| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,824,913 | C→T | 100% | H89H (CAC→CAT) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,824,913 | 0 | C | T | 100.0% | 193.2 / NA | 65 | H89H (CAC→CAT) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (30/35); total (30/35) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAAGAAGCAAAAGACCACGGTGAAGGCGGCAATCTGG > AP009048/3824845‑3824980 | ctgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATga > 1:2549689/1‑71 (MQ=255) tgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgac > 1:535883/1‑71 (MQ=255) tgTTTCGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgac > 1:4275619/1‑71 (MQ=255) ttGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgacctt > 1:914818/1‑70 (MQ=255) tGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgacctt < 1:723396/70‑2 (MQ=255) ggCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttg < 1:946871/70‑3 (MQ=255) cccTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATga < 1:1173242/63‑1 (MQ=255) cTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctg > 1:4154892/1‑71 (MQ=255) cTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg < 1:2477700/71‑1 (MQ=255) cTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg < 1:3952206/71‑1 (MQ=255) cTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg > 1:4034216/1‑71 (MQ=255) aCAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttga > 1:4554861/1‑61 (MQ=255) aCAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttg < 1:1502046/60‑3 (MQ=255) aGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt > 1:2749567/1‑71 (MQ=255) aGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt > 1:1022278/1‑71 (MQ=255) ggATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgacctt > 1:3127028/1‑52 (MQ=25) gATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTa < 1:1466832/71‑1 (MQ=255) gATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTa < 1:4614601/71‑1 (MQ=255) cccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAAcc > 1:440643/1‑71 (MQ=255) cccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAAcc > 1:4509904/1‑71 (MQ=255) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCg > 1:962894/1‑71 (MQ=255) gATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTAc > 1:3427009/1‑58 (MQ=255) gATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGc > 1:1161898/1‑70 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaa < 1:4526239/71‑1 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCa > 1:4159049/1‑70 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa < 1:1225498/71‑1 (MQ=255) gcgaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaag < 1:3971688/67‑1 (MQ=255) cgaccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaaga < 1:2718398/68‑1 (MQ=255) accacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaagagg > 1:2062596/1‑68 (MQ=255) ccacTACCGCCGTGGCGCTGGCGGAGCATCATgacctt < 1:3581350/38‑2 (MQ=16) ccacTACCGCCGTGGCGCTGGCGGAGCATCATgacctt < 1:595433/38‑2 (MQ=16) ccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc > 1:1877022/1‑70 (MQ=255) ccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCa < 1:45416/71‑1 (MQ=255) cactACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaagag < 1:1000237/67‑2 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGc < 1:2567848/48‑1 (MQ=25) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:861457/69‑1 (MQ=255) taCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa > 1:699391/2‑68 (MQ=255) aCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAAcc > 1:2805475/1‑55 (MQ=255) aCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGa > 1:3531944/1‑71 (MQ=255) ccgccgTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGAc > 1:301170/1‑71 (MQ=255) cGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGAc > 1:2433211/1‑70 (MQ=255) ccgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:2300501/63‑1 (MQ=255) cgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGa > 1:787647/2‑66 (MQ=255) cgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACg > 1:3542628/2‑71 (MQ=255) gtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAAcc > 1:1717083/1‑49 (MQ=38) tgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGt < 1:1153678/71‑1 (MQ=255) tgGCGCTGGCAGAGCATCATGACCTTGACCTGCCGTACTGCtt < 1:4030354/43‑1 (MQ=25) gcgcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTg < 1:4145690/67‑1 (MQ=255) gcgcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTg > 1:1464760/4‑70 (MQ=255) gcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGaa < 1:1666346/69‑1 (MQ=255) cTGGCGGAGCATCATGTCCTTGACCTGCCGTACTGCTTTAACCGCaaa < 1:3357981/48‑1 (MQ=255) cTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAgg < 1:2756953/71‑1 (MQ=255) cTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGA‑CACGGTg < 1:4227687/66‑1 (MQ=255) ggCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTg < 1:3488427/65‑1 (MQ=255) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcg < 1:4102944/70‑1 (MQ=255) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcg < 1:6348/70‑1 (MQ=255) cGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcggc < 1:4521222/71‑1 (MQ=255) gAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAaagagg > 1:2255971/1‑44 (MQ=255) gAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCaa > 1:2592744/1‑71 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa < 1:1047800/41‑1 (MQ=38) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAAt < 1:354690/71‑1 (MQ=255) catcatGACCTTGACCTGCCGTACTGCTTTAACCGCAaaga < 1:3790835/41‑1 (MQ=38) aTCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtg < 1:580753/71‑1 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCa < 1:842187/35‑1 (MQ=37) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtgg > 1:1549055/1‑71 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtgg < 1:3171544/71‑1 (MQ=255) | CTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAAGAAGCAAAAGACCACGGTGAAGGCGGCAATCTGG > AP009048/3824845‑3824980 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |