| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 4,392,088 | G→T | 20.8% | L655M (CTG→ATG) | yjeP ← | predicted mechanosensitive channel |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 4,392,088 | 0 | G | T | 20.8% | 136.2 / 16.5 | 48 | L655M (CTG→ATG) | yjeP | predicted mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base G (14/24); new base T (6/4); total (20/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCAATCATCATGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCA > AP009048/4392019‑4392154 | cgcCAATCATCATGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGc < 1:730761/71‑1 (MQ=255) aaTCATCATGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGaa > 1:2007172/1‑71 (MQ=255) atcatGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATccc > 1:1964165/1‑71 (MQ=255) atcatGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATccc > 1:1821039/1‑71 (MQ=255) tcatGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCg > 1:2080798/1‑71 (MQ=255) atGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAg > 1:1003185/1‑59 (MQ=255) tGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGcc < 1:1203841/71‑1 (MQ=255) ttCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCtt > 1:1146950/1‑71 (MQ=255) ttCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCt < 1:1239297/70‑1 (MQ=255) ccACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCtttt > 1:546557/1‑71 (MQ=255) ccACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCttt < 1:1559972/70‑1 (MQ=255) ccACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCttt < 1:2528720/70‑1 (MQ=255) cacaGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCttttt < 1:811965/71‑1 (MQ=255) cacaGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCttttt < 1:2337439/71‑1 (MQ=255) cacaGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCttttt < 1:1296217/71‑1 (MQ=255) acaGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATc > 1:3822284/1‑59 (MQ=255) atatGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCt < 1:1325152/71‑1 (MQ=255) tggttggtAATGTTTTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGa < 1:494221/70‑1 (MQ=255) tggttggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCAGGCCTTTTTCAGGCTGa < 1:2838400/70‑1 (MQ=255) gttggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAggg > 1:1823007/1‑71 (MQ=255) ttggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGt < 1:575450/71‑1 (MQ=255) gtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGac < 1:617353/71‑1 (MQ=255) gtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGac > 1:3437179/1‑71 (MQ=255) gtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGa < 1:808887/70‑1 (MQ=255) tAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTg < 1:720221/62‑1 (MQ=255) tAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGac < 1:2129614/70‑1 (MQ=255) aTGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGc > 1:3661787/1‑58 (MQ=255) ttgtCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCttttt > 1:915819/1‑50 (MQ=255) gtCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCgg > 1:391308/1‑41 (MQ=255) gtCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGcc < 1:231652/70‑1 (MQ=255) gtCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGc > 1:4051022/1‑69 (MQ=255) gcTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCtttt < 1:2829127/41‑1 (MQ=255) gcTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCAc > 1:2893986/1‑71 (MQ=255) ccTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAggg < 1:545726/48‑1 (MQ=255) tctTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGa > 1:4361409/1‑43 (MQ=255) ctTTGTTGAGATACATCGGAATCCCGGCCTTTTTCa > 1:1214659/1‑36 (MQ=255) ctTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGc > 1:792910/1‑39 (MQ=255) tttGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAggg < 1:3898920/44‑1 (MQ=255) tttGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAAt > 1:3927784/1‑69 (MQ=255) tttGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAAt < 1:1488310/69‑1 (MQ=255) tgttgAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGa < 1:430109/69‑1 (MQ=255) tgAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGa > 1:1455479/1‑69 (MQ=255) gagaTACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCaa < 1:2485354/61‑1 (MQ=255) aTACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaag < 1:586780/70‑1 (MQ=255) tACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaagca < 1:1553976/71‑1 (MQ=255) tACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaagca < 1:3353625/71‑1 (MQ=255) tACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaagca < 1:265438/71‑1 (MQ=255) tACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaagca < 1:960873/71‑1 (MQ=255) aCATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaagca > 1:3826369/1‑70 (MQ=255) | CGCCAATCATCATGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCA > AP009048/4392019‑4392154 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |