaaa > 1:1368337/1‑65 (MQ=255) tAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCa < 1:1538343/41‑1 (MQ=255) aaTGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCAtt < 1:4174503/49‑1 (MQ=255) aaTGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCAtt < 1:3116040/49‑1 (MQ=255) aTGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAg > 1:4556739/1‑71 (MQ=255) gCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACt < 1:461070/50‑1 (MQ=255) gCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCtt < 1:1097777/65‑1 (MQ=255) cGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCa > 1:3597203/1‑67 (MQ=255) atagtgatagtTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCAt < 1:2615810/71‑1 (MQ=255) atagtgatagtTGAACAGCGGCGGCAGCGTCGCATCTg < 1:4047125/38‑1 (MQ=255) agtgatagtTGAACAGCGGCGTCAGCGTCGCATCTGTCAtt > 1:2339126/1‑41 (MQ=255) gtgatagtTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTa > 1:3739687/1‑70 (MQ=255) tgatagtTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTc < 1:2142985/60‑1 (MQ=255) gatagtTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTaa > 1:3831331/1‑69 (MQ=255) atagtTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTaaa < 1:1969776/69‑1 (MQ=255) atagtTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATc < 1:4346635/71‑1 (MQ=255) tagtTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCg > 1:1822023/1‑71 (MQ=255) ttGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACt < 1:1054382/37‑1 (MQ=255) tGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGtt < 1:100247/70‑1 (MQ=255) gAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGttt < 1:1167103/70‑1 (MQ=255) aaCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGTTttc > 1:169419/1‑71 (MQ=255) | GCGGTGTCAGTCGCACACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGTTTTC > AP009048/464886‑465015 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |