| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 345,099 | C→T | 17.5% | A155T (GCC→ACC) | yahN ← | neutral amino‑acid efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 345,099 | 0 | C | T | 17.5% | 94.7 / 12.9 | 40 | A155T (GCC→ACC) | yahN | neutral amino‑acid efflux system |
| Reads supporting (aligned to +/- strand): ref base C (17/16); new base T (5/2); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01 | |||||||||||
CGCCAGATAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAAT > AP009048/345032‑345165 | cGCCAGATAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTAtt > 1:869408/1‑71 (MQ=255) gCCAGATAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCAttt < 1:1388831/71‑1 (MQ=255) gCCAGATAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCAtt < 1:3124530/70‑1 (MQ=255) cAGATAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTaa < 1:3258829/71‑1 (MQ=255) aTAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGt < 1:4581737/71‑1 (MQ=255) aaTTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTa > 1:2288863/1‑71 (MQ=255) aTTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa > 1:1875538/1‑65 (MQ=255) aTTGATGCGAGCACAATCCCCGCCCAGCCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTAc > 1:243109/1‑71 (MQ=255) ttGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATg < 1:2172757/66‑1 (MQ=255) ttGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACt < 1:2764373/71‑1 (MQ=255) gATGCGAGCACAATCCCAGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTa > 1:102197/1‑61 (MQ=255) caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACt > 1:4268223/1‑70 (MQ=255) ccccGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATa > 1:918348/1‑70 (MQ=255) gCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACt < 1:3264549/62‑1 (MQ=255) gCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTa > 1:108536/1‑43 (MQ=255) gCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTa > 1:2190343/1‑49 (MQ=255) cccAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGaa < 1:1888220/55‑1 (MQ=255) ccAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATa < 1:3950657/71‑1 (MQ=255) cAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATaa < 1:2327164/71‑1 (MQ=255) aGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATaa > 1:3366467/1‑70 (MQ=255) ggCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGAt > 1:3723162/1‑60 (MQ=255) gCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAAc > 1:347622/1‑71 (MQ=255) ccATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACg < 1:328869/71‑1 (MQ=255) aTTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACt > 1:3581760/1‑53 (MQ=255) ttAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTgag < 1:710651/44‑1 (MQ=255) ttAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGtt < 1:3596709/71‑1 (MQ=255) aaaCGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTgaga > 1:3573867/1‑43 (MQ=255) aaaCGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATa > 1:4551807/1‑48 (MQ=255) aaCGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATa > 1:1668086/1‑47 (MQ=255) aCGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTga > 1:3798761/1‑39 (MQ=255) cccATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGtt < 1:863949/70‑1 (MQ=255) cATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTaga < 1:3221137/71‑1 (MQ=255) cATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTaga < 1:1939953/71‑1 (MQ=255) ttGGTGTTTCGGCATTTAATGTTACTGAGAAAATa > 1:3733553/1‑35 (MQ=255) ttGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTaga > 1:3788813/1‑67 (MQ=255) ggTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGAt < 1:2359851/70‑1 (MQ=255) ggTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGAt < 1:2580889/70‑1 (MQ=255) ttCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATc > 1:2157992/1‑66 (MQ=255) tCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGtaat > 1:2737932/1‑71 (MQ=255) cGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTaga > 1:2974017/1‑58 (MQ=255) | CGCCAGATAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAAT > AP009048/345032‑345165 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |