| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,824,880 | C→G | 100% | A78A (GCC→GCG) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,824,880 | 0 | C | G | 100.0% | 197.0 / NA | 58 | A78A (GCC→GCG) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (30/28); total (30/28) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAA > AP009048/3824812‑3824946 | gcattGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcgac < 1:1600689/68‑1 (MQ=255) cattGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcgacc < 1:732104/69‑1 (MQ=255) attGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcgacca > 1:4037447/2‑71 (MQ=255) ttGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCgaccac < 1:3313188/71‑1 (MQ=255) ttGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCgaccac < 1:2469207/71‑1 (MQ=255) ggtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcgacca > 1:4353015/1‑68 (MQ=255) ggtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCgaccact < 1:1173327/70‑2 (MQ=255) gtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCgaccacta < 1:2385354/70‑3 (MQ=255) ggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcgacca > 1:4082331/1‑65 (MQ=255) aTTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGcc > 1:1773308/1‑70 (MQ=255) gCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCcgtg < 1:4048882/68‑1 (MQ=255) gCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGcg > 1:2605870/1‑70 (MQ=255) aTTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCt < 1:3406179/71‑1 (MQ=255) gAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTgg < 1:4170334/70‑1 (MQ=255) gAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTgg < 1:884798/70‑1 (MQ=255) gAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGc > 1:3684528/1‑71 (MQ=255) aGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGct < 1:1566706/71‑2 (MQ=255) gTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCgg < 1:1119110/71‑1 (MQ=255) gTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCgg < 1:369087/71‑1 (MQ=255) cGATCTGCTGTTTGGCCCTGATTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAg > 1:4340154/1‑70 (MQ=255) cGATCTGCTGTTTGGCCCTGATTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAg > 1:4345456/1‑70 (MQ=255) ctgctgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGcg > 1:1508981/1‑56 (MQ=255) ctgctgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCa > 1:2036439/1‑71 (MQ=255) ctgctgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCa > 1:2908663/1‑71 (MQ=255) ctgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCa < 1:1666986/65‑1 (MQ=255) ctgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCatg < 1:4338451/70‑3 (MQ=255) ctgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCa < 1:4464989/68‑1 (MQ=255) ctgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCa < 1:4097109/68‑1 (MQ=255) ctgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATga > 1:2549689/1‑71 (MQ=255) tgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAg > 1:4157981/1‑62 (MQ=255) tgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCa < 1:3294156/64‑1 (MQ=255) tgTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgac > 1:535883/1‑71 (MQ=255) tgTTTCGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgac > 1:4275619/1‑71 (MQ=255) ttGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgacctt > 1:914818/1‑70 (MQ=255) tGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATc > 1:402277/1‑62 (MQ=255) tGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATc > 1:1155934/1‑62 (MQ=255) tGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgacctt < 1:723396/70‑2 (MQ=255) ggCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttg < 1:946871/70‑3 (MQ=255) gCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGa < 1:1834914/55‑1 (MQ=255) cccTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATga < 1:1173242/63‑1 (MQ=255) cTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGc < 1:980639/43‑1 (MQ=37) cTGCTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttgacctg > 1:4154892/1‑71 (MQ=255) cTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGa < 1:3218598/49‑1 (MQ=37) cTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg < 1:3952206/71‑1 (MQ=255) cTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg > 1:4034216/1‑71 (MQ=255) cTTACAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCg < 1:2477700/71‑1 (MQ=255) aCAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttga > 1:4554861/1‑61 (MQ=255) aCAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgaccttg < 1:1502046/60‑3 (MQ=255) cAAAGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCa < 1:3650222/48‑1 (MQ=37) aaaGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCatg > 1:3571818/1‑50 (MQ=37) aaGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCAt > 1:3408831/1‑47 (MQ=25) aGGGATCCCGATTGCGACCACTACCGCCGTGGCGCt > 1:2958867/1‑36 (MQ=16) aGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt > 1:1022278/1‑71 (MQ=255) aGGGATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCt > 1:2749567/1‑71 (MQ=255) ggATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATgacctt > 1:3127028/1‑52 (MQ=25) gATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTa < 1:4614601/71‑1 (MQ=255) gATCCCGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTa < 1:1466832/71‑1 (MQ=255) cccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAAcc > 1:4509904/1‑71 (MQ=255) cccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAAcc > 1:440643/1‑71 (MQ=255) ccGATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCg > 1:962894/1‑71 (MQ=255) gATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCat < 1:65220/40‑2 (MQ=21) gATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTAc > 1:3427009/1‑58 (MQ=255) gATTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGc > 1:1161898/1‑70 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCa < 1:2744902/35‑1 (MQ=16) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaa < 1:4526239/71‑1 (MQ=255) aTTGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCa > 1:4159049/1‑70 (MQ=255) ttGCGACCACTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCaaa < 1:1225498/71‑1 (MQ=255) | GCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAA > AP009048/3824812‑3824946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |