| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,825,123 | C→G | 100% | R159R (CGC→CGG) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,825,123 | 0 | C | G | 100.0% | 197.7 / NA | 56 | R159R (CGC→CGG) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (19/37); total (19/37) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch. | |||||||||||
GGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGGAACGCGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATC > AP009048/3825057‑3825189 | ggAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgc > 1:3189050/1‑71 (MQ=255) gagaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTTATCGTCAGGAACGGGGgcg < 1:32197/71‑1 (MQ=255) gagaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgc < 1:927143/70‑1 (MQ=255) agaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgcgc > 1:1673456/1‑71 (MQ=255) agaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgcgc < 1:3545027/71‑1 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACgggg > 1:1208203/1‑66 (MQ=255) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc < 1:2763703/71‑1 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCgag < 1:1555226/71‑1 (MQ=255) aGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAtt < 1:1617157/71‑1 (MQ=255) ggCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAttt < 1:4522319/71‑1 (MQ=255) ggCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAttt < 1:3087504/71‑1 (MQ=255) ggCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAttt < 1:3627377/71‑1 (MQ=255) ggCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGGCAGGATCGGGGGCGCGGCGAGAt < 1:620104/69‑1 (MQ=255) gctaaTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTc < 1:1221193/68‑1 (MQ=255) gctaaTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTc < 1:2935298/68‑1 (MQ=255) gctaaTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTc < 1:3418387/68‑1 (MQ=255) aTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGc > 1:4119517/1‑70 (MQ=255) aTGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGc < 1:4265230/70‑1 (MQ=255) gcgcGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGc < 1:305839/67‑1 (MQ=255) gcgcGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGAtt < 1:72610/71‑1 (MQ=255) cgcgACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTc < 1:1016784/71‑1 (MQ=255) gcgACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGAtt > 1:1325275/1‑69 (MQ=255) gACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAgg < 1:1107995/71‑1 (MQ=255) aCGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGAtt < 1:207328/66‑1 (MQ=255) ttGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGa < 1:3587500/60‑1 (MQ=255) cTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCa < 1:894915/61‑1 (MQ=255) ggCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGc > 1:4297877/1‑70 (MQ=255) gCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCg < 1:2333377/70‑1 (MQ=255) tattGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCgaga < 1:2541920/40‑1 (MQ=37) tattGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTg < 1:2278894/61‑1 (MQ=255) attGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGAt > 1:3945307/2‑70 (MQ=255) ttGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCgaga > 1:3202154/1‑40 (MQ=38) ttGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCgg > 1:384887/1‑46 (MQ=255) ttGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTa > 1:4006316/1‑71 (MQ=255) tGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAg > 1:820145/1‑57 (MQ=255) gATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTAc > 1:2426494/1‑70 (MQ=255) aTTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCgg < 1:2537616/43‑1 (MQ=38) aTTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACaa < 1:748236/71‑1 (MQ=255) aTTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACaa < 1:3483607/71‑1 (MQ=255) tttCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGc > 1:2375551/1‑43 (MQ=38) ttCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGAtt < 1:1624203/46‑1 (MQ=255) gATCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCaaa < 1:1799145/69‑1 (MQ=255) aTCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTg < 1:3599589/71‑1 (MQ=255) tCGTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCa < 1:3544199/65‑1 (MQ=255) gTCAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACaa > 1:4069892/1‑58 (MQ=255) cAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCa < 1:784673/61‑1 (MQ=255) cAGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGAtctc > 1:1534330/1‑71 (MQ=255) aGGAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATctct < 1:3954948/71‑1 (MQ=255) ggAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTa < 1:4268683/71‑1 (MQ=255) gAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTa < 1:2984781/70‑1 (MQ=255) gAACGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTa > 1:4384461/1‑70 (MQ=255) aaCGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGAtctc > 1:2452095/1‑67 (MQ=255) aaCGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTatc < 1:1195985/71‑1 (MQ=255) aaCGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTatc < 1:223702/71‑1 (MQ=255) aCGGGGGCGCGGGGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTat > 1:2843365/1‑69 (MQ=255) aCGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCg > 1:70704/1‑42 (MQ=255) aCGGGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACaa > 1:2580791/1‑51 (MQ=255) | GGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGGAACGCGGGCGCGGCGAGATTTCGGCGATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATC > AP009048/3825057‑3825189 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |